Anti-oxidization peptide and preparation method thereof as well as cosmetic containing anti-oxidization peptide
A technology of anti-oxidative peptides and peptide segments, which is applied in the preparation method of peptides, cosmetics, cosmetic preparations, etc., can solve the problems of toxic and side effects, and achieve the effects of no toxic and side effects, mild reaction conditions, and high antioxidant activity
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[0030] The preparation method of the antioxidant peptide of an embodiment of the present invention comprises the following steps:
[0031] 1) Select the cuttlefish, remove the viscera, wash them with water, and homogenize them into a puree with a homogenizer.
[0032] In a preferred embodiment, the cuttlefish is selected from Mann-style squid, which has dense flesh and high protein content, and the polypeptide obtained after enzymatic hydrolysis has high antioxidant activity.
[0033] In a preferred embodiment, the homogenization speed of the homogenizer is 3000 rpm, and the homogeneity of the minced meat is good, which is favorable for enzymatic hydrolysis and has a high degree of hydrolysis.
[0034] 2) Add water according to the ratio of material to liquid of 1:3-1:5 (mass-volume ratio is m / v) to obtain a mixed solution, adjust the pH value of the mixed solution to 7.0-8.0, add protease for enzymatic hydrolysis, and the amount of protease added is per gram Add 500U-1500U t...
Embodiment 1
[0068] 1) Select the cuttlefish, remove the viscera, wash them with water, and homogenize them into a puree with a homogenizer.
[0069] 2) Add water according to the ratio of material to liquid of 1:3 (m / v) to obtain a mixed solution, adjust the pH value of the mixed solution to 7.0, add protease for enzymatic hydrolysis, the amount of protease added is 1000U per gram of meat puree, among which, trypsin The mass ratio of papain to the papain is 1:3, enzymolysis treatment is carried out at 40°C for 2 hours, the enzyme is inactivated, centrifuged, and the supernatant is taken.
[0070] 3) The supernatant was separated with a 20,000 Da ultrafiltration membrane, and the ultrafiltrate with a molecular weight below 20,000 Da was taken and freeze-dried to obtain peptide powder A1.
[0071] 4) Peptide powder A1 was dissolved in distilled water at 20 mg / mL, separated and purified by Sephadex G-25 chromatography column, the purified solution with a molecular weight between 400-1600Da w...
Embodiment 2
[0073] 1) Select the cuttlefish, remove the viscera, wash them with water, and homogenize them into a puree with a homogenizer.
[0074] 2) Add water according to the ratio of material to liquid of 1:4 (m / v) to obtain the mixed solution, adjust the pH value of the mixed solution to 7.5, add protease for enzymatic hydrolysis, the amount of protease added is 800U per gram of meat puree, among which, trypsin The mass ratio of papain to the papain is 1:4, enzymolysis treatment is carried out at 50°C for 2.5 hours, the enzyme is inactivated, centrifuged, and the supernatant is taken.
[0075] 3) The supernatant was separated with a 20,000 Da ultrafiltration membrane, and the ultrafiltrate with a molecular weight below 20,000 Da was taken and freeze-dried to obtain peptide powder A2.
[0076] 4) Peptide powder A2 was dissolved in distilled water at 20 mg / mL, separated and purified by Sephadex G-25 chromatography column, the purified solution with a molecular weight between 400-1600D...
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