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Aspergillus niger jxj A01 with activity of dissolving potassium, dissolving phosphorus and degrading organophosphorus pesticide and its application

A technology of JXJA01, organophosphorus pesticides, applied in the fields of application, organic fertilizers, potassium fertilizers, etc., to achieve the effect of reducing organophosphorus pesticide residues, improving and repairing farmland soil

Active Publication Date: 2020-09-08
JIUJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many microorganisms can dissolve potassium ore and turn insoluble ineffective potassium into soluble available potassium. At present, most of the researches are potassium-dissolving bacteria, and filamentous molds are rarely reported.

Method used

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  • Aspergillus niger jxj A01 with activity of dissolving potassium, dissolving phosphorus and degrading organophosphorus pesticide and its application
  • Aspergillus niger jxj A01 with activity of dissolving potassium, dissolving phosphorus and degrading organophosphorus pesticide and its application
  • Aspergillus niger jxj A01 with activity of dissolving potassium, dissolving phosphorus and degrading organophosphorus pesticide and its application

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Experimental program
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Embodiment Construction

[0025] 1 Screening of Aspergillus phosphorolyticus

[0026] Solid plate screening: The forest soil collected from Jiujiang City, Jiangxi Province was gradiently diluted with sterile water, and 0.2mL of the appropriate gradient dilution was applied to the phosphorus-dissolving solid culture of the industry standard NY / T 1847-2010 of the Ministry of Agriculture of China. Based on (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, MgSO 4 ·7H 2 O 0.3g, KCl 0.3g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, calcium phosphate or calcium phytate 10g, agar 18g, distilled water 1000mL, pH7.0), and after culturing in a 28°C incubator for 1-7d, observe the growth of bacteria and whether there is phosphorus dissolving around each colony Circle, select the strain JXJ A01 ( figure 1 ) for follow-up research.

[0027] 2 Identification of Aspergillus niger JXJ A01

[0028] According to the culture characteristics, morphological characteristics, 18S rRNA gene sequence and ITS sequence anal...

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Abstract

The present invention relates to the technical field of microbial application, in particular to a kind of Aspergillus niger JXJ A01 which has the activity of dissolving potassium, dissolving phosphorus and degrading organic phosphorus pesticides, and also relates to the ability of Aspergillus niger JXJ A01 to dissolve insoluble potassium, organic phosphorus and inorganic phosphorus, and degrade organic phosphorus. The application of phosphorus pesticides also involves the application of Aspergillus niger JXJ A01 in microbial fertilizers. The Aspergillus niger JXJ A01 is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with a preservation number of CGMCC No.13775. The Aspergillus niger JXJ A01 has a strong dissolving effect on insoluble phosphorus and potassium ore powder, and converts them into soluble phosphorus and potassium elements that can be absorbed by plants, and can degrade the organophosphorus pesticide omethoate to form non-toxic Soluble phosphate can reduce the concentration of pesticides and increase soil soluble phosphorus elements. The bacteria can be used to increase soil soluble available potassium and phosphorus, and repair the pollution of organic phosphorus pesticides to soil.

Description

technical field [0001] The present invention relates to the technical field of microbial application, in particular to the application of Aspergillus niger JXJ A01 capable of converting poorly soluble potassium, organic phosphorus and inorganic phosphorus into soluble potassium and phosphorus in microbial fertilizers, and to the application of Aspergillus niger JXJ A01 capable of degrading organic phosphorus pesticides. Application of Aspergillus JXJ A01 in reducing organic phosphorus pesticide residues in farmland, improving and repairing farmland soil. Background technique [0002] Phosphorus is an indispensable element for the normal growth and development of plants. More than 95% of the phosphorus in the soil is insoluble phosphorus that is difficult for plants to directly absorb and utilize. About 74% of the cultivated land in my country is deficient in phosphorus. The application of organic and chemical phosphorus fertilizers is the main source of soil phosphorus. How...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G1/00A62D3/02C09K17/14A62D101/26A62D101/45A62D101/40C09K101/00C12R1/685
CPCA62D3/02A62D2101/26A62D2101/40A62D2101/45C05B17/00C09K17/14C09K2101/00C05G5/45C12N1/145C12R2001/685C05D1/04C05D9/02C05F3/00C05F11/08C05G5/12C05F5/002C05F11/00
Inventor 张炳火李汉全
Owner JIUJIANG UNIV