A stool lactose detection kit and its application and detection method

A technology for detection kits and kits, which is applied in biochemical equipment and methods, measuring devices, microbe determination/inspection, etc., can solve problems such as poor specificity, difficulty in automation, and complicated clinical operations, and achieve simple, fast, No effects of radioactivity or lead contamination

Active Publication Date: 2021-02-23
CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pH-lead acetate method and the Benedictine reagent method are currently the two most widely used methods for this project in China, which can be used for semi-quantitative detection of disaccharide products in feces, but both methods require sample pretreatment and high-temperature water bath Heating is more complicated in clinical operations, has poor specificity, and is difficult to automate, and the pH-lead acetate method also has problems such as lead pollution. Although it is widely used, its defects are also obvious.

Method used

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  • A stool lactose detection kit and its application and detection method
  • A stool lactose detection kit and its application and detection method
  • A stool lactose detection kit and its application and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation of kit of the present invention

[0031] 1. Preparation of test strips

[0032] 1.69% w / w glucose oxidase, 0.2% w / w horseradish peroxidase, 0.7% w / w lactase, 0.1% w / w chromogenic chromogen, 71.5% w / w buffer and 25.81% After the non-reactive substances are fully dissolved, the soaking stock solution is prepared.

[0033] Soak 300*270*0.2mm filter paper in the original solution, take out the filter paper after fully absorbed, scrape off the excess liquid on the surface, put it on the drying net and put it in an oven at 70℃ for about 1.5h.

[0034] Paste the dried filter paper with double-sided tape, cut it into strips with a width of 3 mm with a cutter, stick it on the polyester film, and cut it into test strips with a width of 5 mm. After the cutting is completed, pack it in a black tube, put in a desiccant, seal it with an aluminum foil machine, and store it away from light.

[0035] 2. Preparation of standard color chart

[0036] Prepa...

Embodiment 2

[0037] Embodiment 2: the detection process of kit of the present invention

[0038] Take 2g of the feces sample to be tested and place it in a pre-diluted container; add 2ml of distilled water; stir and mix the sample in distilled water; filter through a filter, take 0.5mL of the filtered liquid, apply the sample on a test strip, and develop color after 1min , compared with the standard color card or obtained with a reflectance colorimeter.

Embodiment 3

[0039] Embodiment 3: clinical detection test

[0040] The feces of a number of volunteers were randomly selected in Chongqing Children's Hospital to be tested according to the ONPG method and the detection method of the kit of the present invention, and the results are shown in Table 2.

[0041] Table 2

[0042]

[0043]

[0044] It can be seen from Table 2 that the detection according to the method of the kit of the present invention can obtain the lactose concentration in the sample more accurately, which in turn can help professionals infer the level of lactase activity in the body.

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Abstract

The invention relates to the technical field of kit detection, and discloses a stool lactose detection kit as well as application and a detecting method thereof. The kit comprises a test paper strip and a standard color card, wherein the test paper strip is prepared from glucose oxidase, horse radish peroxidase, a developing color base, a buffer solution and nonreactive substances. The invention discloses a novel stool lactose detection process of a reaction process based on enzymatic reaction and color-development principle through a dry-process chemical method, and the whole process is simple, convenient and quick, does not need to perform special treatment on faeces sample, is free of radioactive or lead pollution, can realize semi-quantitative determination on concentration of lactose,so that enzyme activity of the lactase is detected.

Description

technical field [0001] The invention relates to the technical field of kit detection, in particular to a fecal lactose detection kit and its application and detection method. Background technique [0002] Lactase (Lactas), namely β-galactosidase (β-galactosidas), is a disaccharidase present on the surface of the microchorion of the small intestinal mucosa of mammals, which can hydrolyze lactose in milk into glucose and galactose; Glucose is the source of energy for human metabolism, while galactose is a component of the brain and nervous tissue glycolipids, and is an essential substance for the development of the baby's brain. When lactase activity is reduced due to congenital lactase deficiency or other reasons, lactose cannot be decomposed and absorbed, and clinical symptoms such as borborygmus, abdominal pain, rectal gas, and osmotic diarrhea occur, it is called lactose intolerance, and there are no clinical symptoms It's called lactase deficiency or lactose malabsorptio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/34G01N2333/902G01N2333/908G01N2333/924
Inventor 李小强贾彦军陈大鹏陈曦
Owner CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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