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Rapid detection kit for dog mature echinococcosis antigen and use method of rapid detection kit

A detection kit and technology for fecal hydatid disease, which are applied in biological tests, measuring devices, material inspection products, etc., can solve the problems of long detection time, time-consuming and laborious, and achieve simple detection work, shortened test time, and high sensitivity. Effect

Inactive Publication Date: 2017-11-28
SHANGHAI NEW JIEER CLEANING PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention provides a rapid detection kit (colloidal gold method) for canine fecal echinococcosis antigen and its use method, which overcomes the above-mentioned deficiencies in the prior art, and can effectively solve the existing process of detecting Echinococcus canis infection The problems of long detection time, time-consuming and labor-intensive, and the need for special test auxiliary equipment

Method used

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  • Rapid detection kit for dog mature echinococcosis antigen and use method of rapid detection kit
  • Rapid detection kit for dog mature echinococcosis antigen and use method of rapid detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Such as Figures 1 to 2 As shown, the canine fecal echinococcosis antigen rapid detection kit (colloidal gold method), including sample pad 1, gold standard pad 2, nitrocellulose membrane 3, water-absorbing pad 4 and base plate 5, on the left top of base plate 5 A sample pad 1 is fixed, a gold standard pad 2 is fixed between the right part of the sample pad 1 and the bottom plate 5, a water-absorbing pad 4 is fixed on the right top of the bottom plate 5, and a gold standard pad 2 and the water-absorbing pad 4 are fixed. There is a nitrocellulose membrane 3, the height of the nitrocellulose membrane 3 is lower than the height of the right part of the gold standard pad 2 and the height of the left part of the water-absorbing pad 4, and a detection line 6 is longitudinally drawn on the upper end surface of the nitrocellulose membrane 3. The upper end surface of the nitrocellulose membrane 3 on the right side of the detection line 6 is longitudinally marked with a quality c...

Embodiment 2

[0055] as attached Figures 1 to 2 As shown, the canine fecal echinococcosis antigen rapid detection kit (colloidal gold method), including sample pad 1, gold standard pad 2, nitrocellulose membrane 3, water-absorbing pad 4 and base plate 5, on the left top of base plate 5 A sample pad 1 is fixed, a gold standard pad 2 is fixed between the right part of the sample pad 1 and the bottom plate 5, a water-absorbing pad 4 is fixed on the right top of the bottom plate 5, and a gold standard pad 2 and the water-absorbing pad 4 are fixed. There is a nitrocellulose membrane 3, the height of the nitrocellulose membrane 3 is lower than the height of the right part of the gold standard pad 2 and the height of the left part of the water-absorbing pad 4, and a detection line 6 is longitudinally drawn on the upper end surface of the nitrocellulose membrane 3. The upper end surface of the nitrocellulose membrane 3 on the right side of the detection line 6 is longitudinally marked with a quali...

Embodiment 3

[0068] The difference from the above-mentioned embodiments is that the nitrocellulose membrane 3 is a nitrocellulose membrane activated by a carbodiimide hydrochloride solution; or / and, the sample pad 1 is made of Bovine albumin, 1% sucrose, 0.1% Tween-20 and 0.02% sodium azide phosphate buffer pretreated sample pad 1, the phosphate buffer has a molar concentration of 0.01 mol / L, Phosphate buffer with a pH value of 7.4; or / and, gold standard pad 2 is a gold standard pad after pretreatment with a phosphate buffer containing 1% by weight of defatted casein, and the phosphate buffer is mol Phosphate buffer solution with a concentration of 0.05 mol / L and a pH value of 7.2.

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Abstract

The invention relates to a rapid detection kit for a dog mature echinococcosis antigen and a use method of the rapid detection kit. The rapid detection kit comprises a sample pad, a gold mark pad, a nitrocellulose membrane, a water absorption pad and a base plate, wherein the sample pad is fixed at the top end of the left part of the base plate, the gold mark pad is fixed between the right part of the sample pad and the base plate, the water absorption pad is fixed at the top end of the right part of the base plate, and the nitrocellulose membrane is fixed between the gold mark pad and the water absorption pad. The rapid detection kit for the dog mature echinococcosis antigen (colloidal gold method) has relatively high sensitivity, specificity and stability, can be carried along, can be used for carrying out field testing at any time anywhere and does not require special testing auxiliary equipment, so that the testing time is shortened, and the testing efficiency is improved; and besides, the rapid detection kit is used for testing dog mature, so that the injury caused to dogs is avoided, and the detection work is relatively simple and convenient.

Description

technical field [0001] The invention relates to the technical field of canine Echinococcus granulosus detection technology, in particular to a canine fecal echinococcosis antigen rapid detection kit (colloidal gold method) and a use method thereof. Background technique [0002] Echinococcus granulosus is the causative agent of cystic echinococcosis in humans and domestic animals (sheep, cattle, camels, etc.). Echinococcus granulosus has two life stages, one of which is the adult stage, the adult stage is hermaphrodite, parasitic in the small intestine of domestic dogs, fixed on the small intestinal mucosa by the head hooks and suckers on the first segment, through autologous or heterologous mating Complete the fertilization process. Adults consist of four segments: cephalic, cervical, adult and gestational. After the fertilized eggs develop and mature in the uterus of the gestational segment, the gestational segment falls off and disintegrates, and the eggs are excreted wi...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/53
CPCG01N33/5308G01N33/558G01N2333/43543
Inventor 朱劼远李宝良焦伟杨安姚霞范继康
Owner SHANGHAI NEW JIEER CLEANING PRODS
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