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Amniotic membrane mesenchymal stem cell release molecule (ASRM) as well as preparation method and application of ASRM

A sheep stem and amniotic membrane technology, applied in the field of bioengineering, can solve the problems of long doubling time and poor multi-differentiation potential of AMSCs cells

Inactive Publication Date: 2018-03-02
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to address the above problems, disclose a kind of goat stem extract and its preparation method and application, prepare goat stem extract and its preparation freeze-dried powder, solve the problems of long doubling time of AMSCs cells and poor multi-differentiation potential, It can qualitatively and quantitatively analyze the active ingredients of goat stems, improve the active ingredients in goat stems such as antioxidants and various growth factors, and prevent the active ingredients from degrading

Method used

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  • Amniotic membrane mesenchymal stem cell release molecule (ASRM) as well as preparation method and application of ASRM
  • Amniotic membrane mesenchymal stem cell release molecule (ASRM) as well as preparation method and application of ASRM
  • Amniotic membrane mesenchymal stem cell release molecule (ASRM) as well as preparation method and application of ASRM

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Experimental program
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Embodiment 1

[0055] A kind of preparation method of sheep dry element and freeze-dried powder thereof, comprises the following steps:

[0056] (1) AMSCs culture and expansion

[0057]Under sterile conditions, the amniotic membrane was removed from the placenta of normal full-term healthy infants delivered on the operating table, immediately soaked in α-MEM basal culture medium containing 200 U / ml penicillin and 200 μg / ml streptomycin and temporarily stored at 4°C. Primary cultures were performed within 12 hours. The amniotic membrane was taken out in a cell operation sterile bench, washed with phosphate buffered saline (PBS) containing 200 U / ml penicillin and 200 μg / ml streptomycin, removed blood, transferred to a 5ml EP tube and shredded with ophthalmic scissors. Then mix multiple tubes into a 50ml centrifuge tube, add 0.5mg / ml Trypsin (containing 0.02% EDTA) at a volume ratio of 1:10, shake in a sterile constant temperature shaker at 37°C at 200rpm / min for 30mins, centrifuge at 2000rpm ...

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Abstract

The invention discloses a sheep stem extract and its preparation method and application, and discloses a preparation method of its preparation freeze-dried powder. The sheep stem extract is obtained by separating and culturing amniotic mesenchymal stem cells from human placenta amniotic tissue, and using human platelets The lysate is cultured and expanded, and then the culture supernatant of human amniotic mesenchymal stem cells is collected, concentrated and collected, which solves the problems of long doubling time and poor multi-differentiation potential of AMSCs, and can qualitatively and Quantitative analysis can improve the active ingredients in sheep stems, such as antioxidants and various growth factors, and prevent the active ingredients from degrading. The results of animal experiments and clinical trials show that the sheep dry extract prepared by this technology can be used to delay aging, prevent and treat osteoporosis and skin aging, and promote skin wound healing.

Description

technical field [0001] The invention relates to the field of bioengineering, more specifically, to a preparation method of goat stem extract preparation and its application in anti-aging medicine. Background technique [0002] Recent studies have found that more than 80% of the therapeutic effects of human amniotic mesenchymal stem cells (Amniotic Membrane Mesenchymal Stem cells, AMSCs) transplantation are achieved through their paracrine effects. All the molecules released by stem cells are called the stem cell release molecule group, which can exert immune regulation, promote angiogenesis, anti-oxidation and anti-apoptosis, and promote tissue regeneration and repair through the paracrine pathway. Human amniotic mesenchymal stem cell release molecules (AmnioticMembrane Mesenchymal Stem cell Release Molecules, ASRM), referred to as sheep stem element. [0003] At present, although various foreign research institutions have confirmed the important position of stem cell relea...

Claims

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Application Information

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IPC IPC(8): A61K35/50A61K9/19A61K8/99C12N5/0775A61Q19/08A61P19/10A61P39/06A61P17/02
CPCA61K35/50A61K8/99A61K9/19A61Q19/08C12N5/0668C12N2500/84C12N2501/91
Inventor 苗登顺靳建亮胡梓轩陶建国王嵘
Owner NANJING MEDICAL UNIV
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