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Preparation method of bacteriostatic and wound restoration promoting chitosan hydrogel dressing

A technology of wound repair and chitosan, which is applied in the application field of biomedical materials, can solve the problems of general antibacterial performance and reduction

Active Publication Date: 2017-12-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country carried out the research and development of chitosan dressings in the 1980s. Compared with traditional dressings such as vaseline gauze, they are better in wound protection and healing promotion, but their antibacterial properties are average, and their clinical use has gradually decreased since then.

Method used

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  • Preparation method of bacteriostatic and wound restoration promoting chitosan hydrogel dressing
  • Preparation method of bacteriostatic and wound restoration promoting chitosan hydrogel dressing
  • Preparation method of bacteriostatic and wound restoration promoting chitosan hydrogel dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] First, 2.5 g of molecular weight 1 × 10 5 Da, chitosan with a degree of deacetylation of 70% is dissolved in 250mL of 1% (v / v) acetic acid aqueous solution, and then 125mL of 1,2-dichloroethane and 0.94g of cellulose acetate are added at 35°C Stir at 1000rpm for 1 hour to emulsify under the conditions, add 12.5mL glutaraldehyde with a volume ratio concentration of 1% (v / v) and continue stirring for 1 hour to fix the microspheres, then use 387.5mL saturated sodium chloride solution to break the emulsion, centrifuge, Wash to obtain chitosan microspheres; 20mg chitosan microspheres are uniformly dispersed in 250mL mass volume ratio concentration of chitosan aqueous solution of 1% (w / v), at 20 ℃ with 12.5mL volume ratio concentration 1% (v / v) glutaraldehyde cross-linked for 1 hour, then soaked in deionized water to wash off excess cross-linking agent, and removed 10% of water in an oven to obtain a chitosan hydrogel dressing.

Embodiment 2

[0017] First, 3 g of molecular weight 1 × 10 4 Da, the chitosan that deacetylation degree is 85% is dissolved in 250mL volume ratio concentration and is the acetic acid aqueous solution of 1% (v / v), then adds 150mL ethyl acetate and 5g cellulose acetate butyrate, under 45 ℃ of conditions Stir at 750rpm for 1.5 hours to emulsify, add 25mL glutaraldehyde with a volume ratio concentration of 1% (v / v) and continue stirring for 1.5 hours to fix the microspheres, then use 820mL saturated sodium chloride solution to break the emulsion, centrifuge and wash to obtain chitosan Sugar microspheres; 15mg chitosan microspheres are uniformly dispersed in 250mL mass volume ratio concentration of chitosan aqueous solution of 2% (w / v), at 15 ℃ with 37.5mL volume ratio concentration of 1% (v / v) glutaraldehyde cross-linked for 2 hours, then soaked with deionized water, washed off excess cross-linking agent, and removed 20% of moisture in an oven to obtain a chitosan hydrogel dressing.

Embodiment 3

[0019] First, 5g of molecular weight is 3×10 5 Da, chitosan with a deacetylation degree of 99% is dissolved in 250mL volume ratio concentration of 3% (v / v) acetic acid aqueous solution, then 250mL cyclohexanone and 12.5g cellulose acetate propionate are added, at 40°C Stir at 1000rpm for 1 hour to emulsify, add 37.5mL of glutaraldehyde with a volume ratio concentration of 1% (v / v) and continue to stir for 2 hours to fix the microspheres, then use 1000mL saturated sodium chloride solution to break the emulsion, centrifuge and wash to obtain Chitosan microspheres: 10mg chitosan microspheres are uniformly dispersed in 250mL mass volume ratio concentration of 4% (w / v) chitosan aqueous solution, and 12.5mL volume ratio concentration is 1% under the condition of 30 ℃. (v / v) glutaraldehyde was cross-linked for 2 hours, then soaked with deionized water to wash away excess cross-linking agent, and removed 15% of moisture in an oven to obtain a chitosan hydrogel dressing.

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Abstract

The invention belongs to the field of biomedical material application, and particularly relates to a preparation method of a bacteriostatic and wound restoration promoting chitosan hydrogel dressing. According to the preparation method, firstly, chitosan is prepared into microspheres in a dispersion medium by a Pickering emulsion process; after glutaraldehyde fixation, the microspheres are uniformly dispersed into a chitosan water solution; then, glutaraldehyde crosslinking agents are used for preparing hydrogel; and through soaking and flushing by deionized water, excessive cross linking agents and certain moisture are removed to obtain the chitosan hydrogel dressing. The dressing has soft textures; the inside has the uniform three-dimensional hole structures; wound percolate and secreta can be sucked away; the chitosan microspheres loaded by the hydrogel have high-density positive charges; the bacteria growth can be inhibited; the additional addition of bacteriostatic medicine is not needed; the wound restoration can also be promoted; and the dressing can be applied to the restoration of wound and the protection of various kinds of skin wound surfaces such as burn injury, mechanical trauma, diabetic foot and pressure sores.

Description

technical field [0001] The invention belongs to the application field of biomedical materials, and in particular relates to a preparation method of a chitosan hydrogel dressing capable of inhibiting bacteria and promoting wound repair. Background technique [0002] In daily life, people's skin is often damaged by burns, scalds and mechanical trauma, as well as skin damage caused by diabetic feet and pressure sores. The repair process after skin damage is generally divided into four stages: coagulation stage-inflammation stage-proliferation stage-remodeling stage. It can also cause tissue necrosis and other problems. Thus, promoting wound repair and effectively preventing wound infection is a challenging problem. In the course of treatment, early detection and rapid, appropriate and effective sterilization and antibacterial are particularly important for improving the effect of wound repair. Especially in today's increasingly serious antibiotic resistance, it is also neces...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0066A61L26/008A61L26/0085A61L26/0095A61L2300/236A61L2300/404A61L2300/412A61L2300/622A61L26/0023C08L5/08
Inventor 陈荆晓徐政吴婧陈敬华
Owner JIANGNAN UNIV
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