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Natural tissue derived ovarian acellular material and preparation method thereof

A decellularized and tissue-based technology, applied in the field of ovarian decellularized materials derived from natural tissues and its preparation, can solve the problems of reduced survival rate and increased risk of ovarian cancer, and achieve good biocompatibility, good extracellular microenvironment, Good wide range of materials

Active Publication Date: 2017-11-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current treatments include hormone replacement therapy and cryopreservation, which may increase the risk of ovarian cancer, induce malignant cells after autologous retransplantation, and greatly reduce survival

Method used

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  • Natural tissue derived ovarian acellular material and preparation method thereof
  • Natural tissue derived ovarian acellular material and preparation method thereof
  • Natural tissue derived ovarian acellular material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0048] Example 2 Preparation and research of decellularized ovary material

[0049] (1) Material collection: Take healthy ovaries of mature female pigs, one on the left and one on the left, transfer to an environment at 4°C, rinse with sterile saline for 3 times, 20 minutes each time, and remove blood, residual muscle tissue and ligament tissue;

[0050] (2) The ovarian tissue was frozen and thawed at -80°C and 37°C, cycled 3 times, and sliced ​​to 1mm;

[0051] (3) In 1000ml of physiological saline buffer solution containing protease inhibitor, shake at 100rpm at a constant temperature of 37°C for 5 hours; wherein the concentration of physiological saline buffer solution containing protease inhibitor is 1%, and the content of protease inhibitor is 10KIU / ml;

[0052] (4) In 1000 ml of acetone solution with a mass concentration of 50%, degrease with shaking at 100 rpm at a constant temperature of 37° C. for 2 hours;

[0053] (5) In the PBS buffer solution containing 5% Triton ...

Embodiment 3

[0058] Example 3 Preparation and research of decellularized ovary material

[0059] (1) Material collection: Take healthy ovaries of mature female pigs, one on the left and one on the left, transfer to an environment at 4°C, rinse with sterile saline for 3 times, 20 minutes each time, and remove blood, residual muscle tissue, ligaments and other tissues;

[0060] (2) The ovarian tissue was frozen and thawed at -80°C and 37°C, cycled 3 times, and sliced ​​to 2mm;

[0061] (3) In 1000ml of physiological saline buffer solution containing protease inhibitor, shake at 100rpm at a constant temperature of 37°C for 10 hours; wherein the concentration of physiological saline buffer solution containing protease inhibitor is 2%, and the content of protease inhibitor is 10KIU / ml;

[0062] (4) In 1000ml of ethanol solution with a mass concentration of 50%, degrease with shaking at 100rpm at a constant temperature of 37°C for 5 hours;

[0063] (5) In the PBS buffer solution with a mass con...

Embodiment 4

[0067] Example 4 Preparation and research of decellularized ovary material

[0068] Take pig ovary, step (4) degrease in 1000ml of acetone solution with a mass concentration of 10%, shake at a constant temperature of 37°C at 100rpm for 2 hours; for the rest, refer to the method of Example 1 to obtain the decellularized ovary material.

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Abstract

The invention discloses a natural tissue derived ovarian acellular material and a preparation method thereof. The acellular ovarian material is prepared by processing any ovarian tissues of a mammal by virtue of a physiological saline buffer solution containing a protease inhibitor, an organic solvent solution, a PBS buffer solution containing Triton X, a PBS buffer solution containing SDS and a PBS buffer solution containing DNA enzyme. According to the ovarian acellular material provided by the invention, allogeneic or xenogeneic cells, which have immunogenicity, are removed, and meanwhile, original ECM integrity can be reserved; the ovarian acellular material has excellent extracellular microenvironment, biochemical factors, biomechanical properties and the like; and complex tissue structure of a normal ovary can be simulated to the greatest extent.

Description

technical field [0001] The invention mainly relates to the biological field for tissue or organ repair and regeneration, in particular to an ovarian decellularized material derived from natural tissue and a preparation method thereof. Background technique [0002] Premature ovarian failure can be spontaneous or iatrogenic. Current treatments include hormone replacement therapy and cryopreservation techniques, which may increase the risk of ovarian cancer, induce malignant cells after autologous retransplantation, and greatly reduce survival rates. [0003] As an emerging regenerative medicine and tissue engineering technology, decellularization technology retains the natural 3D microstructure and active ingredients in the extracellular matrix collagen, glycosaminoglycan (GAG), while removing immunogenic cells. Cytokines, etc., provide excellent carriers for cell planting, making ovarian regeneration possible. Contents of the invention [0004] The invention provides a su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3633A61L27/3687A61L27/50A61L2430/40
Inventor 林贤丰刘文悦陈蕴缤
Owner ZHEJIANG UNIV
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