HPLC method for measuring contents of 6 endogenous hormones in salvia miltiorrhiza bunge at the same time

A technology of endogenous hormones and content, applied in the field of chemical analysis, can solve problems such as flowering failure, pests and diseases, affecting the development of Sichuan salvia miltiorrhiza, and achieve the effect of high sample recovery and excellent linear relationship

Active Publication Date: 2018-02-16
SICHUAN AGRI UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional propagation method of Salvia miltiorrhiza is mainly by seeds and split roots. The main production areas of Danshen in Shandong, Henan, Shaanxi mainly use seeds for propagation. Root reproduction, and the long-term single asexual reproduction method has led to serious pest problems, resulting in a decline in the quality and yield of medicinal materials, which has seriously affected the development of the Sichuan Salvia miltiorrhiza industry, and it is urgent to carry out the breeding of Sichuan Salvia miltiorrhiza

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HPLC method for measuring contents of 6 endogenous hormones in salvia miltiorrhiza bunge at the same time
  • HPLC method for measuring contents of 6 endogenous hormones in salvia miltiorrhiza bunge at the same time
  • HPLC method for measuring contents of 6 endogenous hormones in salvia miltiorrhiza bunge at the same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 The inventive method measures the endogenous hormone content of Salvia miltiorrhiza seed

[0047] 1. Preparation of the test solution

[0048] Grind 1g of frozen sample with liquid nitrogen (frozen sample is: fresh flowering buds collected at full flowering stage and stored in -80°C refrigerator), 0.2g of pvpp, 30mL of 80% pre-cooled methanol for 24 hours at 4°C to obtain the extract. The residue was grinded with 10 mL of 80% methanol and extracted twice, each time for 30 min. All the extracts were combined and centrifuged at -4°C at 10,000 r / min for 15 min. The supernatant was evaporated and concentrated under reduced pressure at 35°C to 40°C until there was no residual methanol (about 10ml remained).

[0049] Rinse the distillation flask twice with 10ml pH=8.0 phosphate buffer solution and 10ml petroleum ether in turn, decolorize and extract with equal volume of petroleum ether for 3 times, each time for 15min, and discard the ether phase. Adjust the pH...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
particle sizeaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides an HPLC method for measuring the contents of variousendogenous hormones in salvia miltiorrhiza bunge at the same time. The method comprises the following steps: a, preparing a test solution; b, preparing a reference solution; c, respectively injecting the test solution and the reference solution into a high performance liquid chromatograph to detect the solution; d, calculating the contents of various hormones in salvia miltiorrhiza bunge. The method can measure the contents of 6 endogenous hormones in salvia miltiorrhiza bunge at the same time, is accurate, reliable, simple and rapid, provides an effective theoretical basis for the research of mechanism of male sterile, and further lays a good foundation for developing the exogenous hormone regulation and variety optimization of stamen fertility of salvia miltiorrhiza bunge.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, in particular to an HPLC method for simultaneously measuring the contents of six endogenous hormones in Danshen. Background technique [0002] Salvia miltiorrhiza Bunge is a perennial herb of the Lamiaceae genus Salvia, mainly produced in Sichuan, Shandong, Henan, Shaanxi and other provinces. It has the functions of promoting blood circulation and promoting menstruation. It is mainly used for the treatment of cardiovascular and cerebrovascular diseases, coronary heart disease, liver cirrhosis and gynecological diseases. It is an ideal model plant for medicinal plants. [0003] In recent years, with the increase of clinical demand for salvia miltiorrhiza and its derivative products, the demand for raw medicinal materials of salvia miltiorrhiza in the domestic medicinal material market has also increased rapidly, and the planting area of ​​salvia miltiorrhiza is expanding year by year. ,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8634
Inventor 张利胡青青姜媛媛吴一超周永红杨程丁春邦苟丽琼吴忠旺佘启惠朱柏雨
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap