Nutrient substrate for pheretima aspergillum as well as preparation method and culture method of pheretima aspergillum
A technology of worms and nutrient substrates, which is applied in the field of breeding of worms, can solve the problems of unguaranteed quality of earthworm medicinal materials, difficulties in artificial breeding, and shortage of wild earthworms, so as to improve the rural ecological environment and fecundity High, the effect of protecting the ecological environment
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Embodiment 1
[0030] Embodiment one: the preparation of the nutrient matrix of C. chinensis
[0031] 1. Temperature control during fermentation
[0032]Use EM strains to treat plant extract residues (mix EM bacteria and plant extract residues at a mass ratio of 1:10000, pile them into a strip pile with a width and height of about 1m, and control the humidity at 60% to 80 % for fermentation), the plant extract residue used in the present embodiment is the mixture of Ginkgo biloba, Radix Astragali, Codonopsis pilosula, Polygonatum ulmoides, Eucommia ulmoides, chrysanthemum and Prunella vulgaris after the active ingredients are extracted by water, and the temperature on the first day of fermentation can be When it reaches 50°C, it quickly enters the high temperature period and promotes the fermentation reaction. On the 18th day, the temperature of the material can reach 68°C, and the high temperature period lasts for 5 to 7 days, and then the temperature is slowly lowered to make the material...
Embodiment 2
[0040] Embodiment two: the nutrient substrate prepared in embodiment one is mixed with sludge as culture substrate
[0041] Domestic sludge refers to the sludge produced after the sewage treatment plant treats urban domestic sewage. In this example, the sludge produced by the sewage treatment plant in Yangling Agricultural High-tech Industry Demonstration Zone, Shaanxi Province was used.
[0042] The influence of culture medium is shown in Table 1. The quality and quantity of each group showed different degrees of growth under different proportions. Among them, the growth, development and reproduction were the best when the mass ratio of fermentation extraction residue to domestic sludge was 7:3, the penetration time was the fastest, the feed intake was the largest, the adaptability was the strongest, and the daily weight gain and daily weight ratio were the best. The multiplication factor was the largest; the ratio of 6:4 as substrate was the second; the ratio of 8:2 was th...
Embodiment 3
[0045] Embodiment three: the influence of humidity on the worm
[0046] The substrate used in this example is the substrate with the optimal ratio in Example 2, that is, the substrate with a mass ratio of fermentation extraction residue to domestic sludge of 7:3.
[0047] Substrate humidity has a great influence on the growth, development and reproduction of C. Table 2 shows the effect of different substrate humidity on the culture of worms. When the humidity is 45%, the breeding effect is the best, there is no death or escape, and the feed intake is large, the adaptability is strong, the growth and reproduction are in good condition, the range of activities is normal, and the daily weight gain and daily multiplication multiples reach the maximum. When the humidity is 40%, the effect is second. When the humidity is lower than 30% and higher than 55%, the worm escapes and dies, the adaptability becomes poor, and the daily multiplication factor decreases.
[0048] Table 2 Comp...
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