Fixed bed type cell bioreactor

Abstract

The invention discloses a fixed bed type cell bioreactor which comprises a stirring device, a ventilation device, an exhaust device, a supplementing device, a fixed bed device, a water jacket interlayer and a controller. The fixed bed device is composed of an outer ring cylindrical shell, a middle ring cylindrical shell, an inner ring cylindrical shell, a hollow cylindrical flow guiding tube, an upper cover plate, a lower cover plate and a hollow flow guiding tubule; the defect that an existing fixed bed type cell bioreactor is not sufficient in cell density and mass transfer efficiency is overcome, the bioreactor is applicable to the adherent cell cultivation mode, the nutrient solution and oxygen mass transfer efficiency can be better improved, and the higher cell density and product concentration are achieved.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a fixed-bed cell bioreactor. Background technique [0002] In recent decades, due to the sharp increase in human demand for biological products such as growth hormone, interferon, monoclonal antibodies, vaccines and interleukins, cell culture through large-scale in vitro culture technology is an effective method for the production of biological products. The so-called large-scale cell culture technology refers to the technology of cultivating animal cells at high density and in large quantities in bioreactors under artificial conditions (setting pH, temperature, dissolved oxygen, etc.) for the production of biological products. At present, animal cells that can be cultured on a large scale include primary cells such as chicken embryo, pig kidney, monkey kidney, hamster kidney, human diploid cells, Vero cells, etc., and have successfully produced vaccines including rabies vaccine...

AI Technical Summary

Problems solved by technology

However, because only the upper and lower cover plates are used to press the carrier tightly, it is difficult to discharge the metabolites and dead cells of the cells, and because the carriers are piled up together, the culture medium flows unevenly, resulting in uneven nutrient distribution and inconsistent cell growth rates.

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