Novel nucleic acid test micro-fluidic chip device

Abstract

The invention relates to a novel nucleic acid test micro-fluidic chip device for automatic extraction and purification detection of nucleic acid. The novel nucleic acid test micro-fluidic chip deviceis of a sealed cavity type structure and comprises a waste liquid chamber, a sample reagent chamber, a mixing chamber, a pre-amplifying chamber, an amplifying chamber, a biosensor and a signal outputport, wherein the waste liquid chamber, the sample reagent chamber, the mixing chamber, the pre-amplifying chamber and the amplifying chamber are connected through fluid microchannels, the biosensor and the signal output port are connected through electrical signals, and the fluid microchannels are distributed in the novel nucleic acid test micro-fluidic chip device in a three-dimensional and tubular mode; micropumps are respectively arranged in the waste liquid chamber, the sample reagent chamber, the mixing chamber and the pre-amplifying chamber, or flow dividing holes which are respectivelyconnected with external micropumps are formed in the waste liquid chamber, the sample reagent chamber, the mixing chamber and the pre-amplifying chamber. According to the novel nucleic acid test micro-fluidic chip device disclosed by the invention, pipelines can be cleaned by the micropumps, circular, repeated and discontinuous detection can be achieved, one item or a plurality of items of detection of one sample or a plurality of samples is achieved, and use cost is reduced.

Description

technical field [0001] The invention relates to a nucleic acid detection device, in particular to a novel nucleic acid detection microfluidic chip device for automatic nucleic acid extraction, purification and detection. Background technique [0002] Traditional nucleic acid detection methods include nucleic acid extraction, separation, and purification; the purified nucleic acid is further tested for genetic information such as PCR. Traditional nucleic acid detection is complex and has cumbersome steps. It needs to be operated in a specialized laboratory, and operators must pass professional training, which limits the promotion of nucleic acid detection. At the same time, traditional nucleic acid testing cannot detect multiple components at one time. If multiple items need to be tested, multiple parallel tests must be performed. In addition, since traditional nucleic acid detection is an open experimental operation, there are certain cross-contamination problems in manual ...

AI Technical Summary

Problems solved by technology

The existing microfluidic chip is only a single functional unit for amplification, elution, separation, etc.

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