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Composite microbial agent capable of killing overwintered stem borers of rice and preparation method thereof

A technology of composite bacterial agent and microbial flora, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problems of simple insecticidal fungi or bacteria, and achieve easy promotion and soil improvement Physical and chemical condition, low cost effect

Inactive Publication Date: 2018-03-06
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the insecticide products on the market are mainly simple insecticidal fungi or bacteria, such as Beauveria bassiana, Metarhizium anisopliae, etc.

Method used

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  • Composite microbial agent capable of killing overwintered stem borers of rice and preparation method thereof
  • Composite microbial agent capable of killing overwintered stem borers of rice and preparation method thereof
  • Composite microbial agent capable of killing overwintered stem borers of rice and preparation method thereof

Examples

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Effect test

Embodiment 1

[0020] The invention provides a microbial compound bacterial agent for killing overwintering borers of rice and a preparation method thereof: the bacterial strain quality detection is performed on commercially purchased Trichoderma reesei, Aspergillus niger, Bacillus subtilis, Metarhizium anisopliae and Beauveria bassiana , the number of live bacteria detected is ≥ 10 billion / g, ≥ 10 billion / g, ≥ 20 billion / g, ≥ 10 billion / g, ≥ 15 billion / g qualified raw materials, respectively weighed 12.5kg of Trichoderma reesei , Aspergillus niger 25kg, Bacillus subtilis 20kg, Metarhizium anisopliae 5kg, Beauveria bassiana 10kg and rice bran powder (200 mesh) 27.5kg, then pour into the blender, add 10kg of sterile water, stir well, so that the bacteria agent is evenly adsorbed On top of the rice bran particles, it was finally placed in a drying oven and dried at a constant temperature of 45°C for 24 hours.

[0021] The small-scale detailed trial production process of the compound microbial ...

Embodiment 2

[0060] The invention provides a microbial compound bacterial agent for killing overwintering borers of rice and a preparation method thereof: the bacterial strain quality detection is performed on commercially purchased Trichoderma reesei, Aspergillus niger, Bacillus subtilis, Metarhizium anisopliae and Beauveria bassiana , the number of viable bacteria was screened to reach ≥ 20 billion / g, ≥ 10 billion / g, ≥ 10 billion / g, ≥ 10 billion / g, ≥ 15 billion / g qualified raw materials, respectively weighed 100 billion / g Trichoderma reesei kg, 10 kg of Aspergillus niger, 20 kg of Bacillus subtilis, 10 kg of Metarhizium anisopliae, 25 kg of Beauveria bassiana and 25 kg of rice bran powder (200 mesh), then pour into a blender, add 10 kg of sterile water, and stir well, In order to make the bacterial agent evenly adsorb on the rice bran particles, and finally place it in a drying oven to dry at a constant temperature of 45°C for 24 hours.

[0061] The present embodiment composite bacterial...

Embodiment 3

[0069] The invention provides a microbial composite bacterial agent for killing overwintering borers of rice and a preparation method thereof: the commercially purchased Trichoderma reesei, Aspergillus niger, and Beauveria bassiana are tested for strain quality, and the number of live bacteria screened out is respectively Qualified raw materials reaching ≥20 billion / g, ≥10 billion / g, and ≥15 billion / g, respectively weighed 25 kg of Trichoderma reesei, 25 kg of Aspergillus niger, 25 kg of Beauveria bassiana and 25 kg of rice bran powder (200 mesh) kg, then pour it into a blender, add 10 kg of sterile water, stir well, so that the bacterial agent is evenly adsorbed on the rice bran particles, and finally put it in a drying oven at a constant temperature of 45 ° C for 24 hours.

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Abstract

The invention provides a composite microbial agent capable of killing overwintered stem borers of rice and a preparation method thereof. The composite agent is prepared by mixing a microbial flora promoting rot of rice straw, a microbial flora causing death of infected overwintered stem borers, and an organic filling material, wherein the microbial flora promoting rot of rice straw comprises any one or a mixture of more than one of trichoderma reesei, aspergillus niger and bacillus subtilis; the microbial flora causing death of infected overwintered stem borers comprises one or a mixture of two at an optional ratio in beauveria bassiana and green muscardine fungus; and the organic filling material comprises any one or a mixture of more than one of bran, rice bran, rice hull powder, wheat straw meal and corn stalk meal. The composite microbial agent disclosed by the invention has dual insecticidal effects of destructing overwintering occasions causing death of stem borers and destructing infection causing death of stem borers and is low in cost, simple in application and easy to popularize, so that the overwintering death rate of stem borers can be improved by 60% or higher, the soil fertility can be increased while killing overwintered stem borers, and the composite microbial agent has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of microbial preparation manufacturing, in particular to a microbial composite bacterial preparation for killing overwintering rice borers and a preparation method thereof. Background technique [0002] Rice stem borer, including the rice stem borer, the three stem borers and the rice stem borer, is an important class of rice pests. The stem borers survive the winter as larvae in rice piles and straw. In recent years, mechanized harvesting has become popular. After mechanized harvesting, rice straw is no longer used as fuel or animal feed, but the straw is directly returned to the field when harvesting. Therefore, the overwintering places of borers are basically limited to rice piles and scattered straw in the field. . Rice piles and straws play an important role in the survival of overwintering borers, including: providing a suitable overwintering space to reduce the risk of freezing of insects; providing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/00A01P7/04C12N1/14C12N1/20C12R1/885C12R1/685C12R1/125C12R1/645
CPCA01N63/00A01N63/30C12N1/14C12N1/20
Inventor 王永模刘樾郭昊
Owner HUAZHONG AGRI UNIV
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