Method for determining content of three flavone components in kidney-tonifying and toxin-drawing granule
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A method for determination and granule technology, which is applied in the content determination of puerarin, hyperoside and icariin, and the content determination of three flavonoid components in Bushen Tuodu Granules, which can solve the problem of simultaneous determination of puerarin and other issues, to achieve the effect of easy popularization and implementation, and simple methods
Active Publication Date: 2018-05-22
石家庄平安医院有限公司
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However, there is no report on the simultaneous determination of puerarin, hyperin and icariin in traditional Chinese medicine preparations
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Embodiment 1
[0042] (1) Chromatographic conditions and system suitability test Acetonitrile is phase A, methanol-0.1% phosphoric acid (1:30) is phase B, and gradient elution is used, that is, 0-20min, 8-25%A; 20-35min, 25~42%A; 35~35.1min, 42~8%A; 35.1~40min, 8%A; flow rate 1.0mL / min; column temperature 40℃; detection wavelength: 263nm; Not less than 3000;
[0043] (2) Preparation of reference substance solution Precisely weigh the appropriate amount of puerarin, hyperin and icariin reference substances, put them in a brown measuring bottle, add methanol to make each 1ml contain 0.09mg of puerarin and hyperin respectively A mixed reference solution of 0.009mg and icariin 0.09mg;
[0044] (3) Preparation of the test solution Take this product, grind it finely, take 0.4g, accurately weigh it, put it in a conical flask, accurately add 25ml of 60% methanol, weigh it, and process it with a power of 250W and a frequency of 33kHz for 10 Minutes; let cool, weigh again, make up the lost weight wi...
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Abstract
The invention relates to a method for determining the content of three flavone components in a kidney-tonifying and toxin-drawing granule, that is a method for determining the content of puerarin, hyperoside and icariin. The method is characterized in that four ratio change gradient elution is carried out with acetonitrile as a phase A and methanol-0.1% phosphoric acid (1:30) as a phase B, and theflow velocity is 1.0 mL/min; the column temperature is 40 DEG C; and the detection wavelength is 263 nm. The puerarin content, the hyperoside and the icariin content of the kidney-tonifying and toxin-drawing granule are simultaneously determined. The wave peak retention time of the puerarin is 9.5 min, the wave peak retention time of the hyperoside is 15.5 min, the wave peak retention time of theicariin is 28.5 min, the peak appearance of the three components is finished within 30 min, and all wave peaks are well separated. The method has the advantages of simplicity, fastness, high efficiency, low cost, and easiness in promotion and knowing.
Description
technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicines, and relates to a content determination method of three flavonoid components in Bushen Tuodu Granules, that is, a content determination method of puerarin, hyperin and icariin. Background technique [0002] Bushen Tuodu Granules are composed of epimedium, psoralen, dodder, medlar, rehmannia glutinosa, dandelion, knotweed, astragalus, kudzu root, ginseng and notoginseng, and eleven herbs. It has the effects of nourishing kidney and essence, nourishing qi and blood It is used for dizziness, palpitation, shortness of breath, pale complexion, bleeding of skin and mucous membranes, heavy menstruation, weak waist and knees caused by deficiency of spleen and kidney, deficiency of qi and blood, etc.; aplastic anemia, iron deficiency Patients with chronic anemia, giant cell anemia, hemolytic anemia, leukemia, myelodysplastic syndrome, thrombocytopenic purpura, multiple myelom...
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