Application of a kind of rape stalk biochar in mediated anaerobic dechlorination of polychlorinated biphenyls

A technology of polychlorinated biphenyls and rape stalks, applied in the field of biodegradation of organic matter, can solve problems such as time-consuming, limited, and cumbersome extraction methods, and achieve an environmentally friendly effect

Active Publication Date: 2020-06-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the extraction method of humin is very cumbersome and time-consuming, and each extraction and purification takes one to two months
Also, the electronic mediation effects of humin are often limited by soil or sediment sources

Method used

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  • Application of a kind of rape stalk biochar in mediated anaerobic dechlorination of polychlorinated biphenyls
  • Application of a kind of rape stalk biochar in mediated anaerobic dechlorination of polychlorinated biphenyls
  • Application of a kind of rape stalk biochar in mediated anaerobic dechlorination of polychlorinated biphenyls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: A biochar degradation system for rape stalks, made by mixing the following components: 1 L of inorganic salt medium, 1 mg of anaerobic microorganisms, 1.5 ml of sodium lactate, 1 mL of vitamin solution, and 4 g of rape stalk biochar.

[0033] In terms of mass percentage, the anaerobic microorganisms are composed of the following bacterial species: Desulfuromonas sp. 43.5%, Desulfitobacterium sp. 23.6% Exiguobacterium sp. 3.8%, Citrobacter sp. ) 6.2%, Alkaliphilus sp. 5.1%, Pseudomonas sp. 1.8%, Dehalobacter sp. 6.5%, Bellilineasp. 3.6%, Dehalogenimonassp. 5.9%.

[0034] The formula of the inorganic salt medium is: NH 4 Cl 1.0g / L, CaCl 2 2H 2 O 0.05g / L, MgCl 2 6H2O0.1g / L, K 2 HPO 4 0.4g / L, Se / W solution 1mL / L, SL-10 trace element solution 1mL / L, pH7.2 MOPS buffer 10mmol / L, vitamin solution 0.02ml / L, water balance.

[0035] The composition of each liter of Se / W solution is: NaOH 0.5g, NaSeO 3 ·5H 2 O 3 mg, Na 2 WO 4 2H 2 O 4mg, the balance in water....

Embodiment 2

[0038] Example 2: A biochar degradation system for rape stalks, made by mixing the following components: 1 L of inorganic salt medium, 2 mg of anaerobic microorganisms, 2.5 ml of sodium lactate, 2 mL of vitamin solution, and 6 g of rape stalk biochar.

[0039] In terms of mass percentage, the anaerobic microorganisms are composed of the following bacterial species: Desulfuromonas sp. 45.2%, Desulfitobacterium sp. 25.8% Exiguobacterium sp. 5.3%, Citrobacter .) 6.5%, Alkaliphilus sp. 4.9%, Pseudomonas sp. 2.3%, Dehalobacter sp. 3.8%, Bellilinea sp. 3.4%, Dehalogenimonas sp. 2.8%.

[0040] Others are with embodiment 1.

Embodiment 3

[0041] Example 3: A biochar degradation system for rape stalks, made by mixing the following components: 1 L of inorganic salt medium, 1.5 mg of anaerobic microorganisms, 2 ml of sodium lactate, 1.5 mL of vitamin solution, and 5 g of rape stalk biochar.

[0042] In terms of mass percentage, the anaerobic microorganisms are composed of the following bacterial species: Desulfuromonas sp. 45.5%, Desulfitobacterium sp. 21.6% Exiguobacterium sp. 5.2%, Citrobacter sp. ) 8.1%, Alkaliphilus sp. 4.7%, Pseudomonas sp. 1.8%, Dehalobacter sp. 3.5%, Bellilinea sp. 4.1%, Dehalogenimonas sp. 5.5%.

[0043] Others are with embodiment 1.

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Abstract

The invention discloses application of rape stalk biochar in anaerobic reductive dechlorination of mediated polychlorinated biphenyls. The application specifically comprises the following steps that the 25 mg / L of the polychlorinated biphenyls is placed in a rape stalk biological carbon degradation system, and is cultured for more than 30 days under a dark environment at the temperature of 30 + / -1 DEG C, wherein the rape stalk biological carbon degradation system comprises the following components of, 1 L of an inorganic salt culture medium, 1.5-2.5 mL of sodium lactate, 1-2 mL of a vitamin solution, 1-2 mg of an anaerobic microorganism, and 4-6 g of rape stem biochar. According to the application, the biological anaerobic reductive dechlorination of the polychlorinated biphenyls is promoted by using the rape stalk biochar as a solid electron mediator, the rape stalk biochar is made of natural materials and is environment-friendly, and raw materials are easy to obtain, and the application provides a new way for degrading of the polychlorinated biphenyls.

Description

technical field [0001] The invention specifically relates to the application of rape stalk biochar in mediating anaerobic reduction dechlorination of polychlorinated biphenyls, and relates to the field of organic matter biodegradation. Background technique [0002] Polychlorinated biphenyls (PCBs) is a general term for a class of chlorinated aromatic compounds that are replaced by chlorine atoms to varying degrees of hydrogen on biphenyls, including 209 homologues. Due to its good chemical stability, flame retardancy and insulation, it has been widely used in electrical equipment (such as transformers, varactors, etc.) as insulating media, carbon paper, ink, paint, lubricants, plasticizers, etc. Toxicity, environmental persistence, bioaccumulation, and long-distance migration. Because the structure of PCBs is very stable, it is difficult to be degraded in the environment. At the same time, it has low water solubility and high fat solubility, and can be enriched from the surr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02C01B32/05A62D101/22
CPCA62D3/02A62D2101/22
Inventor 章春芳张宁张冬冬杨生茂周航海张志超余新威
Owner ZHEJIANG UNIV
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