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Method for preparing herbicide by using starch wastewater

A starch wastewater, herbicide technology, applied in herbicides and algicides, botanical equipment and methods, microorganism-based methods, etc. The effect of promoting nutrient utilization, facilitating large-scale production, and improving metabolic capacity

Active Publication Date: 2017-12-22
来安县永阳知识产权运营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among starch wastewater treatment methods, microbial method is the most popular method, but at present, its main method is to produce single-cell protein feed, which is relatively simple.
[0003] At present, weeding in agriculture is mostly done chemically, but the result of this method is to make weeds resistant to pesticides, and at the cost of environmental pollution, it is also harmful to humans and animals.
The original Streptomyces propurpuratus (Streptomyces propurpuratus) whose strain number is ACCC 41492, its metabolites have insecticidal and herbicidal activities, but its growth on Gaoshi No. Weaker, it is not only expensive to directly prepare biological herbicides, but also has extremely low efficiency, making it difficult to use on a large scale
No report on the preparation of herbicides from starch wastewater has been found so far

Method used

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  • Method for preparing herbicide by using starch wastewater
  • Method for preparing herbicide by using starch wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of method utilizing starch waste water to prepare herbicide, comprises the following steps:

[0029] 1) Wastewater conditioning: mix the fish scale powder and vermicompost evenly in a weight ratio of 1:5, and let it stand for 24 hours under indoor ventilation to obtain the first mixture, and mix the first mixture with starch wastewater in a weight ratio of 1:8 Put it in a stirring tank, stir it for 30 minutes every 3.5 hours at a speed of 10 r / min under indoor ventilation, and adjust the pH to 7.0 after 48 hours to obtain the second mixture;

[0030] 2) Seed preparation: sterilize and cool the second mixture and inoculate the original Streptomyces crimson, the inoculation concentration of the original Streptomyces crimson is inoculated with 10 per milliliter of the sterilized second mixture. 6 1 spore, cultured in a shaker flask at 28-30°C at 150r / min for 36h to obtain liquid seeds. The strain number of the original Streptomyces crimson rubrum is ACCC41492;

[0...

Embodiment 2

[0038] A kind of method utilizing starch waste water to prepare herbicide, comprises the following steps:

[0039] 1) Wastewater conditioning: mix the fish scale powder and vermicompost evenly in a weight ratio of 1:5, and leave it to stand for 24 hours under indoor ventilation to obtain the first mixture, and mix the first mixture with starch wastewater in a weight ratio of 1:10 Place it in a stirring tank, and stir it for 30 minutes every 3.5 hours at a speed of 10 r / min under indoor ventilation, and adjust the pH to 7.4 after 48 hours to obtain the second mixture;

[0040] 2) Seed preparation: sterilize and cool the second mixture and inoculate the original Streptomyces crimson, the inoculation concentration of the original Streptomyces crimson is inoculated with 10 per milliliter of the sterilized second mixture. 7 1 spore, cultured in a shaker flask at 28-30°C at 150r / min for 36h to obtain liquid seeds. The strain number of the original Streptomyces crimson rubrum is ACCC...

Embodiment 3

[0048] A kind of method utilizing starch waste water to prepare herbicide, comprises the following steps:

[0049] 1) Wastewater conditioning: mix the fish scale powder and vermicompost evenly in a weight ratio of 1:5, and leave it to stand for 24 hours under indoor ventilation to obtain the first mixture, and mix the first mixture with starch wastewater in a weight ratio of 1:9 Place it in a stirring tank, and stir it for 30 minutes every 3.5 hours at a speed of 10 r / min under indoor ventilation, and adjust the pH to 7.2 after 48 hours to obtain the second mixture;

[0050] 2) Seed preparation: sterilize and cool the second mixture and inoculate the original Streptomyces crimson, the inoculation concentration of the original Streptomyces crimson is 5×10 per milliliter of the sterilized second mixture. 6 1 spore, cultured in a shaker flask at 28-30°C at 150r / min for 36h to obtain liquid seeds. The strain number of the original Streptomyces crimson rubrum is ACCC41492;

[0051...

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Abstract

The invention discloses a method for preparing herbicide by using starch wastewater. The method comprises the following steps: (1) uniformly mixing fish scale powder with earthworm cast according to a weight ratio of 1 to 5, leaving the mixture to stand for 24 hours, thus obtaining a first mixture, mixing the first mixture with the starch wastewater according to a weight ratio of 1 to (8 to 10), performing stirring treatment at a stirring rate of 10 r / min for 30 minutes every 3.5 hours, and adjusting the pH after 48 hours, thus obtaining a second mixture; (2) performing sterilization cooling on the second mixture, inoculating original magenta streptomyces, and performing shake-flask culture, thus obtaining liquid seeds; (3) putting the second mixture into a first fermentation tank, performing sterilization cooling to room temperature, inoculating 10 to 15 percent of the liquid seeds, and feeding air for aerobic culture, thus obtaining a fermentation product; (4) filtering the fermentation product to obtain filtrate, adjusting the pH, and concentrating the filtrate by a supercentrifugal process, thus obtaining the herbicide. The herbicide disclosed by the invention is good in weed control effect, safe and harmless to the environment and low in cost.

Description

technical field [0001] The invention relates to the technical field of recycling starch wastewater. More specifically, the present invention relates to a method for preparing herbicides from starch wastewater. Background technique [0002] The starch industry is an industry that uses corn, potatoes, wheat, rice and other agricultural products as raw materials to produce starch or starch deep-processing products (starch sugar, glucose, starch derivatives, etc.). In the production process of the starch industry, the water demand is very large, and the wastewater discharge is also large, and the wastewater is high-concentration organic wastewater containing a large amount of starch, protein, sugar, fat and other organic substances. If it is not treated and directly discharged into the water body, It will cause hypoxia in the water body, suffocate aquatic animals, and bring great harm to the environment. At present, my country's annual production of starch is more than 3 milli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P13/00C12N1/20C12R1/465C12R1/125
CPCA01N63/10C12N1/20
Inventor 刘钰馨
Owner 来安县永阳知识产权运营有限公司
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