A kind of extraction method of non-denatured protein and its special extracting solution

An extraction method and a non-denaturing technology, which are applied in the field of non-denaturing plant protein extraction and its special extraction solution, can solve the problems of inability to meet the non-denatured protein extraction requirements of Populus euphratica leaves, increasing the risk of dissolving pigment molecules, and interfering with electrophoresis patterns. , to achieve the effect of great application value, mild reagents, and efficient extraction

Active Publication Date: 2020-08-11
MINZU UNIVERSITY OF CHINA
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the existing traditional protein extraction methods cannot meet the requirements for the extraction of non-denatured proteins from Populus euphratica leaves, especially the research on membrane proteins that require protein solubilization is even less applicable, and the introduction of non-ionic detergents will increase the pigment molecules at the same time. Risk of dissolution, severely interferes with electropherograms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of extraction method of non-denatured protein and its special extracting solution
  • A kind of extraction method of non-denatured protein and its special extracting solution
  • A kind of extraction method of non-denatured protein and its special extracting solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0049] Example, comparison of 5 non-denatured protein extraction methods

[0050] The inventor of the present invention collected whole and insect-free fresh Populus euphratica leaves from the natural Populus euphratica forest (101°16'54.60"E, 42°2'35.67"N) from Badaoqiao, Ejina Banner, Alxa League, Inner Mongolia, and then quickly frozen in liquid nitrogen. A sample of Populus euphratica leaves was obtained.

[0051] 1. Use 5 methods to extract the non-denatured protein of Populus euphratica leaf samples

[0052] A, imidazole method

[0053] 1. Place 1g of Populus euphratica leaf sample in a mortar, add 100mg PVPP and a small amount of quartz sand, and grind thoroughly with liquid nitrogen to obtain powder.

[0054] 2. After completing step 1, place the powder in another new mortar and add 3 mL of extract to homogenize thoroughly to obtain a homogenized solution. The extract is a 0.001 mol / L EDTA buffer containing 0.05 mol / L imidazole, 0.002 mol / L 6-aminoacetic acid and 3% (3 mg / 100 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an extraction method of non-denatured protein and extraction liquid specially used by the method. The extraction method comprises a step a2): extracting the non-denatured protein in a sample by adopting the extraction liquid, wherein the extraction liquid contains sucrose, NaCl, L-sodium ascorbate, digitonin, dithiothreitol, phenylmethylsulfonyl fluoride and a protease inhibitor. The extraction method provided by the invention is easy and convenient to operate, a reagent is mild and economic, high-quality non-denatured protein can be efficiently extracted, the method lays a foundation and provides technical support for subsequently performed adverse plant proteomics research, and the method has a certain guiding significance for extracting recalcitrant tissue protein of plants living in an extreme environment. The method has a significant application value.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for extracting non-denatured protein and a special extract solution thereof, in particular to a method for extracting non-denatured plant protein and a special extract solution thereof. Background technique [0002] As the main bearer of life activities, proteins mostly exist in the form of protein complexes and interacting proteins in cells, and their structures and functions are very complex. Traditional two-dimensional electrophoresis can only distinguish the denatured proteins extracted from plants, not their state when they assume functions in cells, and is not conducive to the study of protein-protein interactions and protein complexes. Protein separation is the core of electrophoresis technology, and a good protein extraction method is the prerequisite and guarantee for obtaining intuitive protein visualization information. Up to now, the traditional methods fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 王晓东冯金朝石莎韩航
Owner MINZU UNIVERSITY OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap