A kind of production method of kiwifruit canker-resistant two-way virus-free large seedlings
A production method and canker technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of canker risk, seedling carrying, non-disease resistance, etc., to achieve canker control, strong root vigor, Strong anti-ulcer effect
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example 1
[0082] Example 1 - Production and application of 'Hongyang' canker-resistant seedlings
[0083] Production of 'Kr-1' rootstock seedlings: The disease-resistant rootstock 'Kr-1' was obtained through seed breeding, and was identified as a material with high canker resistance by the above method (see the above screening and identification of kiwifruit canker-resistant rootstocks), and was compared with 'Kr-1' Hongyang' has good affinity; then produce tissue culture seedlings through tissue culture detoxification, the specific method is as follows:
[0084] Take 'Kr-1' new shoot tip <5mm shoot tip, wash with sterile water, disinfect with 75% alcohol and 0.1% mercury chloride, use MS as the basal medium to induce callus, containing 2mg / L of 6- BA and 0.2mg / L NAA, after dark culture for 5-7 days, light culture for 25-35 days; the subculture medium is based on MS medium, containing 2mg / L 6-BA and 0.3mg / L NAA; Rooting medium is based on 1 / 2MS medium, containing 0.6mg / L of IBA. Other...
example 2
[0094]Example 2 - Production and application of 'Hongsheng' canker-resistant seedlings
[0095] Production of 'Kr-1' rootstock seedlings: The same disease-resistant rootstock 'Kr-1' as that of 'Hongyang' was used in the production of 'Hongsheng' variety seedlings. —The production and application of 'Hongyang' anti-ulcer seedlings" are exactly the same.
[0096] 'Hongsheng' non-toxic seedling production: The original material of 'Hongsheng' was taken from the original breeding place of Sanchuan Breeding Base in Cangxi County, Sichuan Province in 2010. The stem tip material of 'Hongsheng' was used. Subculture and rooting, etc., to produce 'Hongsheng' non-toxic tissue culture seedlings, the specific methods are as follows:
[0097] Take shoot tips <5 mm from the top of shoots, wash them with sterile water, disinfect them with 75% alcohol and 0.1% mercury chloride, and use MS as the basal medium to induce callus, containing 1.5mg / L of 6-BA and 0.5mg / L of ZT, cultured in the dar...
example 3
[0101] Example 3 - Production of 'Jinyan' anti-canker seedlings and renewal application of old orchards
[0102] Production of 'Hr-1' rootstock seedlings: The disease-resistant rootstock 'Hr-1' was obtained through seed breeding, and was identified as a material with high canker resistance by the above method (see screening and identification of kiwifruit canker-resistant rootstocks), and it was compared with 'Gold Brilliant 'has good affinity; then produce tissue culture seedlings by tissue culture detoxification, the specific method is as follows:
[0103] Take shoot tips <5 mm from the top of shoots, wash them with sterile water, disinfect them with 75% alcohol and 0.1% mercury chloride, and use MS as the basal medium to induce callus, containing 1.5mg / L of 6-BA and 0.5mg / L of KT, cultured in the dark for 5-7 days, and cultured in the light for 20-30 days; the subculture medium was based on MS medium, containing 1mg / L of 6-BA and 0.5mg / L of NAA; the rooting medium was 1 / 2...
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