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Method of measuring content of effective components in bacopa monnieri total saponin extract

A technology of Bacopa monnieri and active ingredients, which is applied in the field of determination of active ingredients of traditional Chinese medicines

Inactive Publication Date: 2018-03-13
山东颐正达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the assay method for the above-mentioned active ingredients has not been reported so far. In order to ensure the quality stability of the total saponins extract of Bacopa monniera, corresponding detection methods and quality control standards should be established.

Method used

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  • Method of measuring content of effective components in bacopa monnieri total saponin extract
  • Method of measuring content of effective components in bacopa monnieri total saponin extract
  • Method of measuring content of effective components in bacopa monnieri total saponin extract

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Embodiment 1

[0056] Preparation of Total Saponins Extract from Bacopa monnieri

[0057] Take 5kg of Bacopa monniera medicinal material and place it in a multifunctional extraction tank, add 50kg of 80% ethanol each time, extract 3 times, each time for 1h, combine the extracts, concentrate (-0.6Bar pressure, 60°C) until there is no alcohol smell , add 95% ethanol to adjust the alcohol content to 30%, centrifuge, filter with suction, and the content of the medicinal material is 0.311g·mL -1 of the sample solution. Take the HP-20 macroporous resin that has been pretreated (washed with 95% ethanol 10 times the weight of the resin), and pack it into a column by wet method (diameter-to-height ratio 1:10), with a column volume of 500 mL. Take 800mL of sample solution (0.5kg medicinal material / L resin), adjust the pH value to 2.0 with concentrated hydrochloric acid, and use 0.5BV·h -1 Load the sample at a flow rate until it is complete, then use 2BV of 30% ethanol (pH 2.0) and 5BV of 40% ethanol...

Embodiment 2a

[0060] The Bacopa monnieri total saponins extract that embodiment 1 makes adopts AgelaPromsil C 18 (5μm, 4.6mm×250mm) chromatographic column, with 32.5% acetonitrile / 67.5% 0.2% phosphoric acid water (NaOH adjusted pH=3) as mobile phase, 1ml / min flow rate, 30°C column temperature, 205nm detection wavelength, elution 45 minute. The purity of the total saponins of the total saponins extract from Bacopa monniera was measured to be 55.67%, and the transfer rate of the total saponins was 78.29%.

Embodiment 2b

[0062] The total saponins extract of Bacopa monniera prepared in Example 1 adopts Shiseido Capcell-C18 (5 μm, 4.6mm × 250mm) chromatographic column, with 32.5% acetonitrile / 67.5% 0.2% phosphoric acid water (NaOH adjusts pH=3) as flow Phase, 1ml / min flow rate, 30°C column temperature, 205nm detection wavelength, elution for 45 minutes. The purity of the total saponins of the total saponins extract from Bacopa monniera was measured to be 55.62%, and the transfer rate of the total saponins was 77.97%.

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Abstract

The invention provides a method of measuring the content of effective components in a bacopa monnieri total saponin extract. According to the method, high performance liquid chromatography (HPLC) is used to measure the content of effective components in a bacopa monnieri total saponin extract. According to the method, a reference solution and a test solution are prepared; an AgelaProms chromatographic column (C18, 5[mu]m, 4.6 mm*250 mm) is adopted; 32.5% acetonitrile / 67.5% phosphoric acid with a pH value of 3 (adjusted by NaOH) is taken as the mobile phase; and elution is carried out for 45 minutes to simultaneously measure contents of five saponins under following conditions: flowing speed: 1 mL / min, column temperature: 30 DEG C, and detection wavelength: 205 nm. The provided method analyzes the effective components in a bacopa monnieri total saponin extract, establishes quality standards of the effective components in a bacopa monnieri total saponin extract, guarantees the stable quality of bacopa monnieri total saponin extract preparation, and has a great application value.

Description

technical field [0001] The invention relates to a method for determining the effective components of traditional Chinese medicines, in particular to a method for determining the content of the active components in total saponins of bacopa monniera. Background technique [0002] Bacopa monnieri is the whole herb of Bacopamonnieri (L.) Wettst, a plant of the family Scrophulariaceae, also known as bacopa monnieri. It has the effects of clearing away heat and cooling blood, detoxifying and reducing swelling. The main active part of Bacopa monnieri is its saponin active ingredient, five saponin compounds such as Bacopaside I, (dammarane-type triterpene saponin compound Bacopaside I (BM28), Bacoside A 3 (BM37), Bacopaside II (BM29), Bacopasaponin C isomer (BM35i) and Bacopasaponin C (BM35), the structural formulas of the five saponins are as follows: [0003] [0004] [0005] However, there is no report on the determination method of the above-mentioned active ingredients...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张卫东孙青
Owner 山东颐正达医药科技有限公司
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