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Efficient extraction method of cordyceps militaris polysaccharide

A technology for polysaccharide extraction and grass seed, which is applied in the field of efficient polysaccharide extraction from fruiting bodies of Cordyceps militaris, can solve the problems of high extraction conditions and extraction environment requirements, inflammable and explosive, and activated carbon decolorization time is long, and achieves economical extraction, separation and purification process. The effect of fast mass transfer and equilibration, easy continuous operation

Inactive Publication Date: 2017-12-22
成都菱祐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Deproteinization involves low-toxic organic reagents and second-class precursor organic reagents, and the decolorization efficiency is low, repeated extractions are required, the process is cumbersome, the loss is large, and the problem of easy organic reagent residues and environmental hazards Wait
[0005] 2. Hydrogen peroxide is a strong oxidizing agent in the hydrogen peroxide decolorization method, and there may be a risk of destroying some active substances in polysaccharides
[0006] 3. The decolorization time of activated carbon is long, the decolorization efficiency is low, multiple decolorization is required, the decolorization is cumbersome, and it also has a certain adsorption effect on polysaccharides, and the loss of polysaccharides is large
[0007] 4. Organic reagents such as petroleum ether and ether have a low boiling point and are flammable and explosive. They have high requirements for extraction conditions and extraction environments, and there are certain risks

Method used

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  • Efficient extraction method of cordyceps militaris polysaccharide
  • Efficient extraction method of cordyceps militaris polysaccharide
  • Efficient extraction method of cordyceps militaris polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] refer to figure 1 , a kind of high-efficiency extracting method of fruiting body polysaccharide of Cordyceps militaris, comprises the following steps successively:

[0048] a. Take 10 parts of Cordyceps militaris fruiting body according to weight, and use ultrasonic-assisted two-phase extraction method to extract the fruiting body of Cordyceps militaris; ultrasonic-assisted two-phase extraction method is: use ammonium sulfate-polyethylene glycol 6000 two-phase system The fruiting body of Cordyceps militaris was subjected to ultrasonic-assisted solid-liquid extraction for 20 minutes.

[0049] b. Suction filtration twice and discard the filtrate, and perform ultrasonic water extraction on the filter residue; the specific steps of ultrasonic water extraction are: add 10 parts (by weight) of water to the filter residue, and perform ultrasonic water extraction for 20 minutes.

[0050] c, suction filtration and combined filtrate;

[0051] d. Concentrate the filtrate to a se...

Embodiment 2

[0054] An efficient method for extracting polysaccharides from the fruiting bodies of Cordyceps militaris, comprising the following steps in sequence:

[0055] a. Take 15 parts of Cordyceps militaris fruiting bodies according to weight, and use ultrasonic-assisted two-phase extraction method to extract the fruiting bodies of Cordyceps militaris; ultrasonic-assisted two-phase extraction method is: use ammonium sulfate-polyethylene glycol 6000 two-phase system The fruiting body of Cordyceps militaris was subjected to ultrasonic-assisted solid-liquid extraction for 30 minutes.

[0056] b. Suction filtration 3 times and discard the filtrate, carry out ultrasonic water extraction to the filter residue; the specific steps of ultrasonic water extraction are: add 20 parts (by weight) of water to the filter residue, and perform ultrasonic extraction for 30 minutes.

[0057] c, suction filtration and combined filtrate;

[0058] d. Concentrate the filtrate to a set volume, and then use ...

Embodiment 3

[0061] An efficient method for extracting polysaccharides from the fruiting bodies of Cordyceps militaris, comprising the following steps in sequence:

[0062] a. Take 30 parts of Cordyceps militaris fruiting bodies according to weight, and use ultrasonic-assisted two-phase extraction method to extract the fruiting bodies of Cordyceps militaris; ultrasonic-assisted two-phase extraction method is: use ammonium sulfate-polyethylene glycol 6000 two-phase system The fruiting body of Cordyceps militaris was subjected to ultrasonic-assisted solid-liquid extraction for 40 minutes.

[0063]b. Suction filtration 5 times and discard the filtrate, carry out ultrasonic water extraction to the filter residue; the specific steps of ultrasonic water extraction are: add 30 parts (parts by weight) of water to the filter residue, and perform ultrasonic extraction for 40 minutes.

[0064] c, suction filtration and combined filtrate;

[0065] d. Concentrate the filtrate to a set volume, and then...

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Abstract

The invention provides an efficient extraction method of cordyceps militaris polysaccharide. The method comprises the following steps sequentially: a, taking cordyceps militaris, and performing ultrasonic solid-liquid extraction on cordyceps militaris by adopting an ultrasonic alcohol extraction method; b, performing suction filtration, discarding filtrate, and performing ultrasonic water extraction on filter residues; c, performing suction filtration, and combining the filtrate; d, concentrating the filtrate till a set volume is achieved, and performing alcohol precipitation with ethyl alcohol, so as to obtain alcohol precipitation fluid; e, centrifuging the alcohol precipitation fluid, discarding supernate, and performing drying and precipitation, so as to obtain cordyceps militaris polysaccharide. The method maintains the biological activity of a polysaccharide ingredient contained in the cordyceps militaris. Inactivation or degeneration of the biologically active substance cannot be caused. Extraction reagents adopted in the method are nontoxic reagents, safe and environmentally friendly. The method can achieve the effects of extraction, decoloration and deproteinization during extraction, thereby reducing processing steps of single decoloration and deproteinization, and simplifying the technology.

Description

technical field [0001] The invention relates to an improved method for extracting polysaccharides from the fruiting bodies of Cordyceps militaris with high efficiency, environmental protection and greenness, in particular to an efficient method for extracting polysaccharides from the fruiting bodies of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is a well-known edible and medicinal fungus. Cordyceps polysaccharides are its important bioactive components. Cordyceps polysaccharides have antioxidant activity, enhance immunity, anti-tumor, and lower blood sugar and blood lipids. [0003] The extraction of existing Cordyceps militaris polysaccharides includes alcohol extraction and water extraction, and methods such as decoction, Soxhlet extraction, microwave-assisted extraction and ultrasonic extraction, but these extraction methods will simultaneously extract A large number of impurities such as pigments and water-s...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陈露李燕雏彭毅苏超张楠周旭峰
Owner 成都菱祐生物科技有限公司