Relay type fluorescence detection and use method of imidazole derivative mercury ions and sulfur ions

A technology of imidazole derivatives and fluorescence detection, applied in the field of relay fluorescence detection of mercury ions and sulfide ions, can solve the problem of fewer fluorescent probes, achieve strong anti-interference ability, stable fluorescence performance, simple and fast detection operation Effect

Active Publication Date: 2018-05-04
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is to solve the existing relay fluorescence detection of Hg in aqueous solution 2+ and S 2– There are very few fluorescent probes for Hg 2 + Recognized by Au 3+ , Fe 2+ and Fe 3+ affect the problem, thus providing an imidazole derivative class Hg 2+ and S 2– Relay fluorescence detection and usage method

Method used

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  • Relay type fluorescence detection and use method of imidazole derivative mercury ions and sulfur ions
  • Relay type fluorescence detection and use method of imidazole derivative mercury ions and sulfur ions
  • Relay type fluorescence detection and use method of imidazole derivative mercury ions and sulfur ions

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Embodiment 1

[0016] Use HEPES buffer solution with pH=7.4 as solvent, and the measured concentration is 1.0×10 –5 Fluorescence emission spectra of mol / L L solution for 16 common cations. Add 6.0 equiv. of 16 cations (Al 3+ , Zn 2+ , Ag + , Ca 2 + , Mg 2+ , Fe 3+ , Hg 2+ , Pb 2+ , Na + , Ba 2+ , Ni 2+ , K + , Cu 2+ , Cr 3+ , Cd 2+ , Co 2+ ) solution, only 6.0equiv. Hg 2+ It can significantly quench L fluorescence, and the quenching range is 83%. In contrast, other metal ions caused only small changes. It shows that the fluorescent probe L of the present invention has an effect on Hg 2+ Features single-selection fluorescence detection. Where: the ordinate represents the fluorescence intensity, and the abscissa represents the fluorescence wavelength.

Embodiment 2

[0018] HEPES buffer solution with pH=7.4 was used as solvent, and different concentrations (0~11.0 equiv.) of Hg 2+ Join to 1.0 x 10 –5 mol / L L solution, observe the change results of its fluorescence emission spectrum. With Hg 2+ As the concentration increases, the fluorescence intensity of L at 370 nm decreases gradually. The above results show that the effect of L on Hg 2+ Has a high sensitivity. Where: the ordinate represents the fluorescence intensity, and the abscissa represents the fluorescence wavelength.

Embodiment 3

[0020] At a concentration of 1.0 x 10 –5 Add 12.0 equiv. of 15 kinds of cations (Al 3+ , Zn 2+ , Ag + , Ca 2+ , Mg 2+ , Fe 3+ , Pb 2+ , Na + , Ba 2+ , Ni 2+ , K + , Cu 2+ , Cr 3+ , Cd 2+ , Co 2+ ) solution, shake well and let stand for 5min, then add 6.0 equiv. Hg 2+ solution, shake well. Add 6.0 equiv. to L. The fluorescence intensity of the above 15 cations is close to that of L. When the above 15 cations and Hg 2+ coexist, and the cation concentration is Hg 2+ When the concentration is 2 times, it does not interfere with the effect of L on Hg 2+ detection, the system still has an obvious fluorescence quenching response, indicating that L has an effect on Hg 2+ The detection has excellent anti-interference performance and can be used as a detection method for Hg 2+ fluorescent quenching probes. Among them: the ordinate indicates the fluorescence intensity, the abscissa indicates the metal ion species, (0) blank, (1) Al 3+ , (2) Zn 2+ , (3) Ag + , (4)...

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Abstract

The invention relates to establishment of a relay type fluorescence detection of Hg<2+> and S<2-> and aims to solve the problems that very few fluorescence probes are used for relay type fluorescencedetection of Hg<2+> and S<2-> in an aqueous solution in the prior art and recognition of Hg<2+> is easily affected by Au<3+>, Fe<2+> and Fe<3+>. The probe 1,4-o-phenylenediamine-3-(1-amyl benzimidazole)acetanilide chloride [L] can form a stable complex with Hg<2+> in an HEPES aqueous solution, so that fluorescence quenching of L is realized, and the pH value is in the range of 2.0-11.3. The probecan be used for detecting Hg<2+> and has stable fluorescence property; the detection limit of Hg<2+> can reach 4.0*10<-7> mol/L. the compound [L-Hg<2+>] can realize fluorescence detection of S<2->. Therefore, the probe can realize relay type fluorescence detection of Hg<2+> and S<2-> in the aqueous solution and has huge application prospects in the fields of environmental science, analytical chemistry, bioscience and the like.

Description

technical field [0001] The invention relates to a relay fluorescence detection mercury ion (Hg 2+ ) and sulfide ions (S 2– ) method establishment. Background technique [0002] Over the past few decades, ion recognition has attracted enormous attention due to its pivotal roles in biology, environment, and chemistry. Although many selective fluorescent probes have been reported before, there are relatively few multifunctional fluorescent probes that can achieve relay recognition. Therefore, it is of great significance to develop recyclable, low-cost and high-performance fluorescent probes for relay recognition. [0003] The development of novel probes with selective recognition of toxic heavy metal ions and anions has attracted increasing attention. In 2016, the article "Afluorescent carboxamide ligand, having combined ionophore / fluorophoremoieties, exhibiting “On-Off” switching toward Zn published on Sensors and Actuators B by Mehdi Amirnasr et al. 2+ ion” involves the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 王丽艳田莹何显优马文辉赵冰董若瑶杨佳
Owner QIQIHAR UNIVERSITY
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