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Preparation method of tea polyphenols in Southwest Camellia

A camellia and tea polyphenol technology is applied in the field of preparation of active components in natural medicine Southwest camellia, which can solve the problems of unfavorable development and utilization of Southwest camellia, affecting the composition and structure of total polyphenols, and low yield of extracts, etc. The effect of improving efficiency and yield, low cost and high purity

Active Publication Date: 2020-09-22
DALIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Therefore, it is necessary to conduct in-depth research on the extraction, purification and enrichment process of tea polyphenols from Southwest Camellia. Enzyme, ultrasound and other treatments will affect the composition and structure of total polyphenols, the yield of the extract is low, and there are more impurities, which brings great difficulty to the follow-up treatment, is time-consuming and laborious, and is not conducive to the development of the medicinal value of Southwest Camellia. and use

Method used

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  • Preparation method of tea polyphenols in Southwest Camellia
  • Preparation method of tea polyphenols in Southwest Camellia
  • Preparation method of tea polyphenols in Southwest Camellia

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preparation example Construction

[0026] The specific embodiment of the preparation method of tea polyphenols in Southwest Camellia of the present invention is: take dry Southwest Camellia as raw material to carry out solvent extraction, extraction solvent is 50~80% acetone-water solution, solid-liquid ratio (g:ml)= 1:10~40, the extraction temperature is 60~100°C, the extraction time is 1~5h, and the number of extractions is 1~4 times; the extract is concentrated and dried, then dissolved in water and filtered, and the filtrate is absorbed by DM130 macroporous resin, Use 3 to 5 times the amount of resin (ml / g) distilled water to elute impurities, and finally elute with 20 to 60% ethanol solution at a concentration of 12 to 21 times the amount of resin (ml / g), collect the last part of the eluate, and concentrate After drying, the tea polyphenol product of Southwest Camellia can be obtained.

[0027] The following is a detailed description of the extraction of tea polyphenols from Southwest Camellia and the step...

Embodiment 1～4

[0032] The examples in this part are to investigate the influence of the extraction solvent concentration on the yield of tea polyphenols in Southwest Camellia. The specific condition settings and experimental results are shown in Table 1:

[0033] Table 1

[0034]

[0035] The yield of total polyphenols was the highest when the concentration of acetone was 50%, but with the continuous increase of the concentration of acetone, the yield of total polyphenols decreased significantly, which may be due to the good solubility of total polyphenols in 50% acetone aqueous solution and the limited effect on raw materials. The reason for the high swelling coefficient. Therefore, the extraction solvent was selected as 50% acetone-water.

Embodiment 5～9

[0037] The examples in this part are to investigate the influence of extraction time on the yield of tea polyphenols in Southwest Camellia. The specific condition setting and experimental results are shown in Table 2:

[0038] Table 2

[0039]

[0040] The yield of total polyphenols was the highest when the extraction time was 4 hours, and the yield of total polyphenols decreased with the extension of extraction time, so 4 hours was selected as the best extraction time.

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Abstract

The invention provides a preparation method of tea polyphenols in camellia pitardii. According to the method, dried camellia pitardii is used as a raw material, and 50-80% of an acetone-aqueous solution is used as a solvent. The method comprises the following steps of performing reflux extraction, performing concentration and drying on an extracting solution, performing dissolving in water, performing filtration to obtain filtrate, performing adsorption on the filtrate with a DM130 macroporous resin, performing eluting with distilled water for removing impurities, performing eluting with an ethanol solution of which the concentration is 20-60%, collecting an ethanol eluting liquid, and performing concentration and drying so as to obtain tea polyphenol products of the camellia pitardii. According to the preparation method, total polyphenols in the camellia pitardii can be effectively extracted and enriched, the produced total polyphenol is high in purity and good in quality, the composition and the structure of the polyphenol components can be effectively protected, the operation is simple, the cost is low, the used solvent can be recovered, the macroporous resin is regenerable, and the preparation method is suitable for large-scale production.

Description

technical field [0001] The invention relates to a preparation method of active components in natural medicine Southwest Camellia, in particular to a preparation method of tea polyphenols in Southwest Camellia. Background technique [0002] Southwest Camellia (Camellia pitardii), also known as Southwest Red Camellia, is an evergreen evergreen shrub or small tree in Camellia, produced in Hunan, Guangxi, Yunnan, Guizhou, Sichuan and other provinces in my country. Southwest camellia has the effects of promoting blood circulation and stopping bleeding, astringent and diarrhea, detoxification and sores, etc. It is mainly used for irregular menstruation, menorrhagia, intestinal wind bleeding, vomiting blood, acute gastroenteritis, dysentery, prolapse of the anus, leucorrhea, nocturnal emission, rheumatic arthralgia, burns , burns, etc. Tea polyphenols in Southwest Camellia are representative polyphenolic components enriched and purified from Southwest Camellia. Modern pharmacolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/82
CPCA61K36/82A61K2236/333A61K2236/39A61K2236/55
Inventor 于大永宋昱郭秀磊史丽颖
Owner DALIAN UNIV
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