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Method for Determining Fingerprint of Scrophulariaceae Extract

A fingerprint and extract technology, applied in the field of compound identification, can solve the problems of few characteristic peaks, weak specificity, long time, etc., and achieve the effect of objective conclusion, standardized production operation, and convenient operation.

Active Publication Date: 2020-09-04
YANGTZE RIVER PHARM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to objectively and effectively evaluate or control the quality of Scrophulariae Radix Scrophulariae by only measuring the content of certain components.
Chinese invention patent ZL200810184570.5 discloses a Scrophulariaceae formula granule and its preparation method and quality control method. It applies infrared spectroscopy technology to the quality control of traditional Chinese medicine, and quickly solves a primary problem in the macroscopic quality control of traditional Chinese medicine - traditional Chinese medicine The identification of authenticity, but the infrared fingerprints used in this method for quality identification, its specificity is not strong
Chinese invention patent application 201610423663.3 discloses a method for establishing the fingerprint of the medicinal preparation of Scrophulariaceae Scrophulariae. The fingerprint is established by high performance liquid chromatography, and three chromatographic peaks of compounds such as harpagoside, harpagoside and cinnamic acid are obtained, but less characteristic peaks
In addition, the use of a general-purpose reversed-phase C-18 column makes the analysis time longer

Method used

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  • Method for Determining Fingerprint of Scrophulariaceae Extract
  • Method for Determining Fingerprint of Scrophulariaceae Extract
  • Method for Determining Fingerprint of Scrophulariaceae Extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Collection of Radix Scrophulariae Medicinal Materials

[0029] Scrophulariaceae collection information is shown in Table 1

[0030] Table 1

[0031]

Embodiment 2

[0032] Example 2 Obtaining the Fingerprint of Scrophulariaceae Scrophulariae Extract

[0033] (1) Experimental instruments, reagents and samples

[0034] Reference substances: harpagoside, harpagoside, aucubin, verbascoside, angroside C and cinnamic acid (China Institute for the Control of Pharmaceutical and Biological Products).

[0035] Instrument: Agilent 1260 high performance liquid chromatograph

[0036] (2) Chromatographic conditions and method validation

[0037] ①Wavelength selection

[0038] The main active ingredient of Scrophulariaceae is iridoids. Due to the lack of conjugated double bond structure in the molecular core structure, most iridoids, such as harpaside, have no absorption at wavelengths above 250nm , so the fingerprint spectrum of Scrophulariaceae extract measured at the wavelength of 280nm~290nm will lose a lot of peak information, so the detection wavelength is set at 210nm.

[0039] ②Mobile phase selection

[0040] Most of the mobile phase system...

Embodiment 3

[0055] Embodiment 3 method verification

[0056] Take the extract for the test solution, inject 6 times continuously, and investigate the consistency of the relative retention time and relative peak area ratio of the chromatographic peaks, so as to investigate the precision of the instrument. The test results are shown in Tables 1-3. The results showed that the RSD values ​​of the relative retention time ratios of each common peak were within 1%, the relative peak area ratio RSD values ​​were within 3%, and the similarity was greater than 0.99, which indicated that the precision of the instrument was good.

[0057] Table 1 Precision test results of methodological investigation of fingerprints of Scrophularia scrophulariae extract (relative retention time)

[0058]

[0059] Table 2 Precision test results of methodological investigation of fingerprints of Scrophularia scrophulariae extract (relative peak area)

[0060]

[0061] Table 3 Precision test results (similarity) ...

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Abstract

The invention relates to a method for measuring radix scrophulariae extract fingerprint spectrums. The method is characterized in that water is used as an extracting solution to prepare the radix scrophulariae extract; the radix scrophulariae extract is used as a detection object; the fingerprint spectrum methods of the radix scrophulariae medicinal material, herbal medicines, extracts and preparations are established, the more comprehensive spectrum information is obtained, ten common peaks of the radix scrophulariae extract are verified, and the six peaks are indicated; the quality can be comprehensively and quickly detected by combining with the information of multiple spectrum peaks in the fingerprint spectrums, and the comprehensive quality detection and quality control can be favorably performed. The method has the advantages that the positive function on effective feeding guidance on crude drugs in clinical medicine application and production process and ensuring of reliable quality is realized; the operation is simple and rapid, the quality of the radix scrophulariae extract is evaluated, and the conclusion is more objective and accurate.

Description

technical field [0001] The invention relates to a method for identifying compounds, in particular to a method for obtaining fingerprints extracted from Scrophulariae scrophulariae extract, so as to realize the quality monitoring of drugs developed by Scrophulariae scrophulariae and make the detection process more stable. Background technique [0002] Scrophulariaceae is the dried root of Scrophularia ningpoensis Hemsl. Nature is sweet, bitter, salty, slightly cold. Return lung, stomach, kidney channel. Function Clearing heat and cooling blood, nourishing yin and reducing fire, detoxifying and dispelling stagnation. It is used for heat entering the camp and blood, warm toxins causing spotting, febrile disease affecting yin, red tongue and polydipsia, fluid injury and constipation, bone steaming and labor cough, red eyes, sore throat, diphtheria, scrofula, carbuncle and sore. It was first recorded in "Shen Nong's Materia Medica" and was listed as a middle grade. Scrophular...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 王红敏盛卫国范明松刘承伟何爱琴
Owner YANGTZE RIVER PHARM GRP CO LTD
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