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Culture media for edible mycorrhizal fungi and symbiotic seedlings and synchronous culture method by utilizing culture media

A technology of mycorrhizal edible fungi and culture medium, which is applied in botany equipment and methods, application, cultivation, etc., and can solve the problems of complicated procedures for cultivating mycorrhizal edible fungus symbiotic seedlings, low survival rate, low mycorrhizal infection rate, etc. , to achieve the effect of increased mycelial survival rate, high survival rate and good growth activity

Active Publication Date: 2018-02-23
INST OF EDIBLE FUNGI SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies in the prior art and methods, to provide a culture medium and a method for synchronous cultivation of mycorrhizal edible fungus symbiotic seedlings, aiming to solve the problem of complex procedures for cultivating mycorrhizal edible fungal symbiotic seedlings in the prior art, and mycorrhizal symbiotic growth. Problems such as low infection rate and low survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of synchronous culture method that the mycorrhizal symbiosis seedling of masson pine and Poria cocos is synthetic, comprises the steps:

[0021] (1) Preparation of solid medium for mycelium expansion and propagation Weigh 20g of trehalose, 2g of yeast powder, 1g of peptone, 50ml of humus soil extract under the masson pine tree, 15g of agar, dissolve in purified water, and dilute to 1L, adjust the pH value to 5, put into test tubes or Petri dishes, and routinely sterilize.

[0022] (2) Preparation of liquid medium for cultivating mycelium for mycorrhizal symbiotic seedling inoculation Weigh 25g of trehalose, 6g of yeast powder, 2g of peptone, 50ml of humus soil extract under Pinus massoniana, dissolve in purified water, and set Make it into 1L, adjust the pH value to 5, put it into a Erlenmeyer flask, and perform routine sterilization.

[0023] (3) Poria cocos strain inoculum culture Inoculate Poria cocos hyphae on the solid medium prepared in (1) under aseptic c...

Embodiment 2

[0031] A synchronous culture method for the synthesis of mycorrhizal symbiotic seedlings of poplar and Tricholoma poplar, comprising the steps:

[0032] (1) Preparation of solid medium for mycelium expansion and propagation Weigh 24g of trehalose, 4g of yeast powder, 1.5g of peptone, 50ml of poplar humus extract, 18g of agar, dissolve in purified water, and constant volume into 1L, adjust the pH value to 5, put into test tubes or Petri dishes, and routinely sterilize.

[0033] (2) Preparation of liquid medium for cultivating mycelium for mycorrhizal symbiotic seedling inoculation Weigh 22g of trehalose, 3g of yeast powder, 1.2g of peptone, 50ml of poplar humus extract, dissolve in purified water, The volume was adjusted to 1L, the pH value was adjusted to 5, and it was filled into an Erlenmeyer flask, and routinely sterilized.

[0034] (3) Cultivation of the inoculum of Tricholoma poplaris Inoculate the mycelium of Tricholoma poplarus on the solid medium prepared in (1) under...

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Abstract

The invention discloses culture media for edible mycorrhizal fungi and symbiotic seedlingsand and a synchronous culture method by utilizing the culture media. The culture media for the edible mycorrhizal fungi and the symbiotic seedlings comprise an edible mycorrhizal fungus strain mycelium culture medium and an edible mycorrhizal fungus symbiosis seedling synthesis culture medium, and the ediblemycorrhizal fungus strain mycelium culture medium includes a solid culture medium used for expanding reproduction of the mycelium and a liquid culture medium used for culturing mycelium which is usedfor inoculation of the mycorrhizal symbiotic seedlings. The synchronous culture method for implementing edible mycorrhizal fungus symbiotic seedling synthesis by utilizing the above culture media comprises the following steps: (1) a cultivation method for the edible mycorrhizal fungus strain mycelium, (2) a cultivation method for the edible mycorrhizal fungus symbiotic seedlings. According to theinvention, cultivation of the edible mycorrhizal fungus mycelium is divided into two stages including solid culture and liquid culture, liquid fungus strains are adopted for inoculation, and meanwhilethe edible mycorrhizal fungus mycelium and symbiotic plants in the mycorrhizal seedling synthesis culture medium grow together, so that the infection ability of the mycelium is increased by 30% or more, the success rate of the inoculation is increased by 20%-30%, and the survival rates of symbiotic plant seedlings and the mycorrhizal fungus mycelium are both increased by 20% or more.

Description

technical field [0001] The technical field of the invention belongs to the technical field of cultivation and production of edible fungi, and in particular relates to the cultivation technology of symbiotic seedlings of mycorrhizal edible fungi. Background technique [0002] Mycorrhizal edible fungi such as matsutake, truffle, boletus, and poria cocos are a very important group of edible fungi with high edible value. During growth, they form symbiotic root systems with specific types of symbiotic plants and grow together. Mutual supply of growth substances, symbiotic coexistence. This type of edible fungus needs to live in symbiosis with living plants, accept the photosynthetic products of plants through the roots of living plants, and at the same time supply the nutrients it absorbs from the soil to the living plants, that is, form a plant-edible fungi symbiotic relationship. At present, there is no substantive breakthrough in the cultivation technology of artificially cul...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40A01G18/10
CPCC05B7/00C05G3/00C05C11/00C05D5/00C05F11/00C05F11/02
Inventor 周林郭尚王华南晓洁李艳婷
Owner INST OF EDIBLE FUNGI SHANXI ACAD OF AGRI SCI
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