Hybridization buffer solution for hybrid capture of DNA sequencing library

Abstract

The invention discloses a hybridization buffer solution for hybrid capture of a DNA sequencing library. The hybridization buffer solution is prepared from the following components in parts by volume:25 parts of 2*SSC, 1 part of 1*Denhardt's, 9 parts of SDS with mass percent concentration of 0.5%, and 14 parts of dextran solfate with mass percent concentration of 10%. The hybridization buffer solution has a simple formula, is easy to prepare, is low-cost, is stable and is easy to store; the hybridization method is simple, is easy to operate and needs no special instruments; the hybridization effect is good, the background value is low, so that the sample capture efficiency is improved; and the method is easy to implement, and uses no extra reagents and operations, so that cost loss causedby over long hybridization time is reduced, and the processing time is shortened.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a hybridization buffer for DNA sequencing library hybridization capture, which greatly improves capture efficiency and saves time. technical background [0002] The DNA template required by the existing DNA library hybridization system needs 600ng, and the hybridization time ranges from 16h to 72h, and the hybridization time is relatively long. [0003] Enrichment based on the principle of PCR, as the name implies, is to use PCR primers for the target region to amplify the target sequence, and then to sequence the specific PCR products. This method usually requires many cycles of denaturation, annealing and extension, often resulting in non-specific products and biased amplification. Additionally, for running hundreds or thousands of singleplex PCR reactions, dedicated automation or compartmentalization is required. Another option is to perform limited multiplex PCR...

AI Technical Summary

Problems solved by technology

[0007] Although the solid-phase chip hybridization method has many advantages over PCR amplification and enrichment, the cost of the chip is relatively high, and the test process requires expensive hardware equipment, such as a chip incubator

In addition, since the chips that start hybridization at the same time must elute the enriched DNA library at the same time, the number of chips that can be processed at the same time is also very limited.

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