Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of nano-medicine for controllable photodynamic therapy and preparation method thereof

A technology of photodynamic therapy and nano-medicine, which is applied in the field of biomedicine, can solve the problem of high dark toxicity of hematoporphyrin, and achieve the effect of improving the therapeutic effect, enhancing the therapeutic effect, and improving the solubility

Active Publication Date: 2020-11-10
FUZHOU UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a controllable preparation method and application of nano drug CPHI with low dark toxicity. Using the function of ICG switch, it not only solves the problem of high dark toxicity of hematoporphyrin, but also realizes controllable Combination of photodynamic therapy and photothermal therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of nano-medicine for controllable photodynamic therapy and preparation method thereof
  • A kind of nano-medicine for controllable photodynamic therapy and preparation method thereof
  • A kind of nano-medicine for controllable photodynamic therapy and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Weigh β-CD (126.4 mg,) and CDI (180.56 mg) was dissolved in 7 mL of DMSO. After stirring the reaction sufficiently 6 h, to the solution was added dropwise a solution of fifth generation polyamide - amine dendrimers (G5 PAMAM) (115.9 mg) in DMSO (3 mL), stirred for 3 days at room temperature. After completion of the reaction, the reaction solution was transferred to 8000-14000 Da molecular weight cutoff dialysis bag, the water in the secondary dialyzed for 3 days. Finally lyophilized to give a white powdery product (CP), stored at -20 ℃. Deuterated DMSO was used as 1 H-NMR spectrum of the test solvent, from figure 1 Found that cyclodextrin has been successfully conjugated to PAMAM.

Embodiment 2

[0043] Take CP, ICG, HP aqueous ultraviolet absorption measurement, such as figure 2 Shown, HP at 374nm, 500-620 nm with a UV absorption peak at a, ICG has characteristic UV absorption peak at 778 nm, CP no UV absorption at 300-900 nm. Measuring CP, ICG, HP fluorescence intensity at an excitation wavelength such as 401nm image 3 HP shown in 616nm, 666nm HP has a characteristic peak, while no fluorescence CP and CPI.

Embodiment 3

[0045] Weigh 10 mg CP dissolved in 8mL DMSO, the ICG (2mg, 1mL DMSO) and HP (0.2mg, 1mL DMSO) was added dropwise to the CP solution, stirred for 3 days at room temperature in the dark. The reaction solution was dialyzed for 3 days in a secondary water, and lyophilized to yield CPHI10. Wherein, ICG encapsulation efficiency and the loading rate was 15.4% ± 0.63% and 6.16% ± 0.03%, respectively, HP encapsulation efficiency and the loading rate was 82.5% ± 0.25% and 3.30% ± 0.64%, respectively.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses polyamidoamine-dendrimers (PAMAM-CD) / HP(haematoporphyrin) / ICG(indocyanine green) nanometer medicine with controllable photodynamic treatment effects, and a preparation method thereof. The nanometer medicine is characterized in that the HP and the ICG are coated and loaded into PAMAM-CD; the ICG is used as a switch of the HP; the fluorescence of the HP is quenched by ICG; meanwhile, the photodynamic treatment is switched off. When the nanometer medicine is exposed in 808nm laser, the ICG is decomposed; the HP fluorescence is restored; the photodynamic treatment is opened; meanwhile, the ICG photothermal treatment is accompanied. The medicine solves the problem of dark toxicity of the HP, and also realizes the combination of the controlled photodynamic treatment and the photothermal therapy through the switch effect of the ICG.

Description

Technical field [0001] The present invention is in the biomedical field, it relates to a novel, controlled photodynamic therapy of PAMAM / hematoporphyrin / indocyanine green (CPHI) Preparation and application. Background technique [0002] Photodynamic therapy (PDT) as a minimally invasive treatment, have been used in the treatment of various cancers. The process is, at a specific wavelength of the laser, photosensitizer molecule (PSs in) in the cancer cells are excited, transfer energy to ambient oxygen, resulting in highly toxic reactive oxygen species (of ROS), thereby killing the tumor cells. And conventional chemotherapy, surgery and radiotherapy compared to invasive characteristics PDT is selective for tumor cells, resulting in tumor cell necrosis or apoptosis, no damage (Kessel D surrounding normal cells and tissues, Luo Y. Mitochondrial photodamage and PDT -induced apoptosis [J] .Journal of Photochemistry & Photobiology B Biology, 1998, 42 (2): 89-95).. Hematoporphyrin (...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/69A61K47/61A61P35/00
CPCA61K41/0052A61K41/0071A61K2300/00
Inventor 高瑜李子颖吕婷婷王秀英阙榕槟马士杰陈海军
Owner FUZHOU UNIV