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Method for detecting MFG-E8

A MFG-E8, detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited ELISA detection ability, achieve the effect of reducing hook effect, low detection limit and good precision

Inactive Publication Date: 2018-01-30
武汉市中西医结合医院
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Problems solved by technology

At present, the enzyme-linked immunosorbent assay (ELISA) is mainly used to detect MFG-E8, but the detection ability of ELISA is limited, and there are shortcomings such as insufficient sensitivity.

Method used

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  • Method for detecting MFG-E8
  • Method for detecting MFG-E8
  • Method for detecting MFG-E8

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Experimental program
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Embodiment Construction

[0020] By providing a detection method for MFG-E8, the present invention solves the shortcomings of limited detection ability and insufficient sensitivity when detecting MFG-E8 in the existing ELISA method.

[0021] In order to solve the above defects, the main ideas of the embodiments of the present invention are:

[0022] The detection method of the embodiment of the present invention MFG-E8 comprises:

[0023] 1) Preparation of testing samples and standards;

[0024] 2) Coating the anti-human MFG-E8 protein mouse monoclonal antibody;

[0025] 3) The detection sample and the standard were contacted with the coated anti-human MFG-E8 protein mouse monoclonal antibody, and anti-human MFG-E8 protein sheep polyclonal antibody, horseradish peroxidase-labeled The rabbit anti-sheep IgG antibody and the luminescent substrate were contacted, and after the dark reaction, the chemiluminescence value was measured and the MFG-E8 content in the sample was calculated.

[0026] In order t...

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Abstract

The invention provides a method for detecting MFG-E8. The method includes 1), preparing detection samples and standard substances; 2), coating and adsorbing anti-human MFG-E8 protein mouse monoclonalantibodies in coated plates; 3), allowing the detection samples and the standard substances to be in contact with the coated anti-human MFG-E8 protein mouse monoclonal antibodies, adding anti-human MFG-E8 protein sheep polyclonal antibodies, anti-sheep IgG rabbit antibodies labeled by horseradish peroxidase and luminescence substrates into the detection samples and the standard samples, allowing the detection samples and the standard substances to be in contact with the anti-human MFG-E8 protein sheep polyclonal antibodies, the anti-sheep IgG rabbit antibodies labeled by the horseradish peroxidase and the luminescence substrates, carrying out light protective reaction, measuring chemiluminescence values and computing the content of the MFG-E8 in the detection samples. The method has the advantages that the method is good in precision, low in detection limit, high in sensitivity and wide in linear range, clinical application requirements can be met, and the like.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and in particular relates to a detection method of MFG-E8. Background technique [0002] Milk fat globule epidermal growth factor Ⅷ (milk fat globule epidermal growth factor 8, MFG-E8) was initially identified as a product of the lactating mammary gland, secreted by activated macrophages and immature dendritic cells. MFG-E8 acts as a bridging molecule that binds to phosphatidylserine on the surface of apoptotic cells, enhances the phagocytosis of apoptotic cells by macrophages and dendritic cells, regulates the occurrence of inflammatory responses, and participates in autoimmune diseases , inflammatory diseases, tumors, etc. Studies have shown that MFG-E8 can alleviate tissue damage in systemic lupus erythematosus, and also play an important role in the clearance of apoptosis in chronic obstructive pulmonary disease. At present, enzyme-linked immunosorbent assay (ELISA) is mainly used to de...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/535
Inventor 崔天盆熊银李妹
Owner 武汉市中西医结合医院
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