Culture medium for multiplying test-tube seedlings based on somatic embryos of pear tree

Abstract

The invention discloses a culture medium for multiplying test-tube seedlings based on somatic embryos of a pear tree. The culture medium is prepared from the following raw materials in parts by weight: 14-26 parts of an organosilicone modified starch water emulsion, 10-20 parts of 1 / 2 modified SH, 20-30 parts of sucrose, 20-30 parts of non-ionic polyacrylamide, 5-11 parts of graft modified soybeanprotein, 15-25 parts of alternaria tenuissima protein, 11-19 parts of carbonase, 11-19 parts of sodium p-nitrophenolate, 6-8.4 parts of eugenol, 1.5-6.5 parts of paclobutrazol, 1-3 parts of bacilluscereus, 3-6 parts of a beta-compound detoxification enzyme, 3-5 parts of bacillus natto, 3-5 parts of 6-benzyl aminopurine, 0.5-1.0 part of naphthylacetic acid and 3-6 parts of 2, 4-D. Through optimization of the components of the culture medium, the survival rate of transplanted plants is greatly increased, the incidence of diseases of the seedlings is reduced and the seedlings grow healthily andstrongly.

Description

technical field [0001] The invention relates to the field of pear planting, in particular to a test-tube seedling growth medium for somatic embryos of pear trees. Background technique [0002] Pear trees belong to the genus Pyrus in the family Rosaceae. They are perennial deciduous fruit trees and trees. They are widely distributed around the world. There are about 35 native species in the world. They are wildly distributed in Europe, Asia and Africa. They are divided into two categories: Oriental pear and Western pear. kind. China's pear cultivation area and output are second only to apples. [0003] Pear tree is a kind of tree species with high development value. At present, the propagation method usually adopted is cutting propagation, and this propagation method is susceptible to fungal diseases such as leaf spot. It is also vulnerable to aphids in spring and summer. The virus Seriously, the leaf mottling is easy to fall off, and the degree of improved variety is reduc...

AI Technical Summary

Problems solved by technology

[0003] Pear tree is a kind of tree species with high development value. At present, the commonly used propagation method is cutting propagation, and this propagation method is susceptible to fungal diseases such as leaf spot. It is also vulnerable to aphids in spring and summer. The virus Seriously, the leaf mottling is easy to fall off, and the degree of improved variety is reduced; and the use of inferior seedlings leads to the loss of the inherent good characteristics of pear trees, which restricts the development and application of this tree species to a certain extent. Therefore, selection, breeding Excellent pear tree strains become a very prominent production problem