Fermented milk rich in bifidobacterium breve and preparation method thereof
A technology of Bifidobacterium breve and fermented milk, which is applied in the field of fermented milk rich in Bifidobacterium breve and its preparation, can solve the problems such as difficulty in the growth of Bifidobacterium breve, achieve large market application potential, economic value, and good taste , The preparation method is simple and easy to implement
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[0023] A method for preparing fermented milk rich in Bifidobacterium breve, the preparation method comprising the following steps:
[0024] (1) Inoculate the raw milk with Bifidobacterium breve and Leuconostoc mesenteroides BD01710 respectively, stir and mix evenly, and ferment at a temperature of 30-37°C until the titrated acidity is 70- 95°T, get fermented milk;
[0025] (2) Cool the fermented milk prepared in step (1) to 16-22° C. by stirring in an ice bath or by turning over plates to obtain cooled fermented milk;
[0026] (3) Filling the cooled fermented milk into a packaging container, then transferring it to an environment with a temperature of 2-10° C. for refrigeration and then ripening for 12-24 hours to obtain fermented milk rich in Bifidobacterium breve.
[0027] The Leuconostoc mesenteroides described in step (1) is Leuconostoc mesenteroides BD01710 (Leuconostoc mesenteroides) BD01710 (Leuconostoc mesenteroides) BD01710 of the preservation number CGMCC NO. Can r...
Embodiment 1
[0036] Raw material preparation: raw milk, consisting of 10% whole milk powder or skim milk powder, 5% sucrose and water supplemented to 100%, fully dissolved, mixed evenly, homogeneous (60°C, 22MPa), sterilized at 95°C 5min, cooled to 40°C for use.
[0037] (1) Inoculate the raw milk with Bifidobacterium breve (10 7 CFU / mL) and Leuconostoc enterica BD01710 (3×10 6 CFU / mL), stirred and mixed evenly, and left to ferment at a temperature of 30°C until the titrated acidity was 70°T to obtain fermented milk;
[0038] (2) Cool the fermented milk prepared in step (1) to 16° C. by stirring in an ice bath or by turning over plates to obtain cooled fermented milk;
[0039] (3) Fill the cooled fermented milk into a packaging container, then transfer it to an environment with a temperature of 2° C. for refrigerating and then ripening for 12 hours to obtain fermented milk rich in Bifidobacterium breve.
[0040] The above-mentioned fermented milk samples were sampled and counted by plat...
Embodiment 2
[0044] Raw material preparation: Raw milk consists of 10% whole milk powder or skim milk powder, 5% sucrose and water supplemented to 100%, fully dissolved, mixed evenly, homogeneous (70°C, 22MPa), sterilized at 95°C 10min, cooled to 45°C for use.
[0045] (1) Inoculate the raw milk with Bifidobacterium breve (10 6 CFU / mL) and Leuconostoc enteritidis BD01710 (10 7 CFU / mL), stirred and mixed evenly, and left to ferment at a temperature of 37°C until the titrated acidity was 95°T to obtain fermented milk;
[0046] (2) cooling the fermented milk prepared in step (1) to 22° C. by stirring in an ice bath or by turning over plates to obtain cooled fermented milk;
[0047] (3) Filling the cooled fermented milk into a packaging container, and then transferring it to an environment with a temperature of 10° C. for refrigerating and then ripening for 24 hours to obtain fermented milk rich in Bifidobacterium breve.
[0048] The above-mentioned fermented milk samples were sampled and c...
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