CYP2D6*10 gene polymorphism detection system and kit thereof
A gene polymorphism and detection kit technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems that affect the accuracy of detection results, difficulty in large-scale promotion and application, expensive equipment, etc. problems, to achieve the effect of meeting large sample size clinical screening, low price, and reducing testing costs
Pending Publication Date: 2018-04-20
上海赛安生物医药科技股份有限公司
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Problems solved by technology
At present, technologies such as next-generation sequencing, FLP, allele-specific oligonucleotide hybridization, allele-specific PCR, and gene chips are mainly used, but the operation process is cumbersome, and reaction tubes need to be replaced, which is prone to pollution. post-processing, affecting the accuracy of the test results
Or the instruments and equipment are expensive, and it is difficult to promote and apply them on a large scale
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[0029] The present invention is specifically described below through the examples, it is necessary to point out that the following examples are only used to further illustrate the present invention, and can not be interpreted as limiting the protection scope of the present invention, those skilled in the art can according to the above-mentioned present invention Contents Some non-essential improvements and adjustments are made to the present invention. In the following examples, unless otherwise specified, all reagents used are of analytical grade, and all reagents used can be obtained from commercial sources. For the experimental methods that do not indicate the specific conditions in the text, usually follow the conditions described in the book "Molecular Cloning Experiment Guide" published by Science Press, edited by J. Sambrook et al. in 2002, or according to the manufacturer's suggestion conditions of. Unless otherwise defined, all professional and scientific terms used ...
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Abstract
The invention relates to a CYP2D6*10 gene polymorphism detection system and a kit thereof. The kit comprises a PCR detection liquid and a SYBR GreenI mixed liquid. The PCR detection liquid comprises upstream and downstream primers and peptide nucleic acid used for detecting CYP2D6*10 gene loci. The nucleotide sequence of peptide nucleic acid is totally complementary to CYP2D6*1 wild-type gene loci, The detection system and kit are high in sensitivity and specificity and less in sample amount, and allows CYP2D6*10 gene polymorphism detection to be fast accurately performed.
Description
technical field [0001] The invention relates to a method for detecting CYP2D6*10 gene polymorphism and a detection product, belonging to the field of biotechnology. Background technique [0002] Isoquine 4'-hydroxylase (CYP2D6) is an important member of the second subfamily of the cytochrome P450 superfamily (cytochrome P450s, CYP). The activity of CYP2D6 in the population presents a four-state distribution phenomenon of intensive metabolizers (EM), intermediate metabolizers (IM), poor metabolizers (PM) and ultra metabolizers (UM). At present, more than 100 mutant genes of CYP2D6 have been found, and its mutation type determines the metabolic activity of the enzyme. CYP2D6 gene polymorphism determines the difference in drug metabolism intensity, and affects the selection of clinical drug varieties and doses. In addition to participating in the metabolism of some endogenous substances and some toxic compounds in the environment, CYP2D6 is also involved in the metabolism of ...
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IPC IPC(8): C12Q1/6883C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6883C12Q2600/156C12Q2600/106C12Q2525/203C12Q2527/107C12Q2561/113C12Q2545/114C12Q2563/107
Inventor 王明赵国玲
Owner 上海赛安生物医药科技股份有限公司