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Dendrimer-modified gold nanoparticles and its preparation method and application

A technology of gold nanoparticles and dendrites, which is applied in the field of gold nanoparticles modified by dendrimers and its preparation, to achieve the effects of high reaction efficiency, single molecular weight distribution, and reduced biological toxicity

Active Publication Date: 2020-07-03
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, it has not been found that azide chitosan can be coupled with alkyne-containing dendritic polyamidoamines into a biocompatible and degradable polyamide-amine using click chemistry without adding other reducing agents. Chitosan-based polyamide-amine dendrimers acted as stabilizers and reducing agents to synthesize gold nanoparticles in one step, resulting in stable, biocompatible and degradable gold nanocomposites and their applications have not yet been reported

Method used

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  • Dendrimer-modified gold nanoparticles and its preparation method and application
  • Dendrimer-modified gold nanoparticles and its preparation method and application
  • Dendrimer-modified gold nanoparticles and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0062] (1) Azide modified chitosan (CS-N 3 )Synthesis

[0063] Azidoacetic acid (N 3 -CH2-COOH) was dissolved in N-N-dimethylformamide (DMF), and then sequentially added 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) was activated for 30 minutes to obtain azidoacetic acid mixed solution; after activation, chitosan (CS molecular weight was 2000, deacetylation degree 85%) was dissolved in pure water, and then slowly added to the above In the mixed solution of azidoacetic acid, react at room temperature at 35°C for 12h. The product was dialyzed in pure water for 2 days, freeze-dried to obtain the product azide chitosan; wherein, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride The molar ratio of salt (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid is 1:1.5:1.5:2; Add 5g of azide; said pure water is calculated by adding 5g of chitosan per 10ml.

[0064] (2) Synthesis of alkyne-containing p...

Embodiment 2

[0067] (1) Azide modified chitosan (CS-N 3 )Synthesis

[0068] Azidoacetic acid (N 3 -CH2-COOH) was dissolved in N-N-dimethylformamide (DMF), and then sequentially added 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) was activated for 4 hours to obtain azidoacetic acid mixed solution; after activation, chitosan (CS molecular weight was 20000, deacetylation degree 40%) was dissolved in pure water, and then slowly added to the above Azidoacetic acid mixed solution, reacted at room temperature for 24h. The product was dialyzed in pure water for 3 days, and freeze-dried to obtain the product azide chitosan. Among them, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid The molar ratio is 1:10:10:10; the N,N-dimethylformamide is based on the addition of 1g azide per 10ml; the pure water is based on the addition of 0.5g chitosan per 10ml sugar me...

Embodiment 3

[0072] (1) Azide modified chitosan (CS-N 3 )Synthesis

[0073] Azidoacetic acid (N 3 -CH2-COOH) was dissolved in N-N-dimethylformamide (DMF), and then sequentially added 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) was activated for 2 hours to obtain azidoacetic acid mixed solution; after activation, chitosan (CS molecular weight was 10000, deacetylation degree 60%) was dissolved in pure water, and then slowly added to the above Azidoacetic acid mixed solution, reacted at room temperature for 16h. The product was dialyzed in pure water for 2 days, and freeze-dried to obtain the product azide chitosan. Among them, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid The molar ratio is 1:5:5:5; the N,N-dimethylformamide is based on the addition of 3g azide per 10ml; the pure water is based on the addition of 3g chitosan per 10ml count.

[00...

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Abstract

The invention discloses dendritic macromolecule modified gold nano particles as well as a preparation method and application thereof. The method comprises the following steps: performing azide modification on chitosan, and synthesizing glycan coupled dendritic polyamide-amine (CS-PAMAM) with alkynyl-containing dendritic polyamide-amine through a click reaction; and synthesizing gold nano-particleswith uniform grain size in one stop by using CS-PAMAM as a stabilizer and a reducer in one stop under the condition of not adding other reducer to obtain dendritic macromolecule modified gold nano particles having relatively small grain size. The gold nano particles have high stability and good biocompatibility besides favoring reduction of product cytotoxicity, massive amine functional groups are contained on the surface, target property is given to the gold nano particles, and an important application prospect is showed in gene transfer and tumor diagnosis integration. The dendritic macromolecule modified gold nano particles have excellent transfection, can be used as a medicine controlled-release vector, and has a potential application value in the field of medicine co-transfer.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to a dendrimer-modified gold nanoparticle and a preparation method and application thereof. Background technique [0002] Gene therapy is currently an effective method for the treatment of cancer and innate immune system diseases. The key to the implementation of this technology is to select the appropriate gene carrier and gene introduction method, so that the gene can be expressed in cells safely, efficiently, controllably and stably. Viral vectors are limited in clinical application due to their high risk, immune response, and incapability of large-scale production. Therefore, liposomes and other cationic polymers with high transfection efficiency are increasingly valued. [0003] Gold nanoparticles are the earliest studied category of metal nanoparticles, which refer to gold nanoparticles with sizes ranging from 1nm to 100nm. Gold nanoparticles have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/61A61K48/00A61K41/00A61K49/00A61P35/00
CPCA61K41/0052A61K48/0041A61K48/0091A61K49/00
Inventor 俞思明李国巍马栋薛巍
Owner JINAN UNIVERSITY
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