Isothermal thermal power measuring device with fluorescence spectrum measuring function

Abstract

The invention relates to an isothermal thermal power measuring device with a fluorescence spectrum measuring function. The isothermal thermal power measuring device with the fluorescence spectrum measuring function is characterized by comprising a thermal measuring system, a fluorescence spectrum measuring system and a data collecting-controlling system; the thermal measuring system is used for measuring titration heat released during titration, the fluorescence spectrum measuring system comprises a fluorescence light source, two signal incident end fiber coupling lenses and corresponding electric translation stages, two signal receiving end fiber coupling lenses and corresponding electric translation stages, gratings, and a spectrometer, wherein the data collecting-controlling system adjusts the widths of entrance and exit slits of a monochromator to select wavelengths so as to adjust the fluorescence excitation monochromatic light intensity and bandwidth. According to the invention,a light path is placed outside a reaction tank, so that the isothermal thermal power measuring device is simpler, more convenient and faster to operate; a sample pool and a reference pool are simultaneously used for performing the double sub-pool heat measurement and dual-path spectrum measurement, so as to eliminate the influence of heat signals generated by light absorption in the spectrum measurement on the thermal measurement, and effectively improve the light measurement efficiency.

Description

technical field [0001] The present invention relates to a thermal power measurement device, in particular to an isothermal thermal power measurement device with fluorescence spectrum measurement function, and relates to the research and development of weak interactions between molecules such as biological macromolecules and molecular self-assembly and other solution reaction systems. Field of application technology. Background technique [0002] Thermal power measuring devices (ie, calorimeters) are widely used in scientific fields such as physics, chemistry, and biology, as well as technical fields such as oil exploration, petrochemicals, and secondary batteries. Among them, the isothermal titration calorimeter is especially suitable for the study of biological systems and weak interactions between molecules. Due to the complexity of this type of system, it is generally supplemented by fluorescence spectrum measurement to obtain material structure information. Isothermal ...

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Problems solved by technology

This makes it often difficult to obtain a strict correspondence between the interaction and the material structure by performing titration calorimetry and fluorescence spectrometry respectively.

So far, there is no instrument at home and abroad that can realize the joint measurement of thermal signal measurement and fluorescence spectrum

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