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Method for dissolving and separating silkworm pupa proteins from silkworm pupas by virtue of degradable ionic liquid

An ionic liquid and silkworm chrysalis protein technology is applied in the field of low-fat silkworm chrysalis protein extraction and separation, high purity, and the use of degradable ionic liquid to dissolve and separate silkworm chrysalis protein. The effect of good effect, short dissolution time and simple process flow

Inactive Publication Date: 2017-12-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to overcome the disadvantages of high fat content of silkworm chrysalis protein obtained by the traditional method and will cause acid-base pollution, etc., and provides a method for extracting and separating protein in silkworm chrysalis by using degradable ionic liquid as a solvent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take by weighing 0.2503g particle diameter and be the defatted silkworm chrysalis powder after drying of 200 meshes, mix evenly with 5g of choline acetate ionic liquid with a water content of 0.47%, stir at 90°C for 4 hours, stop the reaction, and centrifuge to obtain silkworm chrysalis protein ionic liquid solution, Add about 50ml of ethanol to the solution as a regeneration agent, stir, stand still, and centrifuge to obtain a precipitate of regenerated silkworm chrysalis protein, wash with ethanol until no ionic liquid remains, and place it in a freeze dryer to dry to obtain a light gray solid, which is the product silkworm chrysalis protein. The yield of silkworm chrysalis protein is 58.91%, the protein content of the obtained silkworm chrysalis protein is 81.36%, and the fat content is 0.18%.

Embodiment 2

[0023] Weigh 0.2508g of dried degreased silkworm chrysalis powder with a particle size of 200 mesh, mix evenly with 5g of choline propionate ionic liquid with a water content of 0.48%, stir at 90°C for 4 hours, stop the reaction, and centrifuge to obtain silkworm chrysalis protein ionic liquid solution , add about 50ml of ethanol to the solution as a regenerating agent, stir, stand still, and centrifuge to obtain a precipitate of regenerated silkworm chrysalis protein, wash with ethanol until no ionic liquid remains, place it in a freeze dryer to dry, and obtain a light gray solid, which is the product Silkworm chrysalis protein. The yield of silkworm chrysalis protein is 50.28%, the protein content of the obtained silkworm chrysalis protein is 79.66%, and the fat content is 0.21%.

Embodiment 3

[0025] Weigh 0.2511g of dried degreased silkworm chrysalis powder with a particle size of 200 meshes, mix evenly with 5g of choline butyrate ionic liquid with a water content of 0.46%, stir at 90°C for 4 hours, stop the reaction, and centrifuge to obtain silkworm chrysalis protein ionic liquid solution , add about 50ml of ethanol to the solution as a regenerating agent, stir, stand still, and centrifuge to obtain a precipitate of regenerated silkworm chrysalis protein, wash with ethanol until no ionic liquid remains, place it in a freeze dryer to dry, and obtain a light gray solid, which is the product Silkworm chrysalis protein. The yield of silkworm chrysalis protein is 39.72%, the protein content of the obtained silkworm chrysalis protein is 81.36%, and the fat content is 0.33%.

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Abstract

The invention belongs to the technical field of separation extraction of natural polymers, relates to a method for extracting and separating high-purity and low-fat silkworm pupa proteins and particularly relates to a method for extracting and separating the silkworm pupa proteins from by virtue of degradable ionic liquid. The method specifically comprises the following steps: (1) raw material pretreatment: drying and crushing fresh silkworm pupas, dried silkworm pupas or degreased dried silkworm pupas, and sieving, so as to obtain silkworm pupa powder; (2) dissolving: uniformly mixing the silkworm pupa powder with the degradable ionic liquid, heating, stirring to adequately dissolve the silkworm pupa powder, and carrying out centrifugal separation, so as to obtain a silkworm pupa protein ionic liquid solution; (3) regeneration: mixing the silkworm pupa protein ionic liquid solution with a regenerant, stirring, standing, carrying out centrifugation, so as to obtain regenerated silkworm pupa protein precipitates; and (4) drying: carrying out freeze drying so as to obtain regenerated silkworm pupa proteins. The ionic liquid adopted in the method has good biocompatibility and biodegradability and is non-corrosive and pollution-free, the damage level of the proteins is low, and the obtained product is high in purity.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of natural polymers, and relates to a method for extracting and separating silkworm chrysalis protein with high purity and low fat, in particular to a method for dissolving and separating silkworm chrysalis protein by utilizing degradable ionic liquid. Background technique [0002] my country's mulberry industry has a long history. As an accessory of sericulture and silk reeling, the current annual output of silkworm cocoons reaches 7 billion tons. With the development of science and technology, it can be known by modern analysis methods that the protein content in fresh pupae accounts for 45% to 50% of the mass of pupae, and the protein content in waste silkworm pupae dregs after oil extraction can reach more than 70%, which shows that its protein content is very rich. After in-depth analysis, it can be seen that there are many kinds of amino acids in silkworm chrysalis protein,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 吕兴梅张博康艳红冯咪徐俊丽贾彩张锁江
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI