Method for enriching 2-diabetes mellitus target inhibitor from liquorice residue and medical application

A type 2 diabetes, inhibitor technology, applied in pharmaceutical formulations, non-central analgesics, applications, etc., can solve the problem that the purity of total flavonoids of licorice cannot be guaranteed to be greater than or equal to 80%, and the content and activity of monomeric flavonoids are not measured. The data of licorice root is not clear, the composition of total flavonoid monomers of licorice is not clear, etc., to achieve the effect of protecting liver damage, simple and easy process operation, and inhibiting tumor growth.

Inactive Publication Date: 2017-12-26
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In summary, when extracting and purifying the total flavonoids in licorice residue, most of them use other organic reagents except ethanol, which is not good for the environment, the content cannot be determined, and the purity of total flavonoids in licorice cannot be guaranteed to be greater than or equal to 80%, the important thing is that the composition of the monomer components of total flavonoids in licorice is not clear, and there is no data on the content and activity determination of monomer flavonoids, and there is no expression of specific medical use

Method used

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  • Method for enriching 2-diabetes mellitus target inhibitor from liquorice residue and medical application
  • Method for enriching 2-diabetes mellitus target inhibitor from liquorice residue and medical application
  • Method for enriching 2-diabetes mellitus target inhibitor from liquorice residue and medical application

Examples

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Effect test

Embodiment 1

[0036] Example 1: Take an appropriate amount of licorice residue, add ethanol with a concentration of 70%, the ratio of solid to liquid is 1:30, extract at 90°C for 2 hours, extract 3 times, combine the extracts, and recover ethanol. Make the concentration of the licorice extract solution 4.0mg / mL, then pass through the macroporous resin: polyamide = 1:1 adsorption resin column at a flow rate of 1BV / h, stay for 12h after loading the sample, make it fully adsorbed, and use 5BV 50% Ethanol removes impurities at a flow rate of 2BV / h, then elutes 15 retention volumes with 80% ethanol at a flow rate of 3BV / h, recovers the eluate, and dries to obtain LRTF with a purity of 80% of the total extract content. Radix Glycyrrhizae The content of ketone A accounts for 10% of the total extract content. Using HPLC-MS to conduct ion fragment analysis to speculate its chemical composition, use various chromatographic separation and purification, and conduct physical and chemical constants and s...

Embodiment 2

[0038] Take an appropriate amount of licorice residue, add ethanol with a concentration of 70%, the ratio of solid to liquid is 1:30, extract at 90°C for 2 hours, extract 3 times, combine the extracts, and recover ethanol. Make the concentration of the licorice extract solution 4.0mg / mL, then pass through the macroporous resin:polyamide=5:1 adsorption resin column at a flow rate of 1BV / h, stay for 12h after loading the sample, make it fully adsorbed, and use 5BV 50% Ethanol removes impurities at a flow rate of 2BV / h, then elutes 15 retention volumes with 80% ethanol at a flow rate of 3BV / h, reclaims the eluate, and dries to obtain LRTF content that accounts for 85% of the total extract content. Radix Glycyrrhizae The content of ketone A accounts for 12% of the total extract content. Using HPLC-MS to conduct ion fragment analysis to speculate its chemical composition, use various chromatographic separation and purification, and conduct physical and chemical constants and spectr...

Embodiment 3

[0040] Take an appropriate amount of licorice residue, add ethanol with a concentration of 70%, the ratio of solid to liquid is 1:30, extract at 90°C for 2 hours, extract 3 times, combine the extracts, and recover ethanol. Make the concentration of the licorice extract solution 4.0mg / mL, then pass through the macroporous resin:polyamide=10:1 adsorption resin column at a flow rate of 1BV / h, stay for 12h after loading the sample, make it fully adsorbed, and use 5BV 50% Ethanol removes impurities at a flow rate of 2BV / h, then elutes 15 retention volumes with 80% ethanol at a flow rate of 3BV / h, recovers the eluate, and dries to obtain 90% of the total extract content with LRTF content. The content of ketone A accounts for 18% of the total extract content. Using HPLC-MS to conduct ion fragment analysis to speculate its chemical composition, use various chromatographic separation and purification, and carry out physical and chemical constants and spectral identification to clarify ...

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Abstract

The invention belongs to the technical field of medicines and provides a method for adsorbing, purifying and acquiring liquorice residue total flavone with clear component, clear structure, high active substance content and high activity from liquorice residue after extracting glycyrrhizic acid by using macroporous resin as a main material (above 50%), polyamide as an auxiliary material and a compound adsorbing material prepared from one or two MCI mixed at different ratio according to a compound adsorbing purifying method and a medical application thereof at the aspects of blood sugar regulation, liver injury protection, blood lipids regulation, tumor growth suppression, and the like. According to the invention, various adsorbing materials are reasonably combined and designed and the adsorbing purifying technical operation is simple and feasible; the solvent use is safe, environment-friendly and economical; and the liquorice residue total flavone has an obvious restraining effect for two important targets, namely, alpha-glucosidase and PTP1B for preventing and controlling 2-diabetes mellitus, and especially, the restraining effect strength for alpha-glucosidase is at least 10 times higher than that of clinic hypoglycemic drug acarbose.

Description

Technical field: [0001] The invention relates to the field of medical technology, and relates to a method for enriching high-purity type 2 diabetes target inhibitors from licorice residue after extraction of glycyrrhizic acid by using a composite adsorption method and a medical application, in particular to a kind of licorice from Ural A method for extracting licorice total flavonoids from licorice residue after extracting glycyrrhizic acid from different kinds of licorice, such as licorice glabra, licorice glabra, and licorice bulging, and its application in the preparation of health food, food additive, medicine or pharmaceutical raw material. Background technique: [0002] Licorice, also known as Guolao, Lingtong, Sweetgrass, Meicao, Honeygrass, etc., is a perennial herb of the Fabaceae Papilionaceae subfamily, and is a commonly used traditional Chinese medicine. So far, more than 300 kinds of flavonoids and their derivatives have been isolated from the roots, stems and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/484A23L33/105A61P3/10A61P3/06A61P35/00A61P1/16A61P29/00
CPCA23L33/105A23V2002/00A61K36/484A61K2236/333A61K2236/39A61K2236/55A23V2200/328A23V2200/3262A23V2200/32A23V2200/30A23V2250/2116A23V2300/14A23V2200/308
Inventor 赵余庆肖婷郭正红
Owner SHENYANG PHARMA UNIVERSITY
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