A kind of production method of kiwifruit canker-resistant two-way virus-free large seedlings

A production method and canker technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of canker risk, seedling carrying, non-disease resistance, etc., to achieve canker control, strong root vigor, Strong anti-ulcer effect

Active Publication Date: 2020-01-14
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of kiwifruit anti-canker two-way detoxification large seedling production method, to solve the traditional production technology of kiwifruit seedlings "cross-infection of scion in epidemic areas leads to risk of canker disease in seedlings, rootstock and scion are not disease-resistant at the same time, resulting in post-infection damage." Garden" and other issues

Method used

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  • A kind of production method of kiwifruit canker-resistant two-way virus-free large seedlings
  • A kind of production method of kiwifruit canker-resistant two-way virus-free large seedlings
  • A kind of production method of kiwifruit canker-resistant two-way virus-free large seedlings

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0082] Example 1 - Production and application of 'Hongyang' canker-resistant seedlings

[0083] Production of 'Kr-1' rootstock seedlings: The disease-resistant rootstock 'Kr-1' was obtained through seed breeding, and was identified as a material with high canker resistance by the above method (see the above screening and identification of kiwifruit canker-resistant rootstocks), and was compared with 'Kr-1' Hongyang' has good affinity; then produce tissue culture seedlings through tissue culture detoxification, the specific method is as follows:

[0084] Take 'Kr-1' new shoot tip <5mm shoot tip, wash with sterile water, disinfect with 75% alcohol and 0.1% mercury chloride, use MS as the basal medium to induce callus, containing 2mg / L of 6- BA and 0.2mg / L NAA, after dark culture for 5-7 days, light culture for 25-35 days; the subculture medium is based on MS medium, containing 2mg / L 6-BA and 0.3mg / L NAA; Rooting medium is based on 1 / 2MS medium, containing 0.6mg / L of IBA. Other...

example 2

[0094]Example 2 - Production and application of 'Hongsheng' canker-resistant seedlings

[0095] Production of 'Kr-1' rootstock seedlings: The same disease-resistant rootstock 'Kr-1' as that of 'Hongyang' was used in the production of 'Hongsheng' variety seedlings. —The production and application of 'Hongyang' anti-ulcer seedlings" are exactly the same.

[0096] 'Hongsheng' non-toxic seedling production: The original material of 'Hongsheng' was taken from the original breeding place of Sanchuan Breeding Base in Cangxi County, Sichuan Province in 2010. The stem tip material of 'Hongsheng' was used. Subculture and rooting, etc., to produce 'Hongsheng' non-toxic tissue culture seedlings, the specific methods are as follows:

[0097] Take shoot tips <5 mm from the top of shoots, wash them with sterile water, disinfect them with 75% alcohol and 0.1% mercury chloride, and use MS as the basal medium to induce callus, containing 1.5mg / L of 6-BA and 0.5mg / L of ZT, cultured in the dar...

example 3

[0101] Example 3 - Production of 'Jinyan' anti-canker seedlings and renewal application of old orchards

[0102] Production of 'Hr-1' rootstock seedlings: The disease-resistant rootstock 'Hr-1' was obtained through seed breeding, and was identified as a material with high canker resistance by the above method (see screening and identification of kiwifruit canker-resistant rootstocks), and it was compared with 'Gold Brilliant 'has good affinity; then produce tissue culture seedlings by tissue culture detoxification, the specific method is as follows:

[0103] Take shoot tips <5 mm from the top of shoots, wash them with sterile water, disinfect them with 75% alcohol and 0.1% mercury chloride, and use MS as the basal medium to induce callus, containing 1.5mg / L of 6-BA and 0.5mg / L of KT, cultured in the dark for 5-7 days, and cultured in the light for 20-30 days; the subculture medium was based on MS medium, containing 1mg / L of 6-BA and 0.5mg / L of NAA; the rooting medium was 1 / 2...

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Abstract

The invention discloses a method for producing canker-resistant bi-directional detoxification large seedlings of kiwifruit. The method comprises the steps of: firstly performing detoxification and tissue culture on stem tips of canker-resistant kiwifruit rootstocks to produce special rootstock seedlings which reach a certain standard; establishing a non-canker cutting orchard for a scion variety through the detoxification and tissue culture techniques, and performing isolation planting and periodic inspection so as to ensured that the scion variety in the cutting orchard does not carry pathogens of kiwifruit canker; performing grafting when the rootstocks and scions are tested without canker infection, and performing cultivation to obtain the canker-resistant bi-directional detoxificationlarge seedlings. In order to solve the present serious problem that most of red meat kiwifruit and yellow meat kiwifruit are susceptible to canker, the method aims to solve the problem of cross-infection of infected kiwifruit seedlings fundamentally, so that the need for the canker-resistant seedlings is met during new building of red meat and yellow meat kiwifruit orchards or renewal of old orchards; the method is a best scheme to solve the canker outbreak of a main variety and destruction of the orchards in the current kiwifruit production, so that the method is applied to large-scale commercial use in production.

Description

technical field [0001] The invention belongs to the technical field of kiwi fruit cultivation, and in particular relates to a production method of kiwi fruit two-way detoxification-resistant canker-resistant seedlings, which is applied to the production of kiwi fruit anti-canker disease seedlings. Background technique [0002] As a unique functional fruit, kiwi fruit has grown rapidly in China's cultivation area and consumption in the past 10 years, and its importance is still rising, surpassing other traditional minor fruits. China is also the main distribution center and diversity center of kiwifruit. Canker disease is a vicious infectious bacterial disease caused by Pseudomonas syringaepv. It has the characteristics of wide occurrence range, rapid spread, strong pathogenicity and difficult control, and it is very easy to cause large-scale tree death in a short period of time. Under the current status of seedling breeding and introduction of horticultural crops in my cou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00A01G2/30A01H4/00
CPCA01G17/005A01H4/001
Inventor 王彦昌满玉萍何仕松李黎吴世权雷瑞高祖平潘慧钟彩虹
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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