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Neuroblastoma-related tumor marker and application thereof

A technology of neuroblastoma and biomarkers, which is applied in the field of tumor diagnosis and molecular targeted therapy, can solve the problems that the clinical relevance has not been studied, and achieve the effect of important promotion and application prospects and far-reaching clinical significance

Active Publication Date: 2018-04-20
徐州维康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the clinical relevance of PHF20 in neuroblastoma is still largely unstudied, and some studies have reported that it is an oncogene. Therefore, it is particularly important to explore its application in tumor markers

Method used

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  • Neuroblastoma-related tumor marker and application thereof
  • Neuroblastoma-related tumor marker and application thereof
  • Neuroblastoma-related tumor marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Comparison of the relationship between the expression of PHF20 gene and the survival rate in patients with neuroblastoma.

[0024] The Cancer Genome Atlas (TCGA) database contains multiple levels of data information for various tumor types, including: miRNA, mRNA, protein profile, mutation profile, clinical diagnosis information, etc. These data provide a rich resource for tumor data analysis. Therefore, we first analyzed the correlation between PHF20 gene and cancer with the help of TCGA database.

[0025] (1), from two databases (database one is Therapeutically Applicable Research to Generate Effective Treatments [TARGETS], the number of patients n=149, the link is http: / / cancergenome.nih.gov / ,Database 2 is the unpublished data of Texas Children’s Hospital [Texas Children’s Hospital], the number of patients (n=88) randomly selects patients with neuroblastoma, and the tumor tissue of the patients has been detected by RNA-seq to detect the expression lev...

Embodiment 2

[0028] Example 2: Using immunohistochemical staining to compare the difference in the expression of PHF20 gene between tumor tissue and normal tissue.

[0029] (1) Randomly select clinically surgically resected nerve tissues for paraffin-embedded sectioning.

[0030] (2) Patients with stage I, stage II and stage III neuroblastoma were randomly selected, tumor tissues were taken for paraffin-embedded sections, and immunohistochemical staining was performed using PHF20 antibody (purchased from Cell Signaling Technology Company, Cat. No. 3934).

[0031] (3) Results: The expression ratio of PHF20 in tumor cells of patients with neuroblastoma was significantly higher than that in normal nerve tissue, and the expression ratio of PHF20 was higher in stage III patients than in stage II patients, and the expression ratio of PHF20 in stage II patients was higher than that in stage I patients ( figure 2 ).

Embodiment 3

[0032] Embodiment 3: PHF20 detection kit detects the expression of PHF20 gene and different neuroblastoma cell lines.

[0033] Detect the expression status of PHF20 gene in 10 different cells, which are: 1 normal human peripheral blood cell PBMC, representing normal samples; 9 neuroblastoma cell lines CHLA-255, JF, LA-N-6 , NB-19, NGP, SK-N-AS, SH-EP, SH-SY5Y and IMR-32 ( image 3 ).

[0034] The detection process is as follows:

[0035] Extract the total protein samples of 10 kinds of cells, and use Western Blot to characterize the total level of PHF20 in the 10 kinds of samples. The detection results are as follows image 3 Shown in A: The results show that the total level of PHF20 in neuroblastoma cells is significantly increased compared with normal cells. Therefore, the expression level of PHF20 combined with the evaluation of clinical indicators can be used as a reference for judging whether it is neuroblastoma.

[0036] Total RNA was extracted from neuroblastoma tum...

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Abstract

The invention discloses a neuroblastoma-related tumor marker PHF20 and application of a kit for detecting the expression level of PHF20 to neuroblastoma detection and diagnosis or prognostic patient evaluation, and belongs to the field of tumor diagnosis and molecular targeted therapy. The expression level of the PHF20 is closely related to clinicopathological parameters and patient prognosis, sothat the PHF20 can be used as a specific tumor marker for early detection of neuroblastoma or a prognostic index.

Description

technical field [0001] The invention belongs to the field of tumor diagnosis and molecular targeted therapy, and relates to a neuroblastoma-related tumor marker and its application. Background technique [0002] Neuroblastoma (NB) is a neuroendocrine tumor that mainly originates from the sympathetic ganglia in the adrenal medulla, upper neck, and thorax. The clinical manifestations are characterized by high malignancy, diversity, and easy metastasis. Common childhood extracranial solid tumors. The disease mostly occurs in children under the age of 10, accounting for about 8-10% of childhood tumors, and the annual incidence rate is about 1 / 100,000, which is only lower than leukemia and central nervous system tumors. Although a few can be relieved spontaneously, most of them Most of them have a poor prognosis. Neuroblastoma has obvious heterogeneity and is prone to drug resistance to chemotherapy. At present, in the treatment of neuroblastoma, the international general tende...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K31/7105A61K48/00A61P35/00
CPCA61K31/7105C12Q1/6886C12Q2600/166C12Q2600/178G01N33/57434
Inventor 赵蔚何秋萍
Owner 徐州维康生物科技有限公司
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