3D cell culture chip based on soft lithography as well as preparation method and application of 3D cell culture chip

Abstract

The invention discloses a 3D cell culture chip based on soft lithography as well as a preparation method and an application of the 3D cell culture chip. The preparation method comprises steps as follows: a mask provided with a set pattern structure is manufactured with the soft lithography; the mask is uniformly covered with a liquid polymer compound or a polymer compound solution, and a liquid layer with a certain thickness is formed; after the liquid layer is cured, the mask is removed, and a seal with a set 3D structure arranged on the printing surface is obtained; the printing surface is contacted with a modifier, so that the modifier can be attached to the printing surface in a separable manner; the printing surface and the surface of a selected substrate are contacted, then, the sealis moved away from the selected substrate, so that at least part of the modifier is separated from the printing surface and attached to the surface of the selected substrate to form a pattern structure, wherein the pattern structure comprises a plurality of arrays formed by patterns capable of adsorbing single cells. The 3D cell culture chip is easy and simple to prepare on a large scale at a lowcost and can provide an ideal 3D cell culture environment.

Description

technical field [0001] The invention relates to a biological chip, in particular to a three-dimensional cell culture chip based on soft photolithography technology, its preparation method and application. Background technique [0002] In the current biological and medical research, the first choice is to conduct cytological tests to detect drugs, and in most cases, the traditional two-dimensional cell adherence culture method is used, which is different from the existence of real tissue cells in the body. States (three-dimensional states and with ECM environment) are essentially different. Cells in organisms grow in a three-dimensional microenvironment, while two-dimensional cell culture is not the natural state of cell growth. The culture microenvironment is too different from the in vivo microenvironment, which affects the gene expression and signal transduction of cells. As a result, the cultured cells gradually lose their biological characteristics and functions in vivo...

AI Technical Summary

Problems solved by technology

[0004] (1) It is impossible to provide an effective ECM culture environment for cells. Most of them only rely on the three-dimensional structure to provide a 3D culture space. Premature aggregation leads to cell hypoxia and impeded material metabolism, eventually leading to cell apoptosis or inhibiting proliferation and stem cell differentiation;

[0005] (2) It is impossible to precisely control the properties and number of cells of the three-dimensional cell spheroids, and it is also impossible to maintain the homogeneity of the cells. It can only achieve three-dimensional culture at random positions, and it is difficult to position observation and analysis, and the throughput is low. In drug screening The application of the aspect has great limitation;

[0006] (3) Generally, cells need to be in direct contact with organic compounds, and it is difficult to rule out damage to cell proliferation or differentiation

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