Two InDel markers closely linked to yellow-white peel of cucumis sativusL. and application of InDel markers
A yellow-white, chr3indel-fs02 technology, which is applied in the fields of genetic engineering and molecular biology, can solve the problems that there is no InDel marker, weak research on the gene localization of cucumber yellow-white peel, etc., and achieves low experimental cost, simple and clear detection, and improved efficiency. Effect
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Embodiment 1
[0026] 1. Construction of genetic segregation populations
[0027] 1. F 2 Group construction
[0028] YN (female parent), yellow-white peel; the stem is thick and strong, and when the internodes grow to 12-14 nodes, the top is capped with a bunch of female flowers and stops growing, the leaves are green, the fruit is short, the fruit handle is short, and the peel is yellow-white.
[0029] JIN5-508 (male parent), green peel; it is a vine variety with tall plants and long internodes. In each progeny, a plant with a longer fruit stem is selected, and the inbred line with a longer fruit stem is obtained by selfing to the 8th generation when the traits are no longer separated.
[0030] This implementation case uses these two parents to configure the hybrid combination F 1 , F 1 F 2 group. In 551 F 2 In the population, the peel color of cucumber fruits developed 9 days after pollination was measured. The peel color was measured visually and with a colorimeter. Segregation r...
Embodiment 2
[0041] Example 2: Detection of InDel Markers on Cucumber Inbred Lines of Cucumber Peel Color
[0042] (1) Select 20 cucumber inbred lines with known cucumber peel color, and 10 green peels (ACUO57, KP2, AMO22, MM97, OFBAT17, JIN5, EP6411OFBAT07, 649, D8). 10 yellow-white peels (Bai Guifei, CAP15, Dabai, Ocean White Cucumber, CAP14, CAP11, YN, Pingwang Milkmelon, Baixiu, FRANCH).
[0043] (2) Genomic DNA of the above-mentioned 20 varieties and their parents were extracted respectively, and PCR amplification was performed using Chr3InDel-fs01 and Chr3InDel-fs02 markers. The results of ChrINdel-fs01 in 10 green peel cucumber inbred lines and 10 yellow-white peel cucumber inbred lines showed that the genotype was consistent with the phenotype. (Such as Figure 5 ) ChrINdel-fs02 in 10 green peel cucumber inbred lines and 10 yellow-white peel cucumber inbred lines, the genotype is consistent with the phenotype. (Such as Figure 6 )
[0044] (3) The results of agarose gel electr...
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