Silver nanocluster fluorescent probe based on polyethyleneimine protection and application thereof in detecting metronidazole

A polyethylenimine and fluorescent probe technology, applied in the field of fluorescent probes, can solve problems such as teratogenicity, harm to human and other animal health, and genotoxicity

Inactive Publication Date: 2018-02-16
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the cumulative dose of metronidazole exceeds a certain value, it has potential teratogenic, carcinogenic, mutagenic and genotoxic effects, endangering the health of humans and other animals

Method used

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  • Silver nanocluster fluorescent probe based on polyethyleneimine protection and application thereof in detecting metronidazole
  • Silver nanocluster fluorescent probe based on polyethyleneimine protection and application thereof in detecting metronidazole
  • Silver nanocluster fluorescent probe based on polyethyleneimine protection and application thereof in detecting metronidazole

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Firstly, 3.75 μM PEI was dissolved in 15 ml deionized water, stirred for 30-60 min, and 3.75 μM AgNO was added to the above solution. 3 After stirring for 2 h, add HCl with a concentration of 1 M to adjust the pH value to 5, add 37.5 μM AA, and put it into a microwave synthesizer for reaction. , 60, and 120 min were used to measure the fluorescence intensity, and polyethyleneimine-protected silver metal nanoclusters AgNCs were obtained at different reaction times.

[0029] The results showed that the fluorescence intensity of AgNCs obtained was the highest when the reaction time was 15 min.

Embodiment 2

[0031] Dissolve 3.75 μM PEI in 15 ml deionized water, stir for 30-60 min; then add 3.75 μM AgNO to the above solution 3 , after continuing to stir for 2 h, add HCl with a concentration of 1 M to adjust the pH value to 5, then divide the solution into 4 parts, put in different proportions of AA, Ag + The ratios to AA are 1:1, 1:5, 1:10, 1:15 respectively, reacted in a microwave synthesizer, reacted for 15 min on the basis of Example 1, and filtered to obtain different Ag + Polyethyleneimine-protected silver metal nanoclusters AgNCs under ratio conditions with AA.

[0032] The results show that: when in solution, the added Ag + The ratio to AA is 1:10, which is AgNO 3 The fluorescence intensity of AgNCs was the highest when AA was 3.75 μM and AA was 37.5 μM.

Embodiment 3

[0034] Dissolve 3.75 μM PEI in 15 ml deionized water, stir for 30-60 min; then add 3.75 μM AgNO to the above solution 3 After continuing to stir for 2 h, the solution was divided into 5 parts, and the pH value was adjusted. Add HCl and NaOH with a concentration of 1 M to the solution respectively, adjust the pH value of the solution to 3, 5, 7, 9, 11, add 37.5 μM AA to it on the basis of Example 1 and 2, and put it in the microwave The reaction was carried out in a synthesizer for 15 min, and after filtration, polyethyleneimine-protected silver metal nanoclusters AgNCs under different pH conditions were obtained.

[0035] The results showed that the fluorescence intensity of the obtained AgNCs was the highest when the pH value of the reaction was 5.

[0036] The results showed that the AgNCs with the highest fluorescence intensity were prepared based on the above experimental conditions: AgNO 3 The raw material ratio (molar ratio) to AA is 1:10, the pH value is set to 5, and...

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Abstract

A silver nanocluster fluorescent probe based on polyethyleneimine protection and application thereof in detecting metronidazole belong to the technical field of fluorescent probes. Silver nanoclustershave great Stokes shift (about 90 nm), and can exist in a solution with extreme acidic pH and high ion concentration and release stable fluorescence when exposed to ultraviolet for long time; all these characteristics are good for the extensive application of the nanoclusters in medicine and biological fields. The silver nanocluster probe in Tris-HCl buffer solution is used to detect metronidazole; the results show that the linear range is 0-10 mu M, and a detection limit is 0.01 mu M. Meanwhile, the silver nanocluster probe has successfully carried out entity detection on metronidazole in urea and achieved good results, it is indicated that the silver nanocluster probe is capable of detecting metronidazole residue in body fluid of a human body.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and in particular relates to a polyethyleneimine-based silver metal nano-cluster fluorescent probe and its application in detecting metronidazole. Background technique [0002] Metronidazole is mainly used for clinical treatment and prevention of infections caused by anaerobic bacteria, such as respiratory and digestive tract infections. After years of practice and medical proof, metronidazole is a drug with high efficiency, quick action and little harm to the human body, which can minimize the growth of bacteria and eliminate infection. It is widely used to treat diseases caused by protozoa or anaerobic bacteria, and it also has the effect of promoting the growth of cattle, pigs and poultry and improving feed efficiency. However, when the cumulative dose of metronidazole exceeds a certain value, it has potential teratogenic, carcinogenic, mutagenic and genotoxic effects, endangering ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/58G01N21/64
CPCC09K11/025C09K11/58G01N21/6428G01N2021/6432
Inventor 孟磊徐娜刘荣霞
Owner JILIN INST OF CHEM TECH
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