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Extraction method of natural paclitaxel

A technology of natural paclitaxel and extraction method, which is applied in the field of extraction of natural paclitaxel, and can solve the problems of resource and environmental damage, large amount of organic synthesis of paclitaxel, etc.

Pending Publication Date: 2018-06-01
SHANGHAI JINHE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, obtaining paclitaxel by this method requires a large amount of organic synthesis, which causes great damage to resources and the environment.

Method used

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  • Extraction method of natural paclitaxel
  • Extraction method of natural paclitaxel
  • Extraction method of natural paclitaxel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] figure 1 It is a process flow diagram of the present invention, such as figure 1 Shown, a kind of extraction method of natural paclitaxel comprises the following steps:

[0040] 10kg of crushed Taxus yunnanensis branches and leaves are evenly and continuously input from the entrance of the extraction equipment for 10 minutes, and the prepared 200kg mixed aqueous solution (0.1% acetic acid, 2.0% acetone aqueous solution) extract is injected into the extraction equipment at a constant speed at the same time, and the soaking solution flows to 1.0 ton storage tank, the soaking liquid is recovered and then transferred to the next batch of extraction procedures. After the solid extraction residue is squeezed by a press to remove most of the water, it is turned back into the extraction equipment, and 60kg of methanol is injected, all according to 10 minutes Feed at a complete speed, after the extraction is completed, concentrate the extract under reduced pressure until no liq...

Embodiment 2

[0042] A method for extracting natural paclitaxel, comprising the following steps:

[0043] 100kg of crushed Taxus yunnanensis branches and leaves are evenly and continuously input from the entrance of the extraction equipment for 80 minutes, and the prepared 2000kg mixed aqueous solution (0.1% acetic acid, 2.0% acetone aqueous solution) extract is injected into the extraction equipment at a constant speed at the same time, and the soaking solution flows to 10 tons of storage tank, the soaking liquid is recovered and then transferred to the next batch of extraction procedures. After the solid extraction residue is squeezed by a press to remove most of the water, it is turned back into the extraction equipment, and 600kg of methanol is injected, all according to 80 minutes Feed at a complete speed. After the extraction is completed, the extract is concentrated under reduced pressure until no liquid drops out. After 100L of acetone is dissolved, it is prepared with AB-8 resin. Th...

Embodiment 3

[0045] A method for extracting natural paclitaxel, comprising the following steps:

[0046]1.0 tons of crushed yew branches and leaves of Yunnan yew were fed continuously and evenly for 8 hours from the entrance of the extraction equipment, and 20 tons of the prepared mixed aqueous solution (0.1% acetic acid, 2.0% acetone aqueous solution) extract was injected into the extraction equipment at a uniform speed at the same time, and the soaking solution Self-flow to 50-ton storage tank, the soaking liquid is recovered and then transferred to the next batch of extraction procedures. After the solid extraction residue is squeezed by a press to remove most of the water, it is turned back into the extraction equipment, and 6.0 tons of methanol is poured into it. Feed according to the speed of 8 hours. After the extraction is completed, the extract is concentrated under reduced pressure until no liquid drips out. After 1000L of acetone is dissolved, it is prepared with AB-8 resin, and ...

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Abstract

The invention discloses an extraction method of natural paclitaxel. The method comprises steps as follows: (1), water-soluble impurities and greasy compounds are removed from branches and leaves of Taxus chinensis by adopting a mixed solvent with water as a main component; (2), an analogue with natural paclitaxel as a main component is extracted by an alcoholic solvent from solid slag obtained inthe step (1); (3), a crude product of natural paclitaxel extract is rapidly eluted and purified by macroporous resin; (4), high-purity natural paclitaxel is obtained through crystallization and refining. The technological method utilizes few organic solvents and is environmentally friendly and simple to operate, equipment is simple, large-scale production is easy to realize, the whole production process is low in loss, comprehensive utilization of resources is facilitated, product purity reaches 98% or higher, and the maximum content of individual impurities is lower than 0.1%.

Description

technical field [0001] The invention relates to a process for extracting natural antitumor drugs, in particular to a preparation method for extracting natural paclitaxel. Background technique [0002] Paclitaxel is a secondary metabolite extracted from yew plants. Paclitaxel mainly acts on the tubulin system. It can promote the assembly of tubulin into microtubules and inhibit the depolymerization of microtubules, resulting in abnormal arrangement of vascular bundles. Asteroids are formed, the spindle loses its normal function, and cancer cells die. Clinically, it is mainly used to treat ovarian cancer, breast cancer and non-small cell lung cancer, and its anti-tumor activity is higher than that of commonly used anti-tumor drugs such as thiazofurin, doxorubicin and fluorouracil. Therefore, paclitaxel has become the most widely used anticancer drug because of its broad-spectrum and high anticancer activity. [0003] In 1963, American chemists M.C.Wani and Monre E.Wall first...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14
CPCC07D305/14
Inventor 张伟中王权勇蔡志霞谢斌
Owner SHANGHAI JINHE BIO TECH
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