Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of blood cell small peptide rare-earth lanthanum chelate

A rare earth lanthanum and chelate technology, which is applied in the field of preparation of organic trace elements, can solve the problems that the preparation method of the rare earth lanthanum chelate of blood cell small peptides has not yet seen research reports and other problems, and achieves low cost, high production efficiency and technological process. simple effect

Inactive Publication Date: 2018-02-16
陈石良
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few researches and developments on rare earth protein peptide chelates in China at present, and no research reports have been found on the preparation method of rare earth lanthanum chelates for blood cell small peptides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of blood cell small peptide rare-earth lanthanum chelate
  • Preparation method of blood cell small peptide rare-earth lanthanum chelate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of pig blood cells: Collect 1000L of fresh pig blood that has been anticoagulated, and use a tube centrifuge to continuously centrifuge to obtain 420L of blood cells and 580L of plasma. ; Add 0.5% food-grade sodium citrate of fresh pig blood weight during anticoagulant treatment;

[0026] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 420L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min to prepare 840L of hemolysis;

[0027] (3) Enzymolysis: Heating the hemolysis solution to 52°C while continuing to stir, adjusting the pH value to 8.0 with NaOH solution, then adding 0.5% protease by weight of the blood cell solution, stirring constantly, and detecting the enzymolysis solution every one hour pH value, when the pH value drops below 8.0, adjust the pH value to 8.0 with alkali supplementation solution, heat the enzymolysis solution for 10 hours, heat the enzymolysis sol...

Embodiment 2

[0035] (1) Preparation of pig blood cells: collect 1000L of fresh pig blood that has been anticoagulated, and use a tubular centrifuge to continuously centrifuge to obtain 438L of blood cells and 562L of plasma. Collect the blood cells, and spray dry the plasma to make plasma protein powder ; Add 0.5% food-grade sodium citrate of fresh pig blood weight during anticoagulant treatment;

[0036] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 440L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min, and 878L of hemolysis is obtained;

[0037] (3) Enzymolysis: Heating the hemolyzate to 50°C with continuous stirring, adjusting the pH value to 7.5 with NaOH solution, then adding 0.8% protease by weight of the blood cell solution, stirring constantly, and detecting the concentration of the enzymatic solution every one hour PH value, when the pH value drops below 7.5, adjust the pH value to 7.5 with a...

Embodiment 3

[0044] (1) Preparation of porcine blood cells: collect 1000L of fresh porcine blood after anticoagulant treatment, and use a tube centrifuge to continuously centrifuge to obtain

[0045] 410L of blood cell fluid and 590L of plasma fluid were collected, and the plasma fluid was spray-dried to make plasma protein powder; 0.5% food-grade sodium citrate of fresh pig blood was added to the anticoagulant treatment;

[0046] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 410L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min to prepare 820L of hemolysis;

[0047](3) Enzymolysis: Heating the hemolyzate to 55°C with continuous stirring, adjusting the pH value to 7.8 with NaOH solution, then adding 1.0% protease by weight of the blood cell solution, stirring constantly, and detecting the enzymatic hydrolyzate at intervals of one hour pH value, when the pH value drops below 7.8, adjust the pH value to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of blood cell small peptide rare-earth lanthanum chelate. The preparation method mainly includes: preparing pig blood cell liquid, performing hemolysis, performing enzymolysis, centrifuging, performing ultrafiltration, performing nanofiltration, chelating and performing spraying drying to obtain the blood cell small peptide rare-earth lanthanum chelate. The preparation method has the advantages that pig blood which is cheap and wide in source is used as the protein peptide source, rare-earth lanthanum salt is used as the lanthanum source, lanthanumis chelated with small peptides, and the preparation method is simple in process flow, low in cost and suitable for large-scale industrial production; the prepared blood cell small peptide rare-earthlanthanum chelate which is a novel effective feed additive is high in small-molecule peptide content and high in chelating rate.

Description

technical field [0001] The invention relates to a preparation method of organic trace elements, in particular to a preparation method of rare earth lanthanum chelate of small blood cell peptide. Background technique [0002] Small peptide trace element chelate is the third-generation new trace element feed additive that has developed rapidly at home and abroad in recent years. It is formed by the coordination reaction of trace element metal ions necessary for animal growth and small molecular peptides or short peptide substances. Compounds with ring structures. Since the small peptide trace element chelate is close to the natural form of trace elements in animals, it has good chemical stability, high biological potency, easy digestion and absorption, anti-interference, non-irritating, and non-toxic effects. Supplementing trace elements while meeting the nutritional needs of protein plays a very important role in the growth of animals, especially the healthy growth of young ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 陈石良
Owner 陈石良