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A kind of pretreatment material, its preparation method and application and a kind of antibiotic detection method

A technology for antibiotics and raw materials, applied in the field of antibiotics, can solve the problems of difficulty in simultaneous separation and detection of antibiotics, and achieve the effects of small adsorption effect, strong controllability, and improved accuracy.

Active Publication Date: 2020-07-24
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although great progress has been made in the detection of antibiotics, there are usually great differences between different types of antibiotics, and the simultaneous separation and detection of multiple different types of antibiotics is still difficult.

Method used

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  • A kind of pretreatment material, its preparation method and application and a kind of antibiotic detection method
  • A kind of pretreatment material, its preparation method and application and a kind of antibiotic detection method
  • A kind of pretreatment material, its preparation method and application and a kind of antibiotic detection method

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preparation example Construction

[0020] A method for preparing a pretreatment material provided by an embodiment of the present invention includes:

[0021] The raw materials are taken, preferably, the raw materials include nano activated carbon with a mass ratio of 1 to 2:4 to 6:4 to 6, strong anion exchange packing, and octane solid phase extraction packing. Among them, octane solid-phase extraction filler mainly relies on non-polar carbon bond interaction, which is helpful for the elution and reverse-phase extraction of samples in the non-polar adsorption process, and mainly adsorbs phospholipids and moderately polar compounds. Strong anion exchange packing adsorbs anions, organic acids, nucleic acids, nucleotides and other substances. Nano-activated carbon is mainly physical adsorption, mainly adsorbing gases and pigments. The combination of nano-activated carbon, strong anion exchange packing, and octane solid-phase extraction packing under this ratio makes the material have a small adsorption effect on...

Embodiment 1

[0047] This embodiment provides a kind of pretreatment material and preparation method thereof, comprising:

[0048] Take nano-activated carbon, strong anion exchange packing, and octane solid-phase extraction packing with a mass ratio of 1:4:4.

[0049] Add the above raw materials into a centrifuge tube, and add a certain amount of methanol solvent, wherein the ratio of raw materials to solvent is 5g:25mL. Use a vortex mixer to vortex mix for 1 min, and shake in a constant temperature shaker for 4 h. Centrifuge at 9000r / min for 4min to remove methanol. The centrifuged product was washed once with eluent methanol, and dried at 40° C. for 9 hours to obtain the pretreated material.

Embodiment 2

[0051] This embodiment provides a kind of pretreatment material and preparation method thereof, comprising:

[0052] Take nano-activated carbon, strong anion exchange packing, and octane solid-phase extraction packing with a mass ratio of 2:5:5.

[0053] Add the above raw materials into a centrifuge tube, and add a certain amount of acetonitrile solvent, wherein the ratio of raw materials to solvent is 15g:35mL. Use a vortex mixer to vortex mix for 2 min, and shake in a constant temperature shaking bed for 6 h. Centrifuge at 11000r / min for 6min to remove the acetonitrile solvent. The centrifuged product was washed twice with eluent methanol, and dried at 60° C. for 12 hours to obtain the pretreated material.

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Abstract

Relating to the technical field of antibiotics, the invention provides a pretreatment material, a preparation method and application thereof, and an antibiotic detection method. The preparation method of the pretreatment material includes: mixing raw materials with a solvent, wherein the raw materials include a strong anion exchange packing, an octane solid phase extraction packing and nano activated carbon. The preparation method has simple process and strong controllability, and can achieve large-scale production. The pretreatment material is prepared by the preparation method. The material has good purification effect, can remove a lot of impurities, and is low in economic cost. The antibiotic detection method includes: mixing an extracting solution with a to-be-detected substance, performing centrifugation and taking a first supernatant; mixing the first supernatant with the above pretreatment material, conducting centrifugation, and taking a second supernatant; and detecting the antibiotic in the second supernatant. The method has high recovery rate of antibiotics, and directly achieve selective high throughput determination of a variety of antibiotics, and realizes rapid and simple detection.

Description

technical field [0001] The invention relates to the technical field of antibiotics, and in particular to a pretreatment material, its preparation method and application, and an antibiotic detection method. Background technique [0002] Antibiotics are a class of secondary metabolites with anti-pathogen or other activities produced by microorganisms (including bacteria, fungi, actinomycetes) or higher animals and plants in the course of life, which can interfere with the developmental functions of other living cells chemicals. Antibiotics commonly used in clinical practice include extracts from culture fluid of engineered bacteria and chemically synthesized or semi-synthesized compounds. There are no less than 10,000 known natural antibiotics. The main categories of antibiotics currently used are: sulfonamides, quinolones, penicillins, tetracyclines, macrolides, cephalosporins, polypeptide antibiotics, lincomycins, diterpenes, etc. [0003] In addition to being widely used...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 索德成
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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