Crystal violet degrading bacterium and application thereof
A technology of crystal violet and degrading bacteria, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., to achieve the effect of good operability, no secondary pollution and simple method
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[0030] Domestication and isolation of crystal violet degrading bacteria
[0031] Take 3.0 g of the polluted soil sample that has been dyed for a long time, put it in 100 mL of inorganic salt medium with an initial crystal violet concentration of 5 mg / L, and culture it with shaking at 30 °C and 130 r / min; observe the decolorization of crystal violet every 2 days, Take 5ml of the bacterial suspension with the best decolorization effect and transfer it to fresh culture medium containing crystal violet, and gradually increase the mass concentration of crystal violet to 10, 20, 40, 60, 80, 100 mg / L in turn, carry out strain acclimatization, and determine the crystal violet The degradation and decolorization effect of violet; the domesticated bacterial suspension that can decolorize 100mg / L crystal violet solution is coated on a solid dye plate containing 30mg / L crystal violet, and the separation of crystal violet degrading bacteria is carried out, and the strain identification is ca...
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