Preparation process of high-yield collagen fibers

A collagen fiber and preparation technology, which is applied in the field of high-yield collagen fiber preparation technology, can solve the problems of large-scale production, large-scale production, mixing of impurities, long extraction process cycle, etc., and achieve long bursting strength , saving production site, high tensile strength effect

Active Publication Date: 2022-02-11
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] In order to solve the problems of long extraction process cycle, large-scale production, and possible mixing of foreign proteins in the prior art, the present invention provides a high-yield collagen fiber preparation process, which does not destroy collagen during the preparation process. structure, after a series of conditional treatments, collagen fibers are finally obtained, and collagen sponges prepared with collagen fibers have higher tensile strength, bursting strength and longer degradation cycle

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  • Preparation process of high-yield collagen fibers

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Effect test

Embodiment 1

[0069] The present embodiment provides a kind of preparation technology of high-yield collagen fiber, and described preparation technology comprises the following steps:

[0070] (1) Cutting: Select pigskin and cut it into thin slices to form tissue slices;

[0071] (2) Pre-treatment: Soak the tissue piece obtained in step (1) in a sodium chloride solution with a concentration of 1.5%, mix it for the first time with magnetic stirring, then wash with water to remove the sodium chloride solution, and pour it into the soaked tissue Add ethanol solution to the mixture, the concentration of the ethanol solution is 90wt%, magnetic stirring is carried out for the second mixing, and then the ethanol solution is removed by washing with water to form a pre-treated tissue;

[0072] (3) Enzyme reaction: Add 15wt% pretreated tissue to a weakly alkaline solution with a pH of 7.6, then add 10wt% trypsin from the pretreated tissue, react for 10 hours at 25°C, and add 1M The hydrochloric acid...

Embodiment 2

[0077] This embodiment provides a collagen fiber with high yield, and the preparation process includes the following steps:

[0078](1) Cutting: Select cowhide, cut into thin slices with length and width of 0.3-1.5mm to form tissue blocks;

[0079] (2) Pretreatment: Soak the tissue piece obtained in step (1) in a sodium chloride solution with a concentration of 1.5%, and mix for the first time at a ratio of 10ml of sodium chloride solution per gram of tissue, Stir magnetically at 25-37°C for 36 hours, then wash to remove the sodium chloride solution; add 5ml of ethanol solution with a concentration of 60wt% per gram of soaked tissue for the second mixing, and stir magnetically at 25-37°C After 12 hours, wash with water to remove the ethanol solution to form a pre-treated tissue;

[0080] (3) Enzyme reaction: Add 15wt% pre-treated tissue to an acidic solution with a pH of 2.5, then add 10wt% pepsin in the pre-treated tissue, react for 10 hours at 28°C, and add 1M hydroxide aft...

Embodiment 3

[0085] The present embodiment provides a kind of preparation technology of high-yield collagen fiber, and described preparation technology comprises the following steps:

[0086] (1) Cutting: Select bovine Achilles tendon and cut it into small cubic pieces of 0.3-1.0mm to form tissue blocks;

[0087] (2) Pretreatment: Soak the tissue piece or tissue block obtained in step (1) in 1.0% sodium chloride solution, and perform the first mixing at the ratio of adding 10ml of sodium chloride solution per gram of tissue, Stir magnetically at 25-37°C for 24 hours, then wash with water to remove the sodium chloride solution, add 10ml of ethanol solution with a concentration of 75wt% to each gram of soaked tissue for the second mixing, stir magnetically at 25-37°C for 24 hours, wash with water Remove the ethanol solution to form a pre-treated tissue;

[0088] (3) Enzyme reaction: In the acetic acid-sodium acetate buffer solution with a pH of 6.0, add 15wt% of the pretreated tissue obtain...

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Abstract

The invention relates to a preparation process of high-yield collagen fibers. The method comprises comprises the following steps: selecting animal tissues rich in collagen, and cutting into slices or blocks to form tissue slices or tissue blocks; carrying out pretreatment, enzyme reaction, crushing and alkali treatment to obtain collagen with a triple-helical structure; and preparing the collagen sponge through a freeze-drying process. The method disclosed by the invention has the beneficial effects that on the premise of removing terminal peptide and elastin and inactivating viruses, the method has higher extraction efficiency, impure protein is thoroughly removed, and the prepared collagen is relatively high in purity. Compared with acid-soluble collagen sponge, the collagen sponge prepared by the invention has higher tensile strength and bursting strength and longer degradation period. Meanwhile, the use amount of process water is small, the scale of a reaction device is smaller than that of other acid or enzyme extraction methods, and the production site can be greatly saved.

Description

technical field [0001] The invention relates to a collagen fiber, in particular to a preparation process for a high-yield collagen fiber used in medical devices. Background technique [0002] Collagen is one of the main components of the extracellular matrix, accounting for about 1 / 3 of the total protein mass of mammals. It is the main component of connective tissues or organs such as skin, ligaments, cartilage, and tendons. Collagen has unique physical and chemical properties and excellent Biocompatibility, degradability, low immunity and other advantages make it easy to be absorbed in the body, strong hydrophilicity, non-toxic and safe, and become one of the choices of biomedical materials. Generally, it is rich in collagen fibers Extraction of animal tissues such as skin, tendons, bones, etc. Collagen extraction methods usually include alkali method, salt method, acid method, and enzymatic method. According to its solubility, collagen can be divided into: collagen fibers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F4/00D01F1/10C08J9/28C08L89/00C08L5/08C07K14/78C07K1/14C12P21/06
CPCD01F4/00D01F1/10C08J9/28C07K14/78C12P21/06C08J2201/0482C08J2389/00C08J2405/08
Inventor 张娈张伟张伊骁孙冰冰
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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