Glycine creep compound feed for piglets
A technique of combining glycine and trough, which is applied in the biological field to achieve the effects of reducing usage, preventing the harm of drug-resistant bacteria, and reducing pollution
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Embodiment 1
[0032] Glycine and glucose increase antibiotic access to bacteria
[0033] Bacterial death was related to the amount of antibiotics that got inside the bacteria. In order to study the effects of glycine and glucose on promoting antibiotics to enter the bacteria, a single colony of Edwardsiella tarda EIB202 was picked from the LB plate and inoculated in 5 mL of LB medium, and cultured at 200 rpm at 30°C for 24 hours to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL was used as a test sample in a test tube for later use. The experiment was divided into 5 groups, of which 2 groups were control groups, without adding any substances and adding antibiotics; the other 3 groups were experimental...
Embodiment 2
[0035] Glycine and glucose increase susceptibility of multiple bacteria to kanamycin antibiotic
[0036] Pick a variety of bacteria: Staphylococcus aureus (S.aureus), Pseudomonas aeruginosa (Pseudomonas aeruginosa, P.aeruginosa), Escherichia coli clinical drug-resistant bacteria (Y15), Vibrio alginolyticus (V.alginolyticus) Put the single clone into 100ml LB liquid medium, culture at 200rpm at 37°C or 30°C for 16h to reach saturation. Collect 20ml of bacterial liquid, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with an equal volume of 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD of the bacterial solution to 0.2, and divide Put 5mL in a test tube, add kanamycin as the control group, add 20mM glycine, 10mM glucose, 20mM glycine and 10mM glucose respectively as the experimental group, incubate at 37°C or 30°C at 200rpm for 6h, take 100μL of bacteria Colony counting was carr...
Embodiment 3
[0038] Glycine and / or glucose increase the susceptibility of Escherichia coli and its clinical drug-resistant bacteria to oxytetracycline
[0039] (1) Glycine and / or glucose increase the sensitivity of Escherichia coli to oxytetracycline
[0040] Preparation of Escherichia coli test samples: Pick a single colony of Escherichia coli from the LB plate and inoculate it in 5 ml of LB medium, culture at 200 rpm at 37°C for 16 hours to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL in a test tube for later use.
[0041] The prepared samples were divided into 5 groups, of which 2 groups were the control group, without adding any substances and oxytetracycline; the other 3 groups were the experi...
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