Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polymerized peptide and gel having collagen-like structure

A technique of polymerizing peptides and gels, which is applied in the field of polymerizing peptides and can solve problems such as difficulty in using collagen

Active Publication Date: 2018-02-23
KOLA GEN PHARMA INC
View PDF8 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is substantially difficult to use natural collagen to obtain selectively active collagen with desired physiological activity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polymerized peptide and gel having collagen-like structure
  • Polymerized peptide and gel having collagen-like structure
  • Polymerized peptide and gel having collagen-like structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0354]

[0355] Materials and methods

[0356] peptide synthesis

[0357] In this example, peptides represented by SEQ ID NOs 1 to 22 described in Table 1 below were used for solid-phase synthesis.

[0358] Table 1

[0359] serial number

code name

amino acid sequence

1

YCC2

YCC(POG) 6 PRG (POG) 5 CCY

2

CC2

CC(POG) 6 PRG (POG) 5 CC

3

CC1

(POG) 6 PRG (POG) 5 CC

4

C2

C(POG) 6 PRG (POG) 5 C

5

CCC2

CCC(POG) 6 PRG (POG) 5 CCC

6

GGGCCC

CCCGGG(POG) 5 FOGERG (POG) 5 GGGCCC

7

CCCC2

CCCC(POG) 6 PRG (POG) 5 CCCC

8

Y2

Y(POG) 6 PRG (POG) 5 Y

9

YCC2-Scr

YCCGPPOGGOPOGPPOGGOPOGRPOGPPOGGOPOGCCY

10

CCC2-GPOGPR (short)

CCC(POG) 5 PRG (POG) 4 CCC

11

CCC2-GFOGER (short)

CCC(POG) 4 FOGERG (POG) 4 CCC

12

YCC2-GFOGER

YCC(POG) 5 FOGERG (POG) 5 CCY

13

YCC2-GVMGFO

Y...

Embodiment 2

[0379]

[0380] Confirmation of the triple helical structure confirmed by the conformation of the peptide

[0381] The structure of the peptide was confirmed by CD spectroscopy (circular dichroism disperser J-820, JASCO Corporation). Dissolve the peptide powder in degassed 0.05% TFA / H 2 O, thus preparing a 1 mg / ml solution. As a measurement sample, a solution obtained by heating at 80°C for 5 minutes and then allowing the solution to stand at 4°C overnight for renaturation was prepared. When the temperature is 4°C, 37°C, and 80°C, the length of the cuvette is 0.05cm, the sensitivity standard is (100mdeg), the measurement wavelength is 250-190nm, the data acquisition interval is 0.2nm, and the scanning speed is 50nm / min. The CD spectrum was measured under the conditions of 0.5 seconds, a bandwidth of 1 nm, and an accumulation count of 4 times. The obtained signal (θobs) is expressed as residual mean molar ellipticity (θmrw). θmrw is calculated by the following formula.

...

Embodiment 3

[0389]

[0390] A gel composed of polymerized peptides of polymerized collagen mimetic peptides was prepared by oxidatively crosslinking peptides having the above-mentioned triple helical structure with DMSO as an oxidizing agent. Polymerization by oxidative crosslinking was confirmed by measuring residual thiol groups.

[0391] Determination of Residual Thiol Groups in Oxidative Polymerization of Collagen Mimetic Peptides

[0392] A peptide solution with a concentration of 1.11% (w / v) was prepared using degassed water and placed in a PCR tube. After heating this at 80° C. for 5 minutes, it was left still at 4° C. overnight. DMSO was added to this solution to prepare a peptide solution having a final concentration of 10% DMSO and 1% (w / v) peptide. Filled with mineral oil to coat the surface. Mineral oil was removed from the peptide solution every time the measurement was performed to prepare a peptide solution for oxidation monitoring. Ellman's reagent, DTNB was prepared...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
degree of polymerizationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a polymerized peptide and a gel having a collagen-like structure. Although collagen is useful as a medicinal material, its use in humans involves the risks of allergy and zoonoses. Therefore, artificial collagens and the like are being studied as medicinal materials. However, typical artificial collagens cannot be considered completely sufficient, given their lack of physical strength, being denatured by heat, inability to be processed into a sheet, and the like, and thus being problematic in terms of practicality. In the present invention, a collagen-like peptide having a triple helical structure comprising triple strands of peptide chains including a plurality of cysteine (cys) residues is oxidized and cross-linked to prepare a polymerized peptide. This polymerized peptide may be processed into and used as a hydrogel and a thin-film sheet. In addition, by inserting a functional amino acid sequence present in biomacromolecules, particular physiological functions such as cell adhesiveness may be obtained. Further, the present invention provides: methods for preparing the polymerized peptide and the like; and uses thereof as compositions in cell cultures, wound coverings, regenerative medicinal materials, and research materials and the like by using the same.

Description

technical field [0001] The present invention relates to a polymeric peptide composed of collagen mimetic peptide, a gel containing the polymeric peptide, a polymeric peptide film, their production method and application. Background technique [0002] Collagen is a protein that forms a triple helical structure, and three chains of peptides (Xaa and Yaa are represented as arbitrary amino acid residues) have a primary structure amino acid sequence repeating -(Xaa-Yaa-Gly)- The general term for and is the main component of the extracellular matrix that exists between cells in animal tissues. Collagen is abundant in the combined tissues of animals and constitutes the skeleton structure of the tissues. It is told that there are 28 kinds of human collagen proteins. On the other hand, a motif with physiological activity is integrated in the amino acid sequence of the above-mentioned primary structure. For example, when tissue damage such as trauma occurs, since the collagen in the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K38/00A61L15/00A61P17/02C07K1/02C07K14/78C12M3/00
CPCA61K38/00C07K14/78A61P17/02A61L31/047A61L31/145A61L26/008A61L26/0047C07K14/00C12M3/00A61L15/00C07K1/02A61L15/325C08J3/075C12N5/0018
Inventor 小出隆规市濑慎一郎竹内俊吾能势博
Owner KOLA GEN PHARMA INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com