Composite wall breaking treatment method of ganoderma lucidum spore powder

A technology of Ganoderma lucidum spore powder and processing method, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and the dissolution of microorganisms, and can solve the problems of difficult control of the germination conditions of the activated spore germination method, long wall breaking period of the bacterial dissolution method, and poor germination. Deal with problems such as long time to achieve the effect of reducing mechanical impurities and heavy metal pollution, reducing mechanical pollution and heavy metal pollution, and shortening the treatment time

Active Publication Date: 2018-04-10
THE QUARTERMASTER RES INST OF THE GENERAL LOGISTICS DEPT OF THE CPLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological wall-breaking methods mainly include enzymatic hydrolysis, bacteriolysis, and spore germination activation. The main problems of biological wall-breaking at this stage are: the enzymatic hydrolysis time of ordinary enzymatic hydrolysis is long, the wall-breaking rate is low, and the residues in the product are removed. It is difficult to use enzymes; the bacteriolysis method has a long period of breaking the wall and is easily contaminated by bacteria; the germination conditions of the activated spore germination method are not easy to control, the germination treatment time is long, it is easy to be contaminated by bacteria, and the wall breaking rate is not high, etc.

Method used

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  • Composite wall breaking treatment method of ganoderma lucidum spore powder
  • Composite wall breaking treatment method of ganoderma lucidum spore powder
  • Composite wall breaking treatment method of ganoderma lucidum spore powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Sterilize 0.2g of Ganoderma lucidum spore powder at 105°C for 30 minutes, keep it warm in a sterilizer for 8 hours, and then dry it.

[0058] 2. Prepare the liquid submerged culture fermentation broth of Ganoderma lucidum mycelium:

[0059] (1) Seed medium: glucose 2g, yeast powder 0.5g, KH 2 PO 4 0.1g, MgSO 4 ·H 2 O 0.1g, water 100mL, pH 6.0.

[0060] (2) Fermentation medium: glucose 15g, yeast powder 7.5g, KH 2 PO 4 1g, MgSO 4 ·H 2 O 0.5g, water 500mL, pH6.0.

[0061] (3) Preparation method: the ganoderma lucidum mycelium was shaken in a seed medium at 25° C. for 3 days, then transferred to a fermentation medium with 15% inoculum size and cultured at 28° C. for 5 days.

[0062] 3. Under aseptic conditions, take 1.2mL of fermentation liquid and add it to 0.2g of sterilized Ganoderma lucidum spore powder, and mix well.

[0063] 4. Incubate statically for 3 days at 25°C in the dark.

[0064] 5. Place the processed spore powder in a freeze dryer for drying. ...

Embodiment 2

[0079] 1. Sterilize 25g of Ganoderma lucidum spore powder at 105°C for 20 minutes, keep it in a sterilizing pot for 15 hours, and then dry it. Figure 4 (A) shown.

[0080] 2. Prepare the liquid submerged culture fermentation broth of Ganoderma lucidum mycelium:

[0081] (1) Seed medium: glucose 2g, yeast powder 0.5g, KH 2 PO 4 0.1g, MgSO 4 ·H 2 O 0.1g, water 100mL, pH 6.0.

[0082] (2) Fermentation medium: glucose 15g, yeast powder 7.5g, KH 2 PO 4 1g, MgSO 4 ·H 2 O 0.5g, water 500mL, pH6.0.

[0083] (3) Preparation method: the ganoderma lucidum mycelium was shaken in a seed medium at 28° C. for 3 days, then transferred to a fermentation medium with 15% inoculum size and cultured at 25° C. for 5 days.

[0084] 3. Under sterile conditions, take 150ml of fermentation broth and add it to 25g of sterilized Ganoderma lucidum spore powder, mix well, as Figure 4 (B) shown.

[0085] 4. 25°C, constant temperature germination culture under dark conditions for 2 days, such a...

Embodiment 3

[0094] 1. Sterilize 75g of Ganoderma lucidum spore powder at 121°C for 10 minutes, keep it warm in a sterilizer for 8 hours, and then dry it.

[0095] 2. Prepare the liquid submerged culture fermentation broth of Ganoderma lucidum mycelium:

[0096] (1) Seed medium: glucose 1.5g, yeast powder 0.75g, KH 2 PO 4 0.2g, MgSO 4 ·H 2 O 0.1g, water 100mL, pH 5.0.

[0097] (2) Fermentation medium: 500 mL of wort medium of 11 ° Brix, pH natural.

[0098] (3) Preparation method: put the ganoderma lucidum mycelium in the liquid seed culture medium at 26°C

[0099] After shaking for 3 days, transfer to liquid fermentation medium according to 12% inoculum size and culture at 26°C for 4 days.

[0100] 3. Under sterile conditions, take 150ml of fermented broth filtrate and add it to 75g of sterilized Ganoderma lucidum spore powder, and mix well.

[0101] 4. 25°C, constant temperature germination culture in the dark for 2 days.

[0102] 5. Place the treated spore powder in a spray drye...

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Abstract

The invention discloses a composite wall breaking treatment method of ganoderma lucidum spore powder. The composite wall breaking treatment method comprises following steps: under a standing state, adopting ganoderma lucidum mycelia liquid submerged culture fermentation broth or filtrate thereof to carry out infiltration type germination culture and spore wall enzymatic hydrolysis on ganoderma lucidum spore powder; after infiltration type germination culture and spore wall enzymatic hydrolysis, drying the ganoderma lucidum spore powder, and finally carrying out mechanical wall breaking. Afterganoderma lucidum spore powder is processed by germination activation and spore wall enzymatic hydrolysis, a liquid nitrogen low temperature embrittling treatment is carried out, the mechanical wall breaking time is greatly reduced, the active components are better preserved, the mechanical impurities and heavy metal pollution are reduced effectively; the pollution of foreign enzymes is avoided; moreover, the wall breaking rate of ganoderma lucidum spore powder is improved effectively, and green and efficient wall breaking of ganoderma lucidum spore powder is realized.

Description

technical field [0001] The invention relates to a wall-breaking treatment method of ganoderma lucidum spore powder, in particular to a composite wall-breaking treatment method of ganoderma spore powder. Background technique [0002] Ganoderma Lucidum (Ganoderma Lucidum) is a precious medicinal material with a long history in my country, which has the functions of protecting the liver, strengthening the brain and fitness. Ganoderma Lucidum spores, as the reproductive cells of Ganoderma lucidum, not only contain all the active substances of Ganoderma lucidum, but also have higher active ingredients, medicinal value and health care value than fruiting bodies of Ganoderma lucidum. The spores of Ganoderma lucidum are light brown to yellowish brown, containing an oil droplet, ovoid, with a diameter of about (8.5-11.2) μm×(5.2-6.9) μm[7], the top is often truncated, double-walled, and the inner spore wall has Protruding spines, exosporium smooth, colorless. [0003] Polysaccharid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12R1/645
CPCC12N1/06C12N1/066
Inventor 郝利民董扬江庆伍鲁吉珂刘宇琪聂琳然郑志强马天娇
Owner THE QUARTERMASTER RES INST OF THE GENERAL LOGISTICS DEPT OF THE CPLA
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