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Chemically constructed multifunctional non-viral gene conveying carrier polymer based on thiolactone

A technology of polymers and compounds, applied in gene therapy, genetic material components, and other methods of inserting foreign genetic materials, etc., to achieve the effects of wide application prospects, low cytotoxicity, and high transfection efficiency

Active Publication Date: 2017-12-26
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, safe and efficient delivery of nucleic acids to target sites remains a major challenge in the application of gene therapy

Method used

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  • Chemically constructed multifunctional non-viral gene conveying carrier polymer based on thiolactone
  • Chemically constructed multifunctional non-viral gene conveying carrier polymer based on thiolactone
  • Chemically constructed multifunctional non-viral gene conveying carrier polymer based on thiolactone

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Experimental program
Comparison scheme
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Embodiment 1

[0047] The synthetic method of embodiment 1 compound 1, 2 and 3

[0048] Through the amidation reaction between D, L-homocysteine ​​thiolactone hydrochloride and n-valeric acid, n-nonanoic acid and perfluorobutyric acid, thiolactone compounds with different types of hydrophobic moieties were synthesized (respectively Recorded as: 1,2,3). Taking the synthesis of compound 1 as an example, at first, 100 mL of dichloromethane (DCM) containing D, L-homocysteine ​​sulfate lactone hydrochloride (6.12g, 40mmol), triethylamine (7mL, 50mmol) at room temperature Under stirring, then add n-nonanoic acid (7.0g, 45mmol), dicyclohexylcarbodiimide (DCC) (9.2g, 45mmol) and dimethylaminopyridine (DMAP) (550mg, 4.5mmol), continue the reaction mixture Stir at room temperature for 24 hours. Next, the reactant was suction-filtered under reduced pressure to remove the insoluble residue, washed with ethyl acetate and filtered several times. After the solvent was evaporated under reduced pressure, ...

Embodiment 2

[0049] The synthetic method of embodiment 2 polyacryloxyethyl methacrylate (PAOEMA)

[0050] Such as Figure 5 As shown, PAOEMA is synthesized by post-modification of poly(2-hydroxyethyl methacrylate) (PHEMA) with acryloyl chloride. First, we synthesized PHEMA by reversible addition-fragmentation chain transfer (RAFT) polymerization. Specifically, the monomer 2-hydroxyethyl methacrylate (HEMA) (5.06 g, 40 mmol), the chain transfer agent (4-cyano-4-[(phenethylsulfanylthiocarbonyl)sulfur Alkyl]valeric acid) (155 mg, 0.45 mmol) and initiator azobisisobutyronitrile (AIBN) (6.5 mg, 0.04 mmol) were dissolved in absolute ethanol (5 mL) and transferred to a locked tube. After the reaction mixture was subjected to three freeze-thaw cycles to remove air, the sealed tube was sealed under vacuum, and the sealed tube was immersed in an oil bath preheated to 75° C., and the polymerization reaction was carried out under magnetic stirring. After 12 hours, the reaction mixture was precipita...

Embodiment 3

[0051] The multifunctional modification method of embodiment 3 series polymers

[0052] The multifunctional polymer mainly uses amines containing different numbers of ethyleneimine repeating units to carry out ring-opening reaction on thiolactones containing hydrophobic functional groups, and the sulfhydryl groups generated in situ after ammonolysis undergo Michael addition reaction with PAOEMA. Taking the synthesis of P2B as an example, first, a DMF solution containing 2 equivalents (relative to the double bond in PAOEMA, the same below) of compound 2 (431mg, 1.68mmol) was added dropwise to 20 equivalents of diethylenetriamine (1.73g, 16.8 mmol) in DMF solution. After stirring the reaction at room temperature for 10 minutes, the 2 A DMF solution containing PAOEMA (150 mg, 0.01 mmol) was slowly added to the system under atmosphere. After continuing the reaction for 15 minutes, the reaction solution was precipitated and centrifuged three times in ether to finally obtain the l...

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Abstract

The invention relates to a polymer for conveying genes, a preparation method and application of the polymer, wherein the polymer is represented by a formula shown in the description.

Description

technical field [0001] The present invention is in the field of polymers for gene delivery. More specifically, the present invention relates to a polymer for gene delivery and its preparation method and application. Background technique [0002] Gene therapy has shown more and more significant advantages in the treatment of various refractory diseases. However, safe and efficient delivery of nucleic acids to target sites is still a great challenge in the application of gene therapy. In view of the safety issues of viral delivery vectors, non-viral gene delivery vectors have been greatly developed in recent years. Among them, polymer gene delivery carriers are more popular than others because of their advantages such as rapid preparation, easy modification, and chemical structure diversity. In order to achieve efficient gene transfection, delivery vectors must go through multiple obstacles inside and outside the cell, mainly including: (1) maintaining stability in blood; (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F120/20C08F8/34C08F8/14A61K48/00C12N15/87
Inventor 葛治伸查增仕
Owner UNIV OF SCI & TECH OF CHINA
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