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ErbB2 antigen epitope peptide bonded with E9 antibody
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An antigen epitope and antigen technology, applied in the field of biomedicine, can solve problems such as limiting the scope of application
Active Publication Date: 2018-04-06
HEFEI HANKEMAB BIOTECH CO LTD
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Problems solved by technology
However, with its widespread use, drug resistance also appears, limiting its application range
Moreover, the domestic price of 440mg Trastuzumab exceeds RMB 25,000, and the cost of each course of treatment exceeds RMB 200,000
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Embodiment 1
[0083] Example 1, Analysis of antigenic epitopes in the scFv-ECD complex
[0084] This example analyzes the scFv of the E9 antibody (that is, the single-chain antibody formed by connecting the light chain variable region and the heavy chain variable region of E9, referred to as E9scFv) and the ECD region of the ErbB2 protein (ErbB2ECD, sequence 6 in the sequence listing) The formed scFv-ECD complex determined the binding region of E9 antibody and ErbB2ECD and the corresponding epitope.
[0085] 1. Preparation of scFv-ECD complex
[0086] Both E9scFv and ErbB2ECD express an Fc fragment of IgG1 in series at their carboxy-terminal by genetic engineering methods, and add an enterokinase restriction site before the Fc fragments of the two (the resulting fusion proteins are respectively designated as E9scFv-Fc and ErbB2ECD -Fc) for preparation. The fusion proteins were all secreted and expressed by the eukaryotic expression system CHO-K1 cell line, purified and mixed with enteroki...
Embodiment 2
[0161] Example 2, E9 antibody has the effect of inhibiting tumor cell growth
[0162] The test cells are: OE19 (Nanjing Kebo Biotechnology Co., Ltd.)
[0163] The antibodies or antibody combinations to be tested are: E9, Pertuzumab, Herceptin, Pertuzumab+Herceptin (mass ratio 1:1), E9+Herceptin (mass ratio 1:1).
[0164] Specific steps are as follows:
[0165] 1. Trypsinize the cells in logarithmic growth phase, add culture medium to make cell suspension;
[0166] 2. 3000-20000ug antibody / 100μl per well, add cells into 96-well plate, and culture for 24hr;
[0167] 3. From the highest concentration, add 100 μl of the test product diluted >9 concentrations by 2-4 times to each well, 3-6 parallel wells for each concentration, and use the medium as the control; the combined drugregimen is two antibodies according to 1: The mass ratio of 1 is mixed with the drug, and the dosage of each antibody is the same as that of the antibody single drug;
[0168] 4. After 72 hours, add 20...
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Abstract
The invention discloses an ErbB2 antigenepitopepeptide bonded with an E9 antibody. The ErbB2 antigenepitopepeptide disclosed by the invention is an antigenepitopepeptide respectively named as A1and / or B1, wherein A1 contains amino acid residues as follows: Arg at a site 136 of a sequence 6 in a sequence table, Pro at a site 138, Cys at a site 141, Tyr at a site 142 and Asp at a site 144; and B2 contains amino acid residues as follows: Asn at a site 141 of the sequence 6 in the sequence table, Arg at a site 167, Cys at a site 171, His at a site 172 and Glu at a site 186. Compared with abonding region between chA21 and ErbB2, an ErbB2 antibody, antigen epitope peptide bonded with the ErbB2 antibody and antigen epitope have the advantages that the bonding sequences between E9 and ErbB2 are reduced, and the preparation of new ErbB2 antibody is promoted.
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