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Composition gel and applications

A composition and gel technology, applied in the fields of regenerative medicine and biomedicine, can solve the problems of stem cell aging, failure, filling failure, etc., and achieve the effects of long-lasting degradation cycle, wide source and good stability

Inactive Publication Date: 2018-02-16
李媚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN200610152104.X discloses a human soft tissue filler for injection composed of cell components and hyaluronic acid and its preparation method, but the filler contains very little 2.0-30 mg / ml, and it is easy to become transparent in a short time Hyaluronic acid is degraded, and at the initial stage of injection, there is a risk of filling failure due to too little collagen secreted by cells
At present, stem cell transplantation is the direct transplantation of stem cells. Often, after obtaining sufficient quantity and shape of stem cells in vitro, they must be implanted into the hands of patients within a day or even several hours, otherwise the stem cells will over-differentiate and age. Big constraints, unable to industrialize

Method used

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  • Composition gel and applications
  • Composition gel and applications
  • Composition gel and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Preparation of Umbilical Cord Mesenchymal Stem Cells

[0025] Wash the fresh umbilical cord with normal saline to clean the blood in the blood vessels in the umbilical cord;

[0026] Cut the umbilical cord tissue into minced meat and transfer it to a 50ml centrifuge tube, 30ml per tube;

[0027] Add 0.25% I collagenase to each tube, mix repeatedly, and digest in a constant temperature shaker at 37°C for 20 minutes;

[0028] Add the same volume of 0.25% trypsin to each tube and continue to digest for 20 minutes until no large tissue pieces are visible to the naked eye;

[0029] Add 30ml α-MEM containing FBS to each tube to stop the digestion, remove undigested tissue, add 3 times the PBS medium, centrifuge at 3000rpm / min for 3min, discard the supernatant, and collect the cells;

[0030] Add an equal volume of FBS-containing α-MEM to resuspend the cells, count the cells, inoculate 3×105 cells into a culture flask after counting, and culture in a 5% CO2 incuba...

Embodiment 2

[0033] Embodiment 2, preparation of adipose stem cells

[0034] Under sterile conditions, divide the adipose tissue into 50ml centrifuge tubes, 30ml in each tube;

[0035] Add an equal volume of 0.2% type Ⅰ collagenase to each tube, mix thoroughly, and transfer to a constant temperature oscillator at 37°C for digestion for 30 minutes;

[0036] Add 30ml of α-MEM containing FBS to each tube to stop digestion, add 2 times the volume of PBS medium, centrifuge at 1500rpm / min for 5min, discard the supernatant, add PBS to wash repeatedly, and resuspend the cells in DMEM medium;

[0037] Inoculate into 25mm petri dish, place in 37 ℃, the volume fraction is 5% CO2 incubator culture;

[0038] After 48 hours, replace half of the medium for the first time, subculture when the cells grow to 75%-90% confluence, and replace the culture medium every 3 days;

[0039] Discard the medium, add 5ml of PBS solution, shake gently and discard the PBS solution, repeat once; add 2mL of 0.2% trypsin t...

Embodiment 3

[0044] Embodiment three, composition gel properties

[0045] In this experiment, the gel properties of different compositions of the present invention were compared, and the solvent of the biodegradable material gel was physiological saline, and the specific data are shown in Table 1.

[0046] Table 1.

[0047]

[0048]

[0049] It can be seen from the experimental data that the greater the concentration of the biodegradable material in the composition gel of the present invention, the more stem cells are contained in the composition gel.

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Abstract

The invention relates to composition gel and applications, belonging to the field of regenerative medicine and biological medicine. The composition gel is mainly composed of biodegradable material geland stem cells. The composition gel provided by the invention has the advantages that the degradation period is permanent, the stability is good, and the source of stem cell raw materials is wide.

Description

technical field [0001] The invention relates to a composition gel and its application, in particular to a composition gel containing stem cells and a biodegradable material gel and its application, belonging to the fields of regenerative medicine and biomedicine. Background technique [0002] Mesenchymal stem cells (MSC, mesenchymal stem cells) are derived from a class of pluripotent stem cells in the mesoderm and ectoderm in the early stages of development. And the promotion of stem cell implantation, immune regulation and self-replication and other characteristics have attracted increasing attention. Mesenchymal stem cells are widely distributed in bone marrow, periosteum, cancellous bone, fat, synovium, skeletal muscle, fetal liver, deciduous teeth, umbilical cord, and umbilical cord blood. , cartilage, muscle, tendon, ligament, nerve, liver, myocardium, endothelium and other tissue cells still have multi-directional differentiation potential after continuous subculture ...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/58A61L27/54A61K35/28A61K9/06A61K47/38A61K47/36A61K47/34A61K47/42A61P39/06A61P19/02A61P17/02A61P35/00A61P35/02A61P1/16A61P13/12A61P25/00A61P3/10A61P25/16A61P25/28
CPCA61K9/06A61K35/28A61K47/34A61K47/36A61K47/38A61K47/42A61L27/3834A61L27/3852A61L27/54A61L27/58A61L2300/412A61L2430/00A61L2430/06
Inventor 李媚
Owner 李媚
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