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Abelmoschus manihot flower polysaccharide extracting method

A technology for extracting golden sunflower and polysaccharide, which is applied in the field of polysaccharide extraction, can solve problems such as insufficient thoroughness and complex structure, and achieves the effects of reducing loss, simple operation and convenient extraction.

Pending Publication Date: 2018-05-11
HANDAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many kinds of polysaccharides and complex structures. At present, the research on polysaccharides of goldenflower sunflower is not thorough enough, and further research is still needed by scientific researchers.

Method used

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  • Abelmoschus manihot flower polysaccharide extracting method
  • Abelmoschus manihot flower polysaccharide extracting method
  • Abelmoschus manihot flower polysaccharide extracting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] In a kind of golden flower sunflower polysaccharide extraction method, mainly comprises the following steps:

[0101] Step 1. After the sunflowers are washed, they are put into the reaction chamber of the extractor and dried to a constant weight at a temperature of 60° C. to obtain dried sunflowers, and then crushed to 60 mesh to obtain sunflower powder;

[0102] Step 2, the golden flower sunflower powder obtained is weighed quantitative distilled water according to the solid-liquid ratio 1:58.28g / mL, and the cellulase that accounts for 0.95% of the total mass of the golden flower sunflower powder, and the distilled water and cellulose Enzyme mixing and heating to a temperature of 50°C, adding to the goldenflower sunflower powder and stirring evenly, adjusting the pH value to 7, and continuously stirring for 0.81h to obtain an enzymatic hydrolysis mixture;

[0103] Step 3. After inactivating the enzymolysis mixture at a temperature of 92°C for 10 minutes, centrifuge at ...

Embodiment 2

[0108] In a kind of golden flower sunflower polysaccharide extraction method, mainly comprises the following steps:

[0109] Step 1. Wash the sunflower and store it at 6°C for 4 hours, put it into the reaction chamber of the extractor and dry it for 1.5 hours at a temperature of 60°C, then reduce the temperature of the reaction chamber to 50°C and continue drying for 3 hours. Finally, reduce the temperature of the reaction chamber to 35° C. and dry at a constant temperature to a constant weight to obtain dried sunflower sunflowers, which are crushed to 60 mesh to obtain sunflower sunflower powder;

[0110] Step 2, the golden flower sunflower powder obtained is weighed quantitative distilled water according to the solid-liquid ratio of 1:58.28g / mL, and the cellulase that accounts for 0.95% of the total mass of the golden flower sunflower powder, and the distilled water and cellulose Divide the enzyme into 3 parts equally, and heat 3 parts of distilled water to 60°C, 50°C and 65...

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Abstract

The invention discloses an abelmoschus manihot flower polysaccharide extracting method. The method comprises the following steps: gradiently drying abelmoschus manihot flowers to be constant-weight, to obtain dried abelmoschus manihot flowers, and grinding to obtain abelmoschus manihot flower powder; adding quantitative and constant-temperature enzymatic hydrolysate into the obtained abelmoschus manihot flower powder by sections and evenly stirring, adjusting pH value to 6.5-7, then continuously stirring and extracting to obtain mixed enzymolysis liquor; inactivating mixed enzymolysis liquor and centrifuging, filtering the filtrate and placing into an extracting cavity; adding ethanol into the filtrate, sealing and standing to obtain alcohol precipitated mixed liquor; centrifuging alcoholprecipitated mixed liquor, filtering to leave precipitate; sequentially washing the precipitate sequentially in 95% ethanol, absolute ethyl alcohol and acetone, drying to be constant weight at constant temperature, to obtain abelmoschus manihot flower polysaccharide. The extracting technology is optimized by combining a response surface method, the rapid extraction of abelmoschus manihot flower polysaccharide can be realized, the extraction rate is high and the extraction cost is low.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a method for extracting polysaccharides from goldenflower sunflowers. Background technique [0002] In recent years, all walks of life have paid great attention to the research on plant extraction, and many plant components have been extracted and utilized in large quantities, resulting in the emergence of many industries and the development of many industrial chains. With the improvement of people's living standards, the extraction of plant polysaccharides has become a research hotspot due to the high utilization value of polysaccharides. The herbaceous plant Hibiseumanihot L. belongs to the genus Okra of Malvaceae, and its life cycle is completed within one year. According to its growth habit and morphological characteristics, people also call it cabbage hibiscus, sticky stem, mountain elm bark and wild hibiscus, etc. Golden flower sunflower is rich in variou...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 付伟韩佳佳付静
Owner HANDAN COLLEGE
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