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Non-invasive traceable biological material and preparation method thereof

A biomaterial and tracer technology, applied in the field of tissue engineering and medical materials, can solve the problems of self-tissue damage, immune rejection, insufficient donor source, etc., and achieve strong tissue penetration, high sensitivity, and enhanced strength. Effect

Inactive Publication Date: 2018-01-19
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, large-area defects usually need to be repaired by autologous or allogeneic tissue or organ transplantation, but autologous transplantation has the disadvantage of damaging its own tissue, while allogeneic transplantation has insufficient donor sources and immune rejection is its main defect

Method used

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  • Non-invasive traceable biological material and preparation method thereof
  • Non-invasive traceable biological material and preparation method thereof
  • Non-invasive traceable biological material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A method for preparing a non-invasive traceable biomaterial for repairing tissue damage, comprising the following steps:

[0053] 1. Buy Green, adjust the concentration of Green to 7mg / ml;

[0054] 2. After it is completely dissolved, put it into the mold, put the mold into liquid nitrogen to freeze, and then put it in a freeze dryer for 12 hours to freeze-dry to obtain the Green material, which is a three-dimensional collagen material, using 6kGy 60 Co electron beam irradiation is used to sterilize the green material;

[0055] 3. Place the Green material in a cross-linking solution containing 4mM EDC and 2.5mM NHS for cross-linking, place it at 37°C for 3-4 hours, and finally wash with PBS for 4-8 hours, and deionized water for 4-8 hours ;

[0056] 4. Complete CEST MRI monitoring with 11.7 T Bruker Avance spectrometer with 15mm coil. Place the sample in a direction parallel to the Z axis, the parameters are: slice thickness = 1mm, FOV = 1.4*1.3cm, matrix = 128x64, R...

Embodiment 2

[0059] The preparation of the non-invasive traceable material is the same as the operation steps in Example 1;

[0060] The concentration of Grignard in it is up to 10mg / ml, and the concentration of the crosslinking agent in the crosslinking solution is: 15mM EDC and 5mM NHS; image 3 and Figure 4 The experimental results are shown, where ** means P image 3 It can be seen from the images captured by CEST that the biological material has the highest MTR asymmetry values ​​at 1.8ppm; Figure 4 It can be seen that after the biomaterial is implanted in the body, the CEST contrast value gradually decreases as time increases, and the CEST contrast value decreases on the seventh day compared with the first day, and the difference is statistically significant; the decrease in CEST signal may be It is caused by changes in material composition and is closely related to the degradation of materials in vivo.

Embodiment 3

[0062] The preparation of the non-invasive traceable material is the same as the operation steps in Example 1;

[0063] The Grignin concentration is up to 15mg / ml, and the concentration of the crosslinking agent in the crosslinking solution is: 10mM EDC and 8mM NHS; Figure 5 and Figure 6 The experimental results are shown, and ** in the figure means P Figure 5 It can be seen from the images captured by CEST that the biological material has the highest MTR asymmetry values ​​at 1.8ppm; Figure 6 It can be seen that after the biomaterial is implanted in the body, the CEST contrast value gradually decreases as time increases, and the CEST contrast value decreases on the seventh day compared with the first day, and the difference is statistically significant; the decrease in CEST signal may be It is caused by changes in material composition and is closely related to the degradation of materials in vivo.

[0064] The experimental results show that the non-invasive traceable bio...

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Abstract

The invention belongs to the technical fields of tissue engineering and medical materials, relates to a non-invasive traceable biological material, and in particular relates to a non-invasive traceable biological material for tissue injury repair and a preparation method of the non-invasive traceable biological material. According to the technical scheme, freeze-dried Green solution is subjected to crosslinking by adopting a cross-linking agent, thus a material with a three-dimensional structure is prepared, and the tension and stability of the biological material are improved after crosslinking. According to the preparation method disclosed by the invention, the materials are easily available, the steps are simple, the safety of the obtained material is improved, the material can be moulded into different shapes according to demands, the non-invasive traceable biological material is used for repairing the tissue injuries and repairing the tissue injuries in the body and can be subjected to non-invasive tracing in vitro, and in addition, the non-invasive traceable biological material has good stability.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and medical materials, relates to a non-invasive traceable biological material, in particular to a non-invasive traceable biological material for repairing tissue damage and a preparation method thereof. Background technique [0002] Data show that tissue and organ defects and dysfunction caused by trauma, tumors and aging have become important factors that endanger human health, and the repair and functional reconstruction of defective tissues and organs is currently a difficult problem in this field. In the prior art, large-area defects usually need to be repaired by autologous or allogeneic tissue or organ transplantation, but autologous transplantation has the disadvantage of damaging its own tissue, while allogeneic transplantation has insufficient donor sources and immune rejection is its main defect . In recent years, the proposal, establishment and development of tissue enginee...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/50
Inventor 朱剑虹马富凯朱侗明王智富郑永涛
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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